Simultaneous determination of salbutamol sulphate and bromhexine hydrochloride in tablets by reverse phase liquid chromatography

Pai, P. N. S.; Rao, Gopal Krishna; Murthy, M. Srinivasa; Agarwal, Ankit; Puranik, S. B.

doi:10.4103/0250-474x.51957

Cited by 22 publications

(11 citation statements)

References 1 publication

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“…Several analytical methods for the determination of salbutamol were developed and reported such as TLC (Dave et al, 2011), GC and GC-MS (Caban et al, 2011;Wang et al, 2010), cyclic voltammetry (Ganjali et al, 2005, adsorptive stripping voltammetry on a carbon paste electrode (Attaran et al, 2012), Capillary electrophoresis coupled with electrochemilumencesis (Bao et al, 2012), flow injection method (Al-Abachi and Subhi, 2013), HPLC and RP-HPLC (Mukesh and Ranjit, 2011;Pai et al, 2009 ;Ghulam et al, 2009 ;Li et al, 2010), HPLC-chemiluminescence (Zhang et al, 2011), solid phase extraction (SPE)-HPLC (Liu and Wang , 2011;Yan et al, 2012) , and fluorescence (Tang et al, 2010).…”

Section: Salbutamol Sulphatementioning

confidence: 99%

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

Othman¹,

Asaad²

2014

RJS

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A simple, rapid and sensitive spectrophotometic method for the estimation of trace amounts of salbutamol sulphate (SBS) in pharmaceutical preparations has been proposed .The method is based on the coupling reaction of the intended compound with diazotized 5-amino-2chlorobenzotrifluoride in alkaline medium and in the presence of Triton X-100 to form a yelloworange dye that shows maximum absorption at 467 nm. Beer's law is obeyed over the range 5-300 µg / 10 ml (i.e.,0.5-30 ppm) with a molar absorptivity of 4.20 × 10 4 l.mol-1 .cm-1 and Sandell's sensitivity index of 0.0139 µg.cm-2 , a relative error of +1.08 to +3.46 % and a relative standard deviation of ±0.76 to ±3.50 %, depending on the concentration. The method has been applied to estimate salbutamol sulphate in syrup, tablet and ventolin.

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Section: Salbutamol Sulphatementioning

confidence: 99%

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

Othman¹,

Asaad²

2014

RJS

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“…[4] Several methods have been reported spectrophotometry, [5][6][7] high-performance liquid chromatography (HPLC). [8][9][10][11][12][13][14] Cephalexin [ Figure 1] is chemically {(6R, 7R)-7-[(R)-2-amino-2-phenylacetamido]-3-methyl-8-oxo-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid monohydrate} [15] which is a first-generation cephalosporins for oral administration which is bactericidal and mainly used in the treatment of various bacterial infections caused by Grampositive and Gram-negative microorganisms. [16] Cephalexin is official in IP, [17] United States Pharmacopoeia, [18] and BP.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Kumar¹

2017

AJP

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Aim: The aim of this study is to develop two simple, rapid, accurate, economical validated spectrophotometric for simultaneous estimation of bromhexine and cephalexin in bulk and dosage form. Materials and Methods: The Method-I is first derivative spectrophotometric and Method-II is the simultaneous equation method. Both the methods were employed for the analysis of drugs bromhexine and cephalexin using methanol and ammonium hydrogen phosphate (pH 7.7) in the ratio of 60:40 as a diluent. Results and Discussions: Both the methods are used for the simultaneous determination of bromhexine and cephalexin at 2155 nm and 266.5 nm, respectively, over the concentration range of 0.2-1.2 μg/ml and 12.5-75 μg/ml with the correlation coefficient (r 2 ) 0.9996 and 0.9994. Conclusions: The recovery studies confirmed the accuracy of proposed method and low-value standard deviation confirmed precession of method. The method is validated as per the International Council for Harmonization guidelines.

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“…Some of the reported methods for racemic salbutamol sulfate includes titrimetric (Basavaiah et al, 2006(Basavaiah et al, , 2007, potentiometric (Abdel-Ghani et al, 2002), spectrophotometric (Basavaiah et al, 2003(Basavaiah et al, , 2006(Basavaiah et al, , 2007Chitlange et al, 2011;Culzoni et al, 2006; Geeta et al ., 1989;Habib et al, 2004;Jain et al, 2011;Mishra et al, 2010;Parimoo et al, 1993;Rathore et al, 1993), spectrofluorimetric (Pandya et al, 2010), HPLC (Bernal et al, 1996;Boulton et al, 1995;Chitlange et al, 2011;Erram et al, 2006;Jain et al, 2011;Kasawar et al, 2010;Kountourellis et al, 1990;Martis et al, 2011;Murtaza et al, 2009;Pai et al, 2009) and LC-MS (Joyce et al, 1998;Wu et al, 2004Wu et al, , 2011Yee et al, 2011). Whereas for levosalbutamol sulfate a few spectrophotometric Thulasama et al, 2009) and HPLC (Ghemud et al, 2012;Halabi et al, 2004; methods are reported.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

Panda

Kumar

Mohanta

2013

Braz. J. Pharm. Sci.

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A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C 18 column (250 mm × 4.6 mm i.d., 5 μm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min -1 . The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 μg.mL -1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, oxidation, thermal and photolytic degradation. The method was used successfully for the simultaneous determination of levosalbutamol sulfate and theophylline in syrup dosage form.Uniterms: Levosalbutamol/determination. Theophylline/determination. Stability-indicating. RP-High performance liquid chromatography/qualitative analysis Syrup/qualitative analysis.Desenvolveu-se e validou-se método de RP-HPLC novo, simples, exato e preciso de determinação simultânea do sulfato de levossalbutamol e teofilina.. A separação foi efetuada em uma coluna Phenomenex; C18 (250 mm x 4,6 mm d.i., 5 μm) utilizando metanol: TBAHS (hidrogenossulfato de tetrabutilamônio) 10 mM (50:50, v/v) como fase móvel, com fluxo de 1,0 mL.min -1 . O comprimento de onda de detecção no UV foi 274 nm. Observou-se linearidade na faixa de concentração de 0,5-150 μg mL -1 , com coeficiente de correlação de 0,999 para ambos os fármacos. O método proposto foi validado determinando-se exatidão, precisão, estabilidade e parâmetros de adequação do sistema. O método mostrou-se robusto. A especificidade do método foi determinada submetendo os fármacos a várias condições de estresse, como ácido, álcali, oxidação, degradação térmica e fotolítica. O método foi usado com sucesso para a determinação simultânea do sulfato de levossalbutamol e teofilina na forma de xarope.

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Simultaneous determination of salbutamol sulphate and bromhexine hydrochloride in tablets by reverse phase liquid chromatography

Cited by 22 publications

References 1 publication

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

Spectrophotometric Determination of Salbutamol Sulphate by Coupling with Diazotized 5-Amino-2-chlorobenzotrifluoride – Application to Pharmaceutical Preparations

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Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

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