Simultaneous determination of iridoids, phenolic acids, flavonoids, and saponins in Flos Lonicerae and Flos Lonicerae Japonicae by HPLC‐DAD‐ELSD coupled with principal component analysis

Abstract

A method, HPLC coupled with diode-array and evaporative light scattering detectors (HPLC-DAD-ELSD), was newly developed to evaluate the quality of Flos Lonicerae (FL) and Flos Lonicerae Japonicae (FLJ), through a simultaneous determination of multiple types of bioactive components. By employing DAD, the detection wavelengths were set at 240 nm for the determination of iridoids, 330 nm for phenolic acids, and 360 nm for flavonoids, respectively. While ELSD, connected in series after DAD, was applied to the determination of saponins. This assay was fully validated with respect to precision, repeatability, and accuracy. Moreover, principal component analysis (PCA) was used for the similarity evaluation of different samples, and it was proven straightforward and reliable to differentiate FL and FLJ samples from different origins. For PCA, two principal components have been extracted. Principal component 1 (PC1) influences the separation between different sample sets, capturing 54.598% variance, while principal component 2 (PC2) affects differentiation within sample sets, capturing 12.579% variance. In conclusion, simultaneous quantification of bioactive components by HPLC-DAD-ELSD coupled with PCA would be a well-acceptable strategy to differentiate the sources and to comprehensively control the quality of the medicinal plants FL and FLJ.

Influence of Sulfur Fumigation on the Chemical Constituents and Antioxidant Activity of Buds of Lonicera japonica

“…As another type of pharmacologically important constituent, major iridoid glycosides have not been paid enough attention for the quality evaluation of LJF. A few investigations were reported involving in the quantitative analyses of iridoid glycosides, including sweroside, 7-epivogeloside, secoxyloganin, dimethyl-secologanoside, centauroside and loganin, along with phenolic acids and flavonoids, by HPLC-DAD-ELSD [22] and HPLC-DAD-MS [18,19,23]. However, from our study mentioned above, secologanic acid was the most abundant iridoid, and its content was far higher than those of sweroside and loganin, which were commonly used as the representatives of iridoid glycosides in LJF samples.…”

Characterization and identification of multiple constituents in Yinhuang granules by high‐performance liquid chromatography with diode‐array and time‐of‐flight mass spectrometry detection

“…According to their chromatographic retention behavior in HPLC reported before, the saponins with sugar moieties at the C-28 position eluted easier than that with sugar moieties at the C-3 position in RP-HPLC [23]. Thus C21 and C25 were tentatively identified as 1 ion at m/z 951.5117 in positive spectrum, and the molecular mass of C20 was 16 Da greater than that of C21 and C25, the elemental composition could be then undoubtedly assigned to C 47 H 76 O 18 (see [11,14].…”

“…Chemical and pharmacological investigations revealed that four types of secondary metabolites, including phenolic acids, iridoids, saponins and flavonoids, are responsible for the overall therapeutic effects of Flos Lonicerae [10][11][12][13][14]. Flavonoids such as baicalein, baicalin, wogonin and wogonoside are the major active components in Radix Scutellariae [15].…”

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

“…In recent years, an on-line coupled HPLC-DAD-ELSD method has attracted ever-increasing attention and has been successfully applied to simultaneous quantification of multi-components in traditional Chinese herbal medicines and their preparations [14][15][16][17][18][19][20][21][22][23]. This method is potentially ideal for routine analysis and quality evaluation of YST since UV and ELSD signals contain complementary information for each other.…”