Simultaneous determination of isoniazid, rifampicin, levofloxacin in mouse tissues and plasma by high performance liquid chromatography–tandem mass spectrometry

Fang, Pingfei; Cai, Hualin; Huan-de, LI; Zhu, Rong; Tan, Qin-You; Gao, Wei; Xu, Ping; Liu, Yiping; Zhang, Wenyuan; Chen, Yongchang; Zhang, Feng

doi:10.1016/j.jchromb.2010.06.038

Cited by 72 publications

(42 citation statements)

References 13 publications

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“…Intra and inter-day variability's (RSD) for extraction of INH from plasma and other tissue homogenates were less than 5% and accuracy was within ± 5% (Table 1) and were comparable with other reported methods of isoniazid [15,16,23].The absolute recovery of INH was calculated for replicate spiked QC samples (200, 1000 and10,000 ng/ mL) ( …”

Section: Stabilitysupporting

confidence: 75%

A Sensitive HPLC Method for Determination of Isoniazid in Rat Plasma, Brain, Liver and Kidney

Bh¹,

ari²,

Kaur³

2012

J Chromat Separation Techniq

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A simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of Isoniazid (INH) in plasma, brain, liver and kidney samples and in solid lipid nanoparticles (SLNs). Isoniazid was analyzed by using a reverse phase column (Waters, Symmetry shield RP-18, 4.6 mm x 150 cm, 5 microns), with mobile phase consisting of 0.1 M phosphate buffer, pH 5 (pH adjusted with ortho phosphoric acid) and methanol and the detection was made at 254 nm using Photo Diode Array detector at a temperature of 30°C (sample 4°C). The retention time for INH was around 3.5 minutes. The calibration curves were linear (r 2 0.9998) over a concentration range from 250 ng to 25,000 ng/mL. Limit of detection (LOD) was 150 ng/mL and the Limit of quantitation (LOQ) was 200 ng/mL for plasma and tissue homogenates (brain, liver and kidney). Intra and inter-day variability's (RSD) for extraction of INH from plasma and other tissue homogenates were less than 5% and accuracy was ± 5%. The results established selectivity and suitability of the method for pharmacokinetic studies of INH from INH SLNs.

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Section: Stabilitysupporting

confidence: 75%

A Sensitive HPLC Method for Determination of Isoniazid in Rat Plasma, Brain, Liver and Kidney

Bh¹,

ari²,

Kaur³

2012

J Chromat Separation Techniq

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“…The homogenization procedure also contributes to the extraction of the analytes from the tissue into a solvent that can be further treated by any of the techniques mentioned above [57,84,113]. In some cases the homogenization was carried out with a buffer, namely phosphate-buffered saline solution, but in other cases it was used in combination with a chemical disrupting reagent such as an organic solvent or an acid [84,106,108]. For the quantification of LEV from infected tissues within Teflon catheter segments, Bao et al [124] found that 5 min of vortexing was sufficient to give a homogeneous tissue suspension owing to the relatively soft matrix of the infected tissue inside the catheters subcutaneously implanted.…”

Section: Sample Preparationmentioning

confidence: 99%

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

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“…In the previous analytical methods [6][7][8][9][10][11][12][13][14][15][16][17], robustness study was not performed. However, in this method, robustness study was conducted to evaluate whether deliberate small changes in HPLC system could or couldn't influence analytical results.…”

Section: Robustnessmentioning

confidence: 99%

“…A validated bioanalytical method for quantitative estimation of LEV in human plasma at a pharmacokinetic range approximately 1-20 µg/mL is necessary to conduct the bioequivalence study [5]. A number of analytical methods for quantifying LEV were developed such as highperformance liquid chromatography coupled with ultraviolet (HPLC-UV), fluorescent (HPLC-FL), and tandem mass spectrometry (HPLC-MS/MS) detector [6][7][8][9][10][11][12][13][14][15][16][17]. Most of these method used many kinds of additives as mobile phase component for improving peak shape and resolution i.e.…”

Section: Introductionmentioning

confidence: 99%

“…triethylamine [7,[12][13][14], bromide [11], tetramethyl ammonium bromide [8], butadiene styrene brominated ammonium [9], tetrabutylammonium hydrogen sulfate [10], and trifluoroacetic acid [6,15]. The other method was developed under gradient elution using a formic acid 0.05% and methanol as mobile phase component [17], but this method gives relatively long separation time (about 13 min). Use of additive for improving separation may have disadvantages including slow equilibrium time and reduce column lifetime [18].…”

Section: Introductionmentioning

confidence: 99%

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A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

et al. 2017

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Simultaneous determination of isoniazid, rifampicin, levofloxacin in mouse tissues and plasma by high performance liquid chromatography–tandem mass spectrometry

Cited by 72 publications

References 13 publications

A Sensitive HPLC Method for Determination of Isoniazid in Rat Plasma, Brain, Liver and Kidney

A Sensitive HPLC Method for Determination of Isoniazid in Rat Plasma, Brain, Liver and Kidney

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

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