Simultaneous determination of sparfloxacin and commonly used H2 receptor antagonists by RP-HPLC: application to in vitro drug interactions

Sultana, Najma; Arayne, M. Saeed; Gul, Somia; Akhtar, Mahwish; Shamim, Sana

doi:10.1007/s00044-011-9586-4

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Accuracy and precision of the method were found in acceptable boundary 98.02-102.136% and 0.5-2.2%, respectively. Statistical Paired -test was applied for the verification of the collected data [216]. HPLC simultaneous determination of CIM, RAN, FAM, and NIZ was established using a two-level, full-factorial design with three variables (volume of methanol, percentage of triethylamine, and concentration of phosphate buffer).…”

Section: Simultaneous Determination Of H 2 Receptor Antagonistsmentioning

confidence: 99%

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Shafi

Siddiqui

Naseem

et al. 2013

Journal of Chemistry

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This review article recapitulates the analytical methods for the quantitative determinations of diltiazem and three H2receptor antagonists (cimetidine, ranitidine, and famotidine) by one of the spectroscopic technique (UV spectrophotometery) and separation technique such as high-performance liquid chromatography (HPLC). The clinical and pharmaceutical analysis of these drugs requires effective analytical procedures for quality control, pharmaceutical dosage formulations, and biological fluids. An extensive survey of the literature published in various analytical and pharmaceutical chemistry-related journals has been compiled in its review. A synopsis of reported spectrophotometric and high-performance liquid chromatographic methods for individual drug is integrated. This appraisal illustrates that majority of the HPLC methods reviewed are based on the quantitative analysis of drugs in biological fluids, and they are appropriate for therapeutic drug monitoring purpose.

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Section: Simultaneous Determination Of H 2 Receptor Antagonistsmentioning

confidence: 99%

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Shafi

Siddiqui

Naseem

et al. 2013

Journal of Chemistry

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“…Many analytical methods have been developed to determine statins, specifically rosuvastatin alone, such as liquid chromatography (Pasha et al ., ), or in combination with other drugs over the past decade as with simvastatin and pravastatin sodium (Chaudhari et al ., ), atorvastatin, lovastatin, pravastatin and simvastatin (Pasha et al ., ), with finofibrate (Sharma and Bhandari, ), with atenolol, spironolactone, glibenclamide and naproxen sodium (Sultana et al ., ), with pioglitazone, gliquidone and simvastatin, with simvastatin, atorvastatin, pravastatin and ceftriaxone (Arayne et al ., ), with diltiazem, atorvastatin and simvastatin (Sultana et al ., ), with lisinopril, pravastatin and atorvastatin (Sultana et al ., ), with captopril, atorvastatin and simvastatin (Sultana et al ., ), with Angiotensin Converting Enzyme (ACE) inhibitors (Arayne et al ., ) and with Non Steroidal Antiinflammatory Drugs (NSAIDs) (Arayne et al ., ; Sultana et al ., ). A number of spectrophotometric and RP‐HPLC methods have been reported for the quantitative determination of fluoroquinolones in pharmaceutical formulations and spiked human serum (Joshi, ; Belal et al ., ; Santoro et al ., ; Arayne et al ., ; Sultana et al ., ) as well as simultaneous determination of quinolones with other co‐administered drugs (Gul et al ., ; Sultana et al ., ,,, ).…”

Section: Introductionmentioning

confidence: 99%

Facile LC‐UV methods for simultaneous monitoring of ciprofloxacin and rosuvastatin in API, formulations and human serum

