Simultaneous determination of methamphetamine and amphetamine in human urine using pipette tip solid-phase extraction and gas chromatography–mass spectrometry

Kumazawa, Takeshi; Hasegawa, Chika; Lee, Xiao‐Pen; Hara, Kenji; Seno, Hiroshi; Сузукі, Осаму; Sato, Keizo

doi:10.1016/j.jpba.2006.12.025

Cited by 96 publications

(35 citation statements)

References 24 publications

(35 reference statements)

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“…Analytical methods previously reported to analyze drugs of abuse in human urine samples include GC/MS [50], high-performance liquid chromatography (HPLC) [51], capillary electrophoresis (CE) [52], and CE-MS [53], which involved time-consuming sample extraction, derivatization, or separation. In our experiments, methamphetamine-spiked raw urines were directly ionized using DESI.…”

Section: Urine Analysis-direct Monitoring Of Drugs Of Abuse In Urinementioning

confidence: 99%

Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS)

Miao

Chen

2009

J. Am. Soc. Mass Spectrom.

150

192

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Desorption electrospray ionization-mass spectrometry (DESI-MS) was evaluated for the direct analysis of liquid samples. Several interesting results were found. First, in contrast to the previous DESI analysis of dried solid samples that was limited to proteins with MW Յ 25 kDa (Anal. Chem. 2007, 79, 3514), bovine serum albumin (BSA, 66 kDa) was successfully ionized from solutions by DESI with observation of corresponding multiply charged ions. Second, direct DESI analysis of protein tryptic digest solutions without chromatographic separation, sample clean-up, and the sample drying step was demonstrated, providing reasonably good sequence coverage of 52% to 97%. Third, direct analysis of biofluids such as an undiluted urine sample without sample pretreatment is possible, emphasizing the high tolerance of DESI with salt. These results suggest that a charged droplet pick-up mechanism is responsible for desorption and ionization of liquid samples by DESI. Also, unlike in electrospray ionization (ESI), inhibition of electrochemical reduction in the negative ion mode was observed for liquid sample DESI. In addition, reactive DESI can be performed with ion/ion reactions of Zn(II) complexes for the selective binding of phosphoserine in the presence of serine. DESI experiment can also be carried out directly to liquid samples flowing out of a pumped syringe needle tip, allowing rapid analysis. Furthermore, on-line coupling of electrochemical cell with DESI-MS was demonstrated, in which perylene radical cations generated in the cell were successfully transferred to the gas-phase for MS detection by DESI. This study extended the scope of DESI-MS applications, which could have potentials in bioanalytical and forensic analysis. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] have been introduced as rapid tools to provide efficient desorption, ionization, and further mass spectrometric characterization of target compounds. As a representative ambient ionization method, desorption electrospray ionization (DESI) has become very successful in the fast analysis of a variety of different analytes including pharmaceuticals [17][18][19], metabolites [20,21], drugs of abuse [22,23], explosives [24 -26], chemical warfare agents [27], and even intact tissues [28,29] as well as thin-layer chromatography plate [30]. In the DESI experiments, ionization occurs via the interaction of charged microdroplets generated in a pneumatically assisted electrospray of an appropriate solvent with samples placed on surfaces. Typically, solid samples on the surface are desorbed and ionized during DESI-MS analysis, and liquid samples typically undergo drying on surface in air before ionization. One practical reason for this is that liquid samples could be blown away from the surface immediately (i.e., splashing of liquids) by the high-velocity nebulizing gas employed for the generation of charged microdroplets. As a consequence, the resulting ion signal, if any, will not last long. Recently, two novel sample introduction methods have been reported ...

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Section: Urine Analysis-direct Monitoring Of Drugs Of Abuse In Urinementioning

confidence: 99%

Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS)

Miao

Chen

2009

J. Am. Soc. Mass Spectrom.

150

192

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“…Analytical strategies including gas chromatography (GC) [12,13], high performance liquid chromatography (HPLC) [14,15], gas chromatography-mass spectrometry (GC-MS) [10,12,[16][17][18][19][20][21] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [22,23] have been employed. The majority of samples received by our laboratory are direct confirmation requests by clinicians.…”

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confidence: 99%

Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Marais

Laurens

2009

Forensic Science International

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“…MonoTip C18 tips containing fixed C18-bonded monolithic silica gel has been used to extract several drugs from urine and plasma samples. [77][78][79] Recently, an in-tip SPME using Omix C18 coupled with LC-MS/MS was developed for the determination of illicit drugs, such as stimulants, hallucinogens, ketamine and phencyclidine, in oral fluids. 80 Furthermore, a high-throughput in-tip SPME using 96-well pipette tips containing a chemically bonded monolithic methacrylate sorbent was developed for drug analyses in plasma (Sect.…”

Section: ·3 In-tube Spme Techniquesmentioning

confidence: 99%

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

Kataoka

2011

ANAL. SCI.

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Simultaneous determination of methamphetamine and amphetamine in human urine using pipette tip solid-phase extraction and gas chromatography–mass spectrometry

Cited by 96 publications

References 24 publications

Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS)

Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS)

Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

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