Simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma by UPLC‐MS/MS and its application to a pharmacokinetic study after administration of Reduning injection

Abstract

A simple, specific and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established and validated for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and geniposide in rat plasma using puerarin as an internal standard (IS). Plasma samples were pretreated by a one-step direct protein precipitation procedure with acetonitrile after acidified using as little as 50 μL plasma. Chromatographic separation was performed on an Acquity BEH C18 column (100 × 2.1 mm, 1.7 µm) at a flow rate of 0.2 mL/min by a gradient elution, using 0.2% acetic acid-methanol as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via electrospray ionization source with negative ion mode. Calibration curves showed good linearity (r > 0.995) over wide concentration ranges. The intra- and inter-day precisions were <15%, and the accuracy was within ±8.0%. The validated method was successfully applied to a pharmacokinetic study of the four bioactive components in rats after intravenous administration of Reduning injection.

Contribution of phenolic acids isolated from green and roasted boiled-type coffee brews to total coffee antioxidant capacity

“…Identification and 119.22 ± 2.66 e quantitation of phenolic acids was carried out by comparison of the obtained retention time peaks of the examined samples with the retention time of the corresponding standards and by using obtained calibration curves for each standard. The 3-and 4-caffeoylquinic acids were collected in the end of the HPLC capillary and identified by mass spectrometer as was described by Wang et al [35]. The 3-and 4-caffeoylquinic acids and other unidentified phenolic acids were quantified as 5-caffeoylquinic acid (chlorogenic acid).…”

A sensitive LC–MS/MS method for simultaneous quantification of geniposide and its active metabolite genipin in rat plasma and its application to a pharmacokinetic study

“…Various chromatographic methods have been published for the determination of GE or GP in combination with other compounds in the biological matrix using HPLC‐UV or LC–MS/MS (Chen et al, ; Cheng, Lin, Lin, & Tsai, ; He et al, ; Hu et al, ; Huang et al, ; Peng et al, ; Qu et al, , ; Wang et al, ). Some methods have been established for simultaneous quantification of GE and GP.…”

Insight into the molecular mechanism of a herbal injection by integrating network pharmacology and in vitro

“…The underlying mechanism is normally not clear . It was reported that polyphenol compounds and iridoids were the main components in RDN, including Chlorogenic Acid, Caffeic Acid, Isochlorogenic Acid B, Isochlorogenic Acid A, Isochlorogenic Acid C,Geniposide and so on (Wang et al, 2014;Zhang et al, 2014). Recent studies demonstrated that Chlorogenic Acid, Isochlorogenic Acid A, Isochlorogenic Acid B and Isochlorogenic Acid C could attenuate the release of PGE 2 and IL-6 in macrophage Shan et al, 2009).…”