Simultaneous determination of moxifloxacin and ofloxacin in physiological fluids using high performance liquid chromatography with ultraviolet detection

Khan, Fahim Ullah; Nasir, Fazli; Iqbal, Zafar; Khan, Ismail; Shahbaz, Naila; Hassan, Muhammad; Ullah, Farhad

doi:10.1016/j.jchromb.2016.03.002

Cited by 34 publications

(18 citation statements)

References 23 publications

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“…By gradually increasing amount of moxifloxacin in human plasma sample, the determined LOD and LOQ values were 0.15 and 0.50 µg/mL, respectively (Table 1). There are also lower levels of quantification for HPLC-UV method reported [8,9,11], however in clinical practice…”

Section: Lod and Loqmentioning

confidence: 99%

“…On the other side, HPLC with UV detector was employed for applications in routine therapeutic drug monitoring or clinical pharmacokinetic studies of moxifloxacin. Most reported HPLC-UV methods involve a sample pretreatment step in which methanol [8], acetonitrile [9,10] or dichloromethane [11] was used as precipitating solvent. Alternatively, in this paper, we report a simple HPLC-UV method for determination of moxifloxacin in plasma using trichloroacetic acid within deproteinization step.…”

Section: Moxifloxacinmentioning

confidence: 99%

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High Performance Liquid Chromatography Assay for Determination of Moxifloxacin in Human Plasma

Hanh

Diep²,

Thị

et al. 2019

JST

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A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with trichloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25 cm × 4.5 mm, 5 µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (30:70, v/v) as mobile phase at a flow rate of 0.8 mL/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30 %. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.

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Section: Lod and Loqmentioning

confidence: 99%

Section: Moxifloxacinmentioning

confidence: 99%

High Performance Liquid Chromatography Assay for Determination of Moxifloxacin in Human Plasma

Hanh

Diep²,

Thị

et al. 2019

JST

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“…[5][6][7] Hence, diclofenac sodium and moxifloxacin ophthalmic solutions are used simultaneously after the cataract surgery. Several analytical methods such as spectrophotometry, [8][9][10][11][12] RP-HPLC [13][14][15][16][17][18][19][20][21][22][23] and electroanalytical methods [24][25][26] were reported in the literature for the quantification of DCL and MOX alone or with other drugs. However, the simultaneous determination of DCL and MOX has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation

Chohan¹,

Elgorashe²,

Balgoname³

et al. 2019

IJPER

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Background: Diclofenac Sodium (DCL) and Moxifloxacin HCl (MOX) were simultaneously used after cataract surgery to reduce the post-operative inflammation and to control infection respectively. Objectives: Three simple, accurate, eco-friendly and reproducible UV spectroscopic methods were established for concurrent determination of diclofenac sodium and moxifloxacin in ophthalmic preparation without prior separation. Methods: The first technique was established on the measurement of a peak amplitude of the first derivative spectra at the zero-crossing wavelength of one analyte. The second method was the determination of peak amplitude difference between peak and trough of ratio spectra. The third method involves the measurement of the peak amplitude of the first derivative of ratio spectra. Water has been used as a solvent. Results: The analytes exhibited good linearity in the range of 1-15 µg/mL for DCL and 1-18 µg/mL for MOX with excellent correlation coefficient (r 2 >0.999). Low percent relative standard deviation confirmed the precision of the methods. Excellent recovery with low percent relative error proved the accuracy of the methods. The specificity of the methods was evaluated by analyzing the laboratory prepared solutions of DCL and MOX. Conclusion: Proposed three techniques were effectively utilized for the simultaneous determination of DCL and MOX from ophthalmic preparation. The outcomes of the proposed procedures were compared with the earlier described methods and no statistical difference was found between the methods in terms of accuracy and precision.

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“…Up to now, various techniques such as fluorimetric detection , UV visible spectrophotometry , high performance liquid chromatography (HPLC) , liquid chromatography‐mass spectrometry (LC‐MS) , capillary electrophoresis and microbiological assay have been developed for the determination of OFL. However, these methods have exhibited some drawbacks such as time‐consuming in sample detection, complex and expensive instruments required, and complicated sample pretreatments needed.…”

Section: Introductionmentioning

confidence: 99%

Differential Pulse Voltammetry Determination of Ofloxacin in Human Serum and Urine Based on a Novel Tryptophan‐graphene Oxide‐carbon Nanotube Electrochemical Sensor

Zhu

Chen

et al. 2019

Electroanalysis

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The highly sensitive determination of ofloxacin (OFL) in human serum and urine was achieved on a novel tryptophan‐graphene oxide‐carbon nanotube (Trp‐GO‐CNT) composite modified glassy carbon electrode (Trp‐GO‐CNT/GCE). The Trp‐GO‐CNT composite was fabricated, and its morphologies and surface functional groups were characterized by field emission scanning electron microscopy (FE‐SEM) and Fourier transform infrared (FT‐IR) spectroscopy. The electrochemical properties of Trp‐GO‐CNT/GCE were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The superior electrochemical behaviors of Trp‐GO‐CNT/GCE toward OFL can be mainly assigned to the excellent electrocatalytic activity of Trp, the great conductivity and high surface area of GO and CNT, and the synergistic effect between Trp, GO and CNT. Under optimum conditions, a wide and valuable linear range (0.01–100 μM), a low detection limit (0.001 μM, S/N=3), a good linear relationship (R2>0.999), good stability and repeatability were obtained for the quantitative determination of OFL. Furthermore, the Trp‐GO‐CNT electrochemical sensor was successfully applied to the determination of OFL in human serum and urine samples, and satisfactory accuracy and recovery could be obtained.

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Simultaneous determination of moxifloxacin and ofloxacin in physiological fluids using high performance liquid chromatography with ultraviolet detection

Cited by 34 publications

References 23 publications

High Performance Liquid Chromatography Assay for Determination of Moxifloxacin in Human Plasma

High Performance Liquid Chromatography Assay for Determination of Moxifloxacin in Human Plasma

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation

Differential Pulse Voltammetry Determination of Ofloxacin in Human Serum and Urine Based on a Novel Tryptophan‐graphene Oxide‐carbon Nanotube Electrochemical Sensor

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