Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching

Nguyen, Hoang Anh; Grellet, Jean; Ba, Boubakar B.; Quentin, Claudine; Saux, Marie-Claude

doi:10.1016/j.jchromb.2004.07.019

Cited by 45 publications

(40 citation statements)

References 22 publications

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“…The stock solutions of the new fluoroquinolones, used for the validation of liquid chromatographic methods, have been prepared in several solvents: water, methanol, acetonitrile and a mixture of water/methanol or water/acetonitrile [54][55][56][57][58]. In some cases, to deviate from neutral pH and thus enhance solubility in water, a basic solution (NaOH) was added in the preparation of LEV and SPA stock solutions, whereas for GAT, MOX, TRO and ULI an acid (HCl, formic acid, orthophophoric acid) or a phosphate buffer at pH 3 were also used [59][60][61][62][63][64][65].…”

Section: Physicochemical Properties and Stability Of Fluoroquinolonesmentioning

confidence: 99%

“…Technological advances have permitted the integration of sample preparation and chromatographic separation into a single system leading to fully automated on-line SPE. This labour-saving procedure has been used for direct injection of plasma samples [55,94]. For this purpose two types of on-line SPE columns were developed, namely restricted access media (RAM) and turbulent flow chromatography (TFC) columns [82,99].…”

Section: Sample Preparationmentioning

confidence: 99%

“…In order to optimize the chromatographic separation of LEV, GAT and MOX, Nguyen et al [55] chose a mobile phase at pH 2.5 to reverse fluoroquinolone carboxylic function ionization; they evaluated the influence of pH in the range 2.0-4.0 and concluded that the resolution of fluoroquinolones did not vary significantly within this range. Ocaña González et al [67] investigated the retention time of GRE using acetonitrile/0.1 M phosphoric acid-sodium hydroxide buffer/0.01 M n-octylamine as mobile phase over the pH range 2.5-6.0 and found that it remained almost constant in the range 2.5-3.5, whereas it increases approximately 50% at pH 6.0; therefore, the value of pH 3.0 was selected for the mobile phase.…”

Section: Chromatographic Separationmentioning

confidence: 99%

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Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

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Section: Physicochemical Properties and Stability Of Fluoroquinolonesmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Chromatographic Separationmentioning

confidence: 99%

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Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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“…Simultaneous determination methods of H 2 receptor antagonists with enoxacin, and cefixime have been developed by Arayne et al, (2007Arayne et al, ( , 2008. Nguyen et al, (2004) reported simultaneous determination of levofloxacin, gatifloxacin, and moxifloxacin in human serum. Nemutlue et al, (Nemutlu et al, 2007) published simultaneous separation and determination of seven quinolones using HPLC and analysis of levofloxacin.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sparfloxacin and commonly used H2 receptor antagonists by RP-HPLC: application to in vitro drug interactions

Sultana¹,

Arayne²,

Gul

et al. 2011

Med Chem Res

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Innovative, simple, economical, and rapid reverse phase HPLC method has been developed and validated for simultaneous determination of sparfloxacin (SPFX) and cimetidine, ranitidine, and famotidine (H 2 receptor antagonists) in bulk material, pharmaceutical formulations, and human serum. Chromatographic system was consisting of schemed HPLC system having UV detector set at 232 nm for cimetidine and, 250 nm for famotidine and ranitidine. Purospher STAR C 18 (250 9 4.6 mm, 5 lm) column was used, and the mobile phase (methanol, water and ACN 54:41:5 v/v/v pH 2.7 adjusted by phosphoric acid) was delivered at a flow rate of 1.0 ml min -1 . The proposed method is specific, accurate (98.02-102.136%), precise (intra-day and inter-day variation 0.501-2.179%) and linear (R 2 [ 0.999) with in the desired range 0.3125-25 lg ml -1 concentration. The detection limit and quantification limit were 0.009-0.084 and 0.030-0.255 lg ml -1 , respectively. Paired t test was applied to verify the results. The anticipated method is applicable to routine analysis of SPFX and H 2 receptor antagonists in pharmaceutical formulations as well as in human serum samples. The proposed method is also applied to study interaction studies of SPFX with H 2 receptor antagonists.

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confidence: 99%

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

Ashour¹,

Kattan²

2016

SOJPPS

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Research articleperformance liquid chromatography with fluorescence [29,30] or UV detection [31][32][33][34][35], liquid chromatography tandem mass spectrometry (LC/MS/MS) [36,37] and capillary electrophoresis [38,39] are also reported for determination of moxifloxacin hydrochloride from human body fluids. British Pharmacopoeia (BP) [40] employs HPLC method for the assay of moxifloxacin hydrochloride in pharmaceutical preparations. Further, the BP describes no environmentally friendly chromatographic procedure since acetonitrile is one of the components of mobile phase.This work describes a rapid and sensitive RP-HPLC method with UV detection for determination of moxifloxacin hydrochloride in bulk drug and tablet formulation with low cost of mobile phase, which was used for the first time in this work. This method serves as an alternative to the methods described in pharmacopoeias and can be conveniently used for routine quality control analysis of moxifloxacin. Experimental MaterialsHPLC grade methanol (Merck, Germany), formic acid (AR grade, Surechem Products LTD, England) and water (HPLC grade, Merck). Moxifloxacin hydrochloride (MOXF) pure drug substance, its purity was found to be 99.7%, and rosuvastatin calcium (ROSV) was obtained from Matrix Laboratories Limited and MSN Laboratories Limited (India), respectively. Tablets were purchased from Syrian market, containing moxifloxacin hydrochloride 400 mg per tablet. AbstractSimple, rapid, sensitive and accurate method for determination of moxifloxacin HCl (MOXF) in bulk drug and tablet formulation was developed and validated by using HPLC. Rosuvastatin calcium (ROSV) was used as internal standard. Chromatography was performed with mobile phase consisted of 0.1 M formic acid (pH 2.80) and methanol (30:70, v/v), with a flow rate of 1.0 mL/min, Nucleodur C18 column (250 mm×4.6 mm i.d., 5 m particle size) and photodiode array (PDA) detection at 280 nm. The method was validated for linearity, specificity, accuracy, precision, stability and robustness. Linearity range was 1.12-590.0 µg/mL with limit of detection value of 0.23 µg/mL. The precision of the method was demonstrated using intraand inter-day assay RSD% values which were less than 2%, while the recovery was 99.11-103.85%. The developed method was very rapid with a run time of 3 min and found to be successively applied for the quality control of MOXF in bulk drug and tablets.

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Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching

Cited by 45 publications

References 22 publications

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Simultaneous determination of sparfloxacin and commonly used H2 receptor antagonists by RP-HPLC: application to in vitro drug interactions

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

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