Simultaneous determination of haloperidol and bromperidol and their reduced metabolites by liquid–liquid extraction and automated column-switching high-performance liquid chromatography

Bioanalytical methods for the determination of antipsychotic drugs

“…After clean-up, a six-port valve is switched to connect the clean-up column with the analytical column. Using column switching with an on-line sample clean-up may be even more advantageous, since it enables automated sample analysis for routing clinical samples (Weigmann et al, 2001;Sachse et al, 2003Sachse et al, , 2006Yasui-Furukori et al, 2004;Kollroser and Schober, 2002;Olesen and Poulsen, 1993). Weigmann et al (2001) used a cyanopropyl (CPS) coated column (10 μm; 10 × 2.0 mm i.d.)…”

“…The method was suitable for routine monitoring of OLZ and CLOZ including their demethylated metabolites. Yasui-Furukori et al (2004) describes a method for determination of HAL and RHAL using automated column-switching HPLC. The test compounds were extracted from 1 mL of plasma using chloroform-hexane (30:70, v/v), and the extract was injected into a hydrophilic methacrylate polymer column for clean-up and a C 18 analytical column for separation.…”

“…The LLOQ was 0.1 ng/mL and recovery was approximately 84.0%. Some LLE methods for OLZ, HAL, CLOZ and CPZ have been reported (Gervasini et al, 2003;Nirogi et al, 2006;Hattori et al, 1986;Yasui-Furukori et al, 2004;McKay et al, 1982;Li et al, 2006;Midha et al, 1981;Zhang et al, 2007;Garay Garcia et al, 2003;Ho et al, 2004;Chung et al, 1993). Berna et al (2002) described an LC-MS/MS method for the determination of OLZ from whole blood.…”

Determination of haloperidol drug in ampoules and in urine samples using a potentiometric PVC-membrane and graphite coated wire electrodes

“…Therefore, the determination of trace amounts of HP ion in biological fluids is necessary. Several analytical methods have been reported for the assay of haloperidol including capillary electrophoresis (17), acidimetric titration in non-aqueous medium (18), uV spectrophotometry (19)(20)(21), derivative spectrophotometry (22), colorimetry (23), H-nuclear magnetic resonance spectroscopy (24), densitometry (25), gas chromatography (gC) with electron-capture detection (26) gC with surface ionization detection (27), liquid chromatography (lC) with electrochemical detection (28), lC with uV detection (29), gas-lC with nitrogen-phosphorus detection (30,31), lC with mass spectrometric (MS) detection (32,33), high-performance liquid chromatography (HPlC) with diode array detection (34), HPlC with uV-VIS detection (35,36), micellar electrokinetic chromatography (37), voltammetry (38) and potentiometry (3). These methods are highly sensitive.…”

Simultaneous determination of five antipsychotic drugs in rat plasma by high performance liquid chromatography with ultraviolet detection

“…So, for a broad and complete determination it is advantageous for the laboratory to have methods as described here that may be used for more than a single compound. At present, determination of some of these drugs has been established by high performance liquid chromatography (HPLC) with UV detection [2,[14][15][16][17][18][19][20][21], HPLC with coulometric detection [22,23] or fluorescence detection [24,25]. Although electrochemical detection [26][27][28][29][30] offers enhanced sensitivity in the low ng/ml range, electrochemical detectors require optimal working conditions, sample preparation is critical and interference from co-medications is often unavoidable.…”