Simultaneous Determination Of Hyoscine Butyl Bromide and Dipyrone in Their Binary Mixture By RP-HPLC Method.

Abstract

RP-HPLC chromatographic method was developed for determination of Hyoscine Butyl Bromide (HBB) and Dipyrone (DIP). In this method, HBB and DIP were separated by using C18 (25 cm×4.6 mm i.d. 5µm particle size) column as a stationary phase and using water: methanol (50:50) v/v pH adjusted to 7.0 with triflouroacetic acid and triethylamine) as a mobile phase, maintaining the flow rate at 1.0 mL min −1 with UV detection at 210 nm. The proposed method was successfully applied for determination of HBB and DIP in pure form and in their pharmaceutical formulations.

Comparative Study of New Hplc And Hptlc Methods For Simultaneous Determination of Dipyrone And Camylofin Dihydrochloride Using Hyoscine Butyl Bromide As Internal Standard

“…Metamizole is a pro-drug, which spontaneously breaks down after oral administration to structurally related pyrazolone compounds [1]. Several methods have been reported for determination of dipyrone either alone or in combination with other drugs; these methods include HPLC [2][3][4][5][6], spectrophotometry [7][8][9] and Rp-TLC [10].…”