Simultaneous determination of levodopa and carbidopa by synchronous fluorescence spectrometry using double scans

Kim, Wook Hyun; Karim, Mohammad Mainul; Lee, Sang Hak

doi:10.1016/j.aca.2008.01.006

Cited by 48 publications

(17 citation statements)

References 31 publications

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“…Moreover, the D which gives the highest synchronous fluorescence intensity for a particular species is chosen as the optimum D and the corresponding peak is taken as the optimum peak. Therefore, a suitable D value is needed for the accurate determination of each component in a complex sample [19,22]. The selection of this parameter was usually made empirically to achieve maximum selectivity for each component signals in a mixture.…”

Section: Selection Of Optimum Dmentioning

confidence: 99%

“…Fortunately, the selectivity problem resulting from the overlapping of the broad-band spectra of PAHs metabolites can be solved by using synchronous fluorescence spectrometry (SFS) method [17]. It has been known as a powerful tool for simultaneous analysis of multicomponent samples without any pretreatment of samples [18,19]. In SFS both excitation and emission monochromators are scanned simultaneously, maintaining a constant wavelength interval between them.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of α-naphthol, β-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using β-cyclodextrin as a sensitiser

Wang

Hong-mei

et al. 2011

International Journal of Environmental Analytical Chemistry

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Section: Selection Of Optimum Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of α-naphthol, β-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using β-cyclodextrin as a sensitiser

Wang

Hong-mei

et al. 2011

International Journal of Environmental Analytical Chemistry

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“…These are commonly carried out by potentiometry [3], voltammetry [4,5], high performance liquid chromatography (HPLC) [6][7][8], capillary electrophoresis (CE) [9,10], NMR 1 H [11], fluorescence [12], synchronous fluorescence [13] and UV-vis spectrophotometry [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Kinetic approach for the enzymatic determination of levodopa and carbidopa assisted by multivariate curve resolution-alternating least squares

Grünhut¹,

Garrido²,

Centurión³

et al. 2010

Analytica Chimica Acta

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“…On resolving a mixture of fluorescent components, fluorescent scanning is greatly beneficial in simplifying the spectra and decreasing the extent of spectral overlaps. [21][22][23][24] To entirely separate synchronous fluorescence peaks of ATV and EZE, the synchronous fluorescence spectra of the mixture were recorded using a wide range of different Δλ values (20-100 nm). The best resolution of the mixture was achieved when the Δλ of 100 nm for ATV and that of 40 nm for EZE were selected.…”

Section: Optimization Of Experimental Variablesmentioning

confidence: 99%

Application of New Spectrofluorometric Techniques for Determination of Atorvastatin and Ezetimibe in Combined Tablet Dosage Form

Ayad

Magdy

2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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Two accurate, reliable, and highly sensitive spectrofluorometric methods were developed for simultaneous determination of the binary mixture of Atorvastatin and Ezetimibe without prior separation steps. The first method is based on double scan synchronous fluorescence spectrometry. Each of Atorvastatin and Ezetimibe can be determined independent of the other when scanned at Δλ 100 nm and 40 nm, respectively. The relative fluorescence intensity-concentration plots at two wavelengths, 272 (Δλ 100 nm) and 266 nm (Δλ 40 nm) were rectilinear over the range of 0.4-8 µg/mL (for Atorvastatin) and 0.6-8 µg/mL (for Ezetimibe), respectively. The second method is based on the technique of simultaneous equations (Vierodt's method), in which two equations are solved simultaneously after using a single excitation wavelength of 273 nm and λ Em1 380 nm of Atorvastatin and λ Em2 301 nm of Ezetimibe. Under the optimum conditions, linear relationships were found between the relative fluorescence intensity and the concentrations of the investigated drugs in the range of 0.4-8 µg/mL (for Atorvastatin) 0.6-8 µg/mL (for Ezetimibe). The different experimental parameters affecting the fluorescence intensities of the two drugs were carefully studied and optimized. The proposed methods were successfully applied for the determination of the investigated drugs in pure form, dosage form and in synthetic mixtures with good recovery and the results obtained were favorably compared to those obtained with a reference method.Key words Atorvastatin; Ezetimibe; synchronous fluorescence spectrometry; Vierodt's method Atorvastatin calcium (ATV), a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, is a lipid regulating drug used to reduce low density lipoprotein (LDL)-cholesterol, apolipoprotein B and triglycerides, and to increase high density lipoprotein (HDL)-cholesterol in the treatment of hyperlipidaemias (Fig. 1a). It is also used for prophylaxis of cardiovascular events in patients with multiple risk factors including diabetes mellitus. ATV is a member of the drug class known as statins. 1)Ezetimibe (EZE) is an inhibitor of intestinal absorption of cholesterol and plant sterols (Fig. 1b). It prevents transport of cholesterol through the intestinal wall by selectively blocking the absorption of cholesterol from dietary and biliary sources. This reduces the overall delivery of cholesterol to the liver, thereby promoting the synthesis of LDL receptors and a subsequent reduction in the serum LDL-C.2) Combining the different mechanisms of action of these agents appears to provide substantial reductions in LDL-C, with additional favorable changes in total cholesterol, triglycerides, and HDL-C. Clinical studies have shown that co-administration of EZE plus ATV was significantly more effective at reducing LDL-C concentrations than EZE or ATV alone.3) There is an urgent need to develop analytical methods for the simultaneous analysis of EZE and ATV in pharmaceutical dosage forms due to the continuous increase in clinical use of these t...

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Simultaneous determination of levodopa and carbidopa by synchronous fluorescence spectrometry using double scans

Cited by 48 publications

References 31 publications

Simultaneous determination of α-naphthol, β-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using β-cyclodextrin as a sensitiser

Simultaneous determination of α-naphthol, β-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using β-cyclodextrin as a sensitiser

Kinetic approach for the enzymatic determination of levodopa and carbidopa assisted by multivariate curve resolution-alternating least squares

Application of New Spectrofluorometric Techniques for Determination of Atorvastatin and Ezetimibe in Combined Tablet Dosage Form

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