Simultaneous determination of ginsenoside Rb1, ginsenoside Rg1, paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma by liquid chromatography–tandem mass spectrometry: Application to a pharmacokinetic study of wen‐Yang‐Huo‐Xue soft capsule

Abstract

A simple and sensitive HPLC-MS/MS method was developed and fully validated for simultaneous determination of ginsenoside Rb1, ginsenoside Rg1, paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma. Plasma samples were pretreated with protein precipitation using acetonitrile. The chromatographic separation was carried out on a C column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid). All analytes and digoxin (internal stand, IS) were quantitated through electrospray ionization in negative ion multiple reaction monitoring mode. All calibration curves exhibited good linearity (r > 0.9960) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were all <12.0% and the accuracies (RE) ranging from -6.1 to 6.2%. The extraction recoveries of the five compounds ranged from 89.2 to 97.1%. The validated method was successfully applied in a comparative pharmacokinetic study of Wen-Yang-Huo-Xue soft capsule (WYHXSC) in rats. Compared with single pure component, the exposure of the investigated components, except for oxypaeoniflorin, increased after oral administration of WYHXSC in rats, which suggested a synergistic effects between the herbs in the WYHXSC preparations.

Allium macrostemon whole extract ameliorates obesity-induced inflammation and endoplasmic reticulum stress in adipose tissue of high-fat diet-fed C57BL/6N mice

“…In rat model, a Chinese herbal medicine named Wen-Yang-Huo-Xue formula containing AME at 21% of total composition was orally administrated and rat plasma was analyzed for pharmacokinetics of active compounds including ginsenoside Rb1, ginsenoside Rg1, paeoniflorin, albiflorin and oxypaeoniflorin. The last three saponins were absorbed rapidly with peak time within 1 h and were eliminated quickly ( 63 ). In the other study, seven active constituents of AME including six types of steroidal saponins such as smilagenin, gitogenin, and macrostemonoside D as well as one flavonoid, naringenin, were evaluated for the ADME properties.…”

Quantification of 10 bioactive components of Yazhangsan in rat plasma by LC–MS/MS and its application

“…In recent years, multiple components have been confirmed to be responsible for the therapeutic effects of traditional Chinese medicine (TCM) (Alolga et al, 2015; Lan, Xie, & Jia, 2013). Pharmacokinetics studies of TCM are an effective way to elucidate the mechanism of their effects, and such studies are essential in TCM drug development and provide useful data for predicting and explaining the efficacy and toxicity of TCM (Wang et al, 2013; Wu, You, Qu, Ma, & Zhang, 2017). To clarify the pharmacokinetic characteristics of multiple components of YZS, an LC–MS/MS method was developed and fully validated for the measurement of ephedrine, pseudoephedrine, methylephedrine, glycyrrhizic acid, glycyrrhetinic acid, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin and IAsp ‐N‐ Glc of YZS in rat plasma for the first time in this study.…”