Arayne

Sultana

Tabassum

2014

Biomedical Chromatography

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An efficient, selective and cost-effective liquid chromatographic assay was developed and validated for the simultaneous quantification of ciprofloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. The chromatographic system consisted of mobile phase methanol-water, 90:10 v/v at pH 3.0 adjusted with o-phosphoric acid, pumped at 1.0 mL/min through a prepacked Purospher Star C18 (5 µm, 25 × 0.46 cm) column and effluent was monitored at the isosbestic point (255 nm) as well as at the λmax of individual drugs (243 and 271 nm). The method was validated over a linear concentration range of 0.25-15 µg/mL for ciprofloxacin and 0.33-20 µg/mL for rosuvastatin (r(2) ≥ 0.999). The ranges of reliable response (limits of detection and quantitation) for ciprofloxacin were 3-15 and 9-45 ng/mL and 17-29 and 52-88 ng/mL, respectively, for rosuvastatin in all API, pharmaceutical formulations and human serum. Analytical recovery from human serum was >98% and relative standard deviation (RSD) was <2. The accuracies were 97.13-102.55 and 97.41-101.31% and precisions in RSD were 0.04-1.90 and 0.02-1.23% for ciprofloxacin and rosuvastatin, respectively. No matrix interferences, ion suppression/enhancement and carry-over were detected. The total assay run time was less than 5 min. In another study, for optimum performance the detector was programmed for multiwavelength scanning at the absorption maxima of each component. Consequently, the linearity range was improved and limit of detection and quantitation values were down to 1-4 and 4-12 ng/mL for ciprofloxacin and 3-5 and 9-15 ng/mL for rosuvastatin, respectively. The validation parameters fitted ICH guidelines through the isosbestic and individual λmax approach. The small sample volume and simplicity of preparation make this method suitable for use in human serum samples, pharmaceutical formulations, quality control, drug-drug interaction studies, clinical laboratories, drug research centers and forensic medical centers.

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“…A number of methods for the simultaneous determination of coadminstered drugs have also been reported by our research group as simultaneous determination of rosuvastatin, lisinopril, captopril and enalapril [19], lisinopril, [20] verapamil [21], captopril [22], ceftriaxone sodium [23], diltiazem [24] rosuvastatin [25] and sparfloxacin [26] with NSAIDs in API, pharmaceutical formulations and human serum by RP-HPLC. Arayne et al [27][28][29][30][31][32][33][34] and Sultana et al [35][36][37][38][39][40] have previously reported, a number of methods for drug-drug interaction studies using UV-visible spectroscopic and RP-HPLC. In continuation of this work we have attempted to study in vitro interactions of leflunomide with flurbiprofen, ibuprofen, diclofenac sodium and mefenamic acid (Figure 1), the experiment was accomplished by the use of UV-visible spectroscopic and RP-HPLC techniques.…”

Section: Introductionmentioning

confidence: 99%

Development of Liquid Chromatography? UV Method for Simultaneous Determination of Leflunomide and NSAIDs in API and Pharmaceutical Formulations: It?s Application to In vitro Interaction Studies

Sultana¹,

Arayne²

2012

Med Chem (Los Angeles)

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An efficient analytical method for the simultaneous determination of leflunomide and non steroidal anti-inflammatory drugs in API and formulations by LC-UV has been developed. The analytes were separated on Purospher Star, C 18 (5 µm, 250×4.6 mm) column at ambient temperature with methanol: water (80:20, v/v, pH at 2.7) at flow rate of 1.5 mL min -1 . Experiment was conducted in two phases. Leflunomide was separated with flurbiprofen and ibuprofen (phase-I) and diclofenac sodium and mefenamic acid (phase II). Calibration curves were linear over the range 0.625-5 µg mL -1 in both phases for leflunomide while for flurbiprofen, ibuprofen, diclofenac sodium, mefenamic acid linearity were achieved in the range of 0.625-5, 11.25-90, 1.56-50 and 0.78-25 µg mL -1 , respectively with r 2 >0.9998. Intraday variation was <1.2 and <1.4 %, while in inter-day ranged between 0.042-1.45% and 0.08-1.27% in phase-I and II, respectively. Mean recovery values for intra-day ranged from 99.04-100.4% and 98.48-100.2% and for inter-day were between 98.54-100.29% and 98.85-100.54% in phase-I and II, respectively. The LLOD of leflunomide was 13 ng mL -1 , while LLOQ was 39ng mL -1 , respectively. LLOD and LLOQ for flurbiprofen, ibuprofen, diclofenac sodium and mefenamic acid were 6.9, 296, 71 and 1.2 ng mL -1 and 21, 897, 214.3 and 3.676 ng mL -1 , respectively. Present study showed that nanogram quantities of all the compounds can be estimated accurately. The newly established method was successfully applied to study in vitro interactions between leflunomide and NSAIDs.

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Simultaneous determination of sparfloxacin and commonly used H2 receptor antagonists by RP-HPLC: application to in vitro drug interactions

Cited by 6 publications

References 20 publications

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC Technique

Facile LC‐UV methods for simultaneous monitoring of ciprofloxacin and rosuvastatin in API, formulations and human serum

Development of Liquid Chromatography? UV Method for Simultaneous Determination of Leflunomide and NSAIDs in API and Pharmaceutical Formulations: It?s Application to In vitro Interaction Studies

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