Simultaneous determination of quercetin, rutin and kaempferol in the leaf extracts of Moringa oleifera Lam. and Raphinus sativus Linn. by liquid chromatography-tandem mass spectrometry

Devaraj, V. C.; Krishna, Burdipad G; Viswanatha, Gollapalle Lakshminarayanashastry

doi:10.3736/jcim20110914

Cited by 30 publications

(16 citation statements)

References 21 publications

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“…The MS/MS fragment at m/z 303 matched with quercetin derivatives. In previous study, the presence of quercetin-3-rutinoside (rutin, compound 2) was revealed in asparagus (Devaraj et al 2011;Fuentes-Alventosa et al 2009a, b). Moreover, by comparison with MS, MS/MS data and UV spectrum of the standard compound, compound 2 was identified as quercetin-3-rutinoside (Del Rio et al 2004;Wang et al 2003).…”

Section: Hplc-esi-ms/ms Analysismentioning

confidence: 92%

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

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Asparagus residues were used as materials to obtain antioxidant compounds by solid-liquid extraction in this study. The effects of different extraction parameters including extraction solvents, time, temperature and liquid-solid ratio on the contents of total flavonoids, total phenolics and total antioxidant activity were investigated. Antioxidant activity of the extract from asparagus residues was evaluated by HPLC-ABTS· + and the bioactive components were identified by HPLC-MS/MS. The results showed that the extraction yield was significantly influenced (P<0.05) by solvent composition, extraction time and temperature. The appropriate parameters were preferred as extraction solvent of 50 % ethanol with liquid-solid ratio of 30:1, extraction temperature of 80°C and time of 2 h. Antioxidant activity evaluation of the extract indicated flavonoids and phenolics were dominant bioactive compounds. Five antioxidant compounds were identified as ferulic acid, kaempferol, quercetin, rutin and isorhamnetin.

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Section: Hplc-esi-ms/ms Analysismentioning

confidence: 92%

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

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“…and Raphinus sativus Linn. [10], in Apocynum venetum [11], and in another for Gingko biloba solid oral dosage forms [12]. These methods cannot be applied directly for the estimation in polyherbal formulation and plant extracts because of complexity in the nature and intrinsic variability of chemical constituents.…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS/MS

Satheeshkumar

Saladi

Komali

2014

Chromatography Research International

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Adulteration of herbal supplements is a major issue for many countries. A simple and reliable HPLC-PDA method was developed for quantification of aldose reductase inhibitory flavonoids rutin, quercetin, and kaempferol. The chromatographic separation was performed on a Fortis C 18 column in gradient mode with detection at 267 nm. The presence of these markers was confirmed through the accurate m/z values and MS/MS data obtained using quadruple time of flight mass spectrometry (QTOF-MS). The proposed method was successfully applied to examine the amount of these active constituents in antiobese polyherbal formulation and plant extract of Gymnema sylvestre.

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“…The 2 characteristic product ions in the ESI‐MS/MS spectrum of protonated rutin are the quercetin moiety at m/z 303 and the ion formed by the cleavage of the glycosidic bond at the non‐reducing side of the glycosidic oxygen (referred to as a Y‐type ion) at m/z 465 . As seen in Figure , a product ion at m/z 303 can also be observed in the MS/MS spectrum of rutin derivatized with 2 and 3 TMS groups, which occur at m/z 447 and 519, respectively.…”

Section: Resultsmentioning

confidence: 97%

“…The 2 characteristic product ions in the ESI-MS/MS spectrum of protonated rutin are the quercetin moiety at m/z 303 and the ion formed by the cleavage of the glycosidic bond at the non-reducing side of the glycosidic oxygen (referred to as a Y-type ion 18 ) at m/z 465. 4,19 As seen in Figure 3…”

Section: Tandem Mass Spectrometrymentioning

confidence: 90%

“…Samples are analyzed by thermal desorption from a surface in the DART ion source; therefore, the efficiency of DART ionization strongly depends on the volatility and thermal stability of the analyte. 7 For instance, rutin (quercetin-3-O-rutinoside) cannot be detected by DART-MS, and silybin ((2R,3R)-3,5,7-trihydroxy-2-[3-(4-hydroxy-3methoxyphenyl)-2-hydroxymethyl-2,3-dihydrobenzo [1,4]dioxin- 6-yl] chroman-4-one), the major component of silymarin, is difficult to detect by this method due to the low volatility and thermal instability of these compounds.…”

Section: Introductionmentioning

confidence: 99%

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Rapid qualitative analysis of 2 flavonoids, rutin and silybin, in medical pills by direct analysis in real‐time mass spectrometry (DART‐MS) combined with in situ derivatization

et al. 2018

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Direct analysis in real-time mass spectrometry (DART-MS) with in situ silylation was used for the rapid analysis of the flavonoids silybin ((2R,3R)-3,5,7-trihydroxy-2-[3-(4-hydroxy-3-methoxyphenyl)-2-hydroxymethyl-2,3-dihydrobenzo[1,4]dioxin-6-yl]chroman-4-one) and rutin (quercetin-3-O-rutinoside). Three different derivatization reagents, hexamethyldisilazane/trimethylchlorosilane/pyridine (HMDS/TMCS/pyridine), N,O-bis(trimethylsilyl)acetamide/trimethylchlorosilane/N-trimethylsilyimidazole (BSA/TMCS/TMSI), and N,O-bis(trimethylsilyl)trifluoroacetamide/trimethylchlorosilane (BSTFA/TMCS), were applied. Silybin and rutin were detected with various degrees of silylation, and the formation of dimers with pyridine and imidazole was also observed. HMDS/TMCS/pyridine was the best choice for the DART-MS analysis of silybin, and BSA/TMCS/TMSI was the most effective for the detection of rutin. The effects of the DART source temperature on desorption, ionization, in-source fragmentation, dimer formation, and hydrolysis of the trimethylsilyl groups were also studied. In addition, the collision-induced dissociation properties of the derivatized silybin and rutin were explored. With our in situ silylation method, the derivatized bioactive compounds in intact medical pills could also be detected by DART-MS.

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Simultaneous determination of quercetin, rutin and kaempferol in the leaf extracts of Moringa oleifera Lam. and Raphinus sativus Linn. by liquid chromatography-tandem mass spectrometry

Cited by 30 publications

References 21 publications

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS/MS

Rapid qualitative analysis of 2 flavonoids, rutin and silybin, in medical pills by direct analysis in real‐time mass spectrometry (DART‐MS) combined with in situ derivatization

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