Simultaneous determination of phenytoin, carbamazepine, and 10,11-carbamazepine epoxide in human plasma by high-performance liquid chromatography with ultraviolet detection

Liquid chromatography–electrospray mass spectrometry determination of carbamazepine, oxcarbazepine and eight of their metabolites in human plasma

“…In other published methods, acetonitrile was added to plasma samples to precipitate the plasma proteins [31,33,34]. In our study, protein precipitation was obtained by modification of the dielectric constant by using either methanol, acetonitrile and acetone.…”

“…To date, some HPLC methods with ultra-violet [17][18][19][24][25][26][27][28][29][30][31][32][33][34] or mass spectrometry [10,11,35,36] detection for determining CBZ and/or OXCBZ in biological fluids (plasma, urine) have been published. Some of them involved both the quantitation of CBZ or OXCBZ and their main metabolites (CBZ-EP, 10-OH-CBZ, DiOH-CBZ [19,24,[26][27][28][29][30][31]33,34]. Other methods allow to quantify either CBZ, CBZ-EP, 10-OH-CBZ, DiOH-CBZ and the 9-acridan [25,32], or the hydroxylated compounds (2-hydroxycarbamazepine (2-OH-CBZ); 3-OH-CBZ) [18].…”

Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring

“…The primary methods for the analysis of serum CBZ are commercial reagent-based techniques like as FPIA and EMIT [22,23]. However, there are also many GC and HPLC techniques with UV or mass spectrometry (MS) detection available and these have the advantage of simultaneously measuring other AEDs and the active metabolite CBZ-10,11-epoxide [15,16,[24][25][26][27][28]. But most of these assays employ UV detection, thereby increasing the risk of metabolite or matrix interferences.…”

“…However, there are also many GC with various detection [25,89] and HPLC methods have been reported [10,11,80,90]. Treatments for refractory epilepsy with a second drug or a combination of drugs indicate the need for simultaneous HPLC analysis for other AEDs [15,16,24,76,91,92].…”

Influence de la carbamazépine-10,11-époxyde dans le dosage de la carbamazépine « Dimension Flex® Carbamazepine »

“…Assay ont été utilisées sur les automates Dimension RxL et Cobas Mira respectivement. Les taux thérapeu-tiques de la carbamazépinémie ont été définis entre 4 et 12 mg/l par le fabriquant Dade Berhing.Les dosages de la carbamazépine et du dérivé époxyde par la méthode HPLC ont été réalisés suivant le protocole de dosage de Bhatti[2].…”