Simultaneous determination of Olanzapine and Fluoxetine in human plasma by LC–MS/MS: Its pharmacokinetic application

Bonde, Shantaram; Bhadane, Ranjana P.; Gaikwad, Avinash S; Gavali, S.R.; Katale, Deepak; Narendiran, A.S.

doi:10.1016/j.jpba.2013.10.033

Cited by 38 publications

(30 citation statements)

References 26 publications

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“…The mean values of the pharmacokinetic parameters were as follows: the maximum plasma concentration ( C max ), the time to reach the peak concentration ( T max ), and the area under the concentration–time curve (AUC 0– t and AUC 0–∞ ) (Table ). These findings were in agreement with published data . No statistically significant differences were found between the pharmacokinetic parameters of the test and reference formulation; the confidence interval of the C max was 83.15–99.23 and that of the AUC 0– t was 80.07–92.51.…”

Section: Resultssupporting

confidence: 92%

“…Of the 10 LLOQ methods described above, only three had an analytical run time of 2 min: Patel et al ., Bonde et al ., who used solid phase extraction and API‐4000 (MDS SCIEX, Toronto, ON, Canada), and the present study. Additionally, the current method showed a rapid analytical run time (2 min) with liquid–liquid extraction and Quattro LC.…”

Section: Discussionmentioning

confidence: 96%

“…These findings were in agreement with published data. 31,37 No statistically significant differences were found between the pharmacokinetic parameters of the test and reference formulation; the confidence interval of the C max was 83.15-99.23 and that of the AUC 0-t was 80.07-92.51.…”

Section: Application Of the Method: A Pharmacokinetic Studymentioning

confidence: 92%

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The development and validation of a method for quantifying olanzapine in human plasma by liquid chromatography tandem mass spectrometry and its application in a pharmacokinetic study

Bedor

Sousa

et al. 2015

Clin Exp Pharma Physio

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1. A rapid method using liquid chromatography tandem mass spectrometry for the quantification of olanzapine (OLZ) in human plasma was developed and validated. Venlafaxine was used as the internal standard (IS), and the samples were extracted from 400-μL human plasma with methyl tert-butyl ether for liquid-liquid extraction. 2. Chromatography was performed using an ACE C18, 125 × 4.6-mm i.d., 5-μm column. The mobile phase consisted of water with 0.1% formic acid for solvent A and acetonitrile with 0.1% formic acid for solvent B (50 : 50 v/v) in isocratic mode. The flow rate was 1.2 mL/min. The retention times for OLZ and the IS were 0.78 and 1.04 min, respectively. Tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used to detect OLZ and the IS (m/z: 313.1 > 256.1 and 278.1 > 260.2, respectively). 3. No significant matrix effects were observed on OLZ and the IS retention times, and the mean recovery of OLZ was 90.08%. The assay was linear in the concentration range of 1-20 ng/mL (R(2) = 0.9976). The intra- and inter-day precision were < 11.60% and the accuracy was < 1.66%. 4. This validated method was successfully applied to a pharmacokinetic study in which 10-mg OLZ tablets were administered to healthy volunteers and their plasma OLZ levels were monitored over time. The tests showed that the OLZ test and reference drug (Zyprexa(®)) were bioequivalent, as 90% of the confidence intervals were within the 80-125% interval proposed by regulatory agencies.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

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The development and validation of a method for quantifying olanzapine in human plasma by liquid chromatography tandem mass spectrometry and its application in a pharmacokinetic study

Bedor

Sousa

et al. 2015

Clin Exp Pharma Physio

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“…study the drug-drug interaction of methadone with antidepressive drugs. Though various methods were reported in the literature for estimation of methadone and fluoxetine/venlafaxine separately or in combination with other drugs [12][13][14][15][16], no method had been reported for simultaneous determination of these drugs using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). In the present study, we developed a UPLC-MS/MS method for the simultaneous determination of methadone, fluoxetine, venlafaxine and their main metabolites using diazepam as an internal standard in rat plasma, which was fully validated in terms of specificity, linearity, matrix effect and recovery, accuracy, precision and stability.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Methadone, Fluoxetine, Venlafaxine and Their Metabolites in Rat Plasma by UPLC–MS/MS for Drug Interaction Study

Pan

Wang

et al. 2016

Chromatographia

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“…[10][11][12][13][14][15][16][17][18] It is worth noting that no microextraction technique has been reported for simultaneous extraction and determination of OLZ and FLX from biological uids. [10][11][12][13][14][15][16][17][18] It is worth noting that no microextraction technique has been reported for simultaneous extraction and determination of OLZ and FLX from biological uids.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction and determination of trace amounts of olanzapine and fluoxetine from biological fluids: comparison of conventional hollow fiber supported liquid phase microextraction and pulsed electrically assisted liquid-phase microextraction techniques

2015

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In the current work, hollow fiber liquid phase microextraction (HF-LPME) and pulsed electromembrane extraction (PEME) coupled with high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection were compared for the extraction of olanzapine (OLZ) and fluoxetine (FLX) from human plasma and urine samples. The influences of extraction parameters affecting the extraction efficiency of OLZ and FLX were studied and optimized for both methods. Under passive extraction conditions, FLX and OLZ were extracted from alkaline samples (pH ¼ 12) into 1-octanol immobilized in the pores of a porous hollow fiber of polypropylene (SLM), and then transported into 25 mL of 25 mM HCl as the acceptor solution. Under electrokinetic migration conditions, FLX and OLZ were transported under applied voltage from acidic sample solutions (pH ¼ 6) through 2-nitrophenyl pentyl ether (NPPE) immobilized in the pores of the hollow fiber into 25 mL of 10 mM HCl as the acceptor solution. Under the optimized conditions, preconcentration factors in the range of 59 to 78 and 43 to 65 for PEME and HF-LPME, respectively, and satisfactory repeatabilities (3.4 < relative standard deviation [RSD] < 7.8) were obtained in different matrices. The obtained recoveries of the drugs in the range of 82.6-103.1%indicated the excellent capability of the developed methods for simultaneous extraction of FLX and OLZ from complex matrices. The results showed that in comparison with HF-LPME based on pH gradient, PEME is a more effective transport process, providing high extraction efficiencies in a short time.

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Simultaneous determination of Olanzapine and Fluoxetine in human plasma by LC–MS/MS: Its pharmacokinetic application

Cited by 38 publications

References 26 publications

The development and validation of a method for quantifying olanzapine in human plasma by liquid chromatography tandem mass spectrometry and its application in a pharmacokinetic study

The development and validation of a method for quantifying olanzapine in human plasma by liquid chromatography tandem mass spectrometry and its application in a pharmacokinetic study

Simultaneous Determination of Methadone, Fluoxetine, Venlafaxine and Their Metabolites in Rat Plasma by UPLC–MS/MS for Drug Interaction Study

Simultaneous extraction and determination of trace amounts of olanzapine and fluoxetine from biological fluids: comparison of conventional hollow fiber supported liquid phase microextraction and pulsed electrically assisted liquid-phase microextraction techniques

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