Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

See, Khoo Lay; Elbashir, Abdalla A.; Saad, Bahruddin; Ali, Abdussalam Salhin Mohamed; Aboul‐Enein, Hassan Y.

doi:10.1002/bmc.1251

Cited by 38 publications

(16 citation statements)

References 22 publications

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“…Different techniques have been reported for the determination of Levofloxacin by Spectrophotometry [9][10][11], High Performance liquid chromatography [12], Capillary zone electrophoresis [13], Ligand exchange chromatography [14] and Nuclear magnetic resonance spectroscopy [15].…”

Section: Structure Of Levofloxacinmentioning

confidence: 99%

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

Mittal¹

2016

JAPLR

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Levofloxacin (LFX), an antimicrobial agent has been detected by using picric acid (PA) as an anionic reagent in aqueous medium. On mixing the reactants in distilled water yellow colored product is separated within two minutes which was found to be an ionassociation complex (LFX-PA) of both the reactants. Spectroscopic and voltammetric studies were carried out to explain the interaction between levofloxacin and picric acid. Spectroscopic study of the ion-pair product confirmed its formation as determined from substantial shift in respective λ max values. In voltammetric experiments, a shift of 70 mV was noticed in an anodic peak of LFX-PA (1.64V) accompanied with a large decrease in peak current. In addition, a number of new peaks appeared in voltammogram of LFX-PA. Similarly, in case of cathodic cycle, new peaks appeared in differential pulse voltammogram of the ion-pair which proved the interaction of the PA and LFX. A calibration curve was drawn using the peak current values of the LFX. The linear increase in peak current with increasing LFX concentration was obtained in the concentration range 1.5×10-3 M -6.0×10 -3 M with R 2 = 0.994. A study on the solvent effect on the spectrometric as well as voltammetric methods for LFX suggested that water is the best medium for the study. Stability of the complex was also determined by computational methods using DFT/B3LYP/6-31G basis sets. Further, analysis of LFX in commercially available drug tablets proved that the proposed method is valid in real life sample analysis.

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Section: Structure Of Levofloxacinmentioning

confidence: 99%

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

Mittal¹

2016

JAPLR

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“…Buffer acidity may affect mobility and EOF by changing the dissociation constant of analyte and silanol groups on the capillary [18]. Therefore, the first step in method development was to choose a suitable type and pH of the BGE.…”

Section: Optimization Of Electrophoretic Conditionsmentioning

confidence: 99%

Stress degradation studies on aliskiren and the development of a sensitive stability-indicating MEKC method

Sangoi

Wrasse-Sangoi

Hurtado

et al. 2013

Acta Chromatographica

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Summary.A sensitive, fast, and stability-indicating micellar electrokinetic chromatographic (MEKC) method was developed and validated for the analysis of aliskiren (ALI) in tablets using nimesulide as internal standard (IS). Optimal conditions for the separation of ALI and its degradation products were investigated. The method employed 60 mM Tris buffer and 50 mM anionic detergent sodium dodecyl sulfate (SDS) solution at pH 9.8. MEKC method was performed on a fused-silica capillary (50 μm id; effective length, 40 cm). The capillary temperature was maintained at 35°C, and the applied voltage was 30 kV, with detection by photodiode array (PDA) detector set at 204 nm. The method was validated in accordance with the International Conference on Harmonisation (ICH) requirements. The stability-indicating capability of the method was established by enforced degradation studies combined with peak purity assessment using PDA detection. The method was linear over the concentration range of 5-150 μg mL −1 (r 2 = 0.9996) of ALI. Intraday and interday precision and accuracy evaluated by relative standard deviation, respectively, were lower than 2%. The limit of detection was 1.31 µg mL −1 . The method proved to be robust by a one-variable-at-a-time evaluation. The proposed MEKC method was successfully applied for the quantitative analysis of ALI in tablets to support the quality control.

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“…4 Recently, more methods were reported for the analysis of OFL including spectrophotometry 5 , HPLC [6][7][8][9][10][11][12] , HPTLC 10 and capillary electrophoresis. [13][14][15][16][17] FLV is also an official drug in the BP 3 that recommended a non-aqueous titrimetric method using perchloric acid as a titrant for its determination in pure form and a direct spectrophotometric method for its determination in tablets via measurement of its absorbance in 0.1 M HCl at 293 nm. A comprehensive review of the published analytical methods for determination of FLV up to 2001 was presented as a monograph in the series of "Analytical Profiles of Drug Substances and Excipients".…”

Section: Introductionmentioning

confidence: 99%

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

2015

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New sensitive and rapid micellar liquid chromatographic method was developed for determination of ofloxacin (OFL) and flavoxate hydrochloride (FLV) in different pharmaceutical formulations in addition to a pharmacokinetic study of OFL in human plasma and urine. The analyses were carried out on BDS Hypersil phenyl column (4.6 × 250 mm, 5 µm particle size) using a micellar mobile phase consisting of 0.15 M SDS, 15% n-propanol, 0.3% triethylamine and 0.02 M orthophosphoric acid (pH 2.5) with UV detection at 325 nm. The proposed method was found to be rectilinear over the concentration range of 2.0-40.0 µg mL -1 for the two drugs with lower detection limits of 0.16 and 0.32µg mL -1 for OFL and FLV, respectively. The proposed method was applied for determination of OFL and FLV in their single ingredient and co-formulated dosage forms with good percentage recoveries (99.86-99.98 %) and small standard deviation values (± 0.18 -1.26). The results of the proposed method were statistically compared with those obtained by the comparison methods revealing no significant differences in the performance of the two methods regarding accuracy and precision. To achieve high sensitivity that allows pharmacokinetic study of OFL, fluorescence detection at 290/485 nm was employed. A linear relationship was achieved over the concentration ranges of 0.01-0.10 and 0.02-0.20 µg mL -1 with mean percentage recoveries of 98.63 ± 9.84 and 100.63 ± 3.15 % for OFL in human plasma and urine, respectively.

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Simultaneous determination of ofloxacin and ornidazole in pharmaceutical preparations by capillary zone electrophoresis

Cited by 38 publications

References 22 publications

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

Stress degradation studies on aliskiren and the development of a sensitive stability-indicating MEKC method

Analysis of ofloxacin and flavoxate HCl either individually or in combination via a green chromatographic approach with a pharmacokinetic study of ofloxacin in biological samples

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