Simultaneous determination of pantothenic acid and hopantenic acid in biological samples and natural products by gas chromatography-mass fragmentography

Determination of Vitamin B5 in a range of fortified food products by reversed-phase liquid chromatography–mass spectrometry with electrospray ionisation

“…For Vitamin B 5 determination, deuterated compounds are not available. Thus, HOPA a non-labelled compound is the employed internal standard (IS) as it closely matches chemical and physical properties of Vitamin B 5 [13].…”

“…Although the sensitivity is adequate to determine low level of Vitamin B 5 in the product tested, the lack of selectivity due to the maximum of Vitamin B 5 absorption at about 200 nm where several compounds can interfere with the detection hampers the extension of the method to a wider range of products. Gas chromatography (GC) with flame ionisation detection has been used for the determination of Vitamin B 5 in food products [12], also Banno et al [13] applied GC-MS for the analysis of Vitamin B 5 and one of its metabolites HOPA (Fig. 1B) in biological fluid.…”

Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization

“…Radioisotopes and scintillation counting are required for the former, and non-commercially available antisera are needed for the latter. Methods using gas chromatography-mass spectrometry with multiple ion detection [11]; liquid chromatography-mass spectrometry * Corresponding author. Fax: +81 749 28 8499.…”

Determination of vitamin B5 in human urine by high‐performance liquid chromatography coupled with mass spectrometry

“…The detection of vitamin B 5 by UV in a complex matrix such as biological fluids remains difficult, as vitamin B 5 only absorbs at low wavelength (k a 220 nm) where several compounds in the sample to be analysed could co-elute or interfere with the vitamin B 5 peak. An alternative approach such as GC/MS was investigated [6] but the technique requires a derivatization step because vitamin B 5 lacks the volatility required for direct GC. In the literature no data are found concerning the metabolism and the excretion of vitamin B 5 in biological fluids such as urine.…”