Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

Panda, Sagar Suman; Kumar, B. V. V. Ravi; Mohanta, Ganeswar

doi:10.1590/s1984-82502013000300009

Cited by 9 publications

(4 citation statements)

References 44 publications

(34 reference statements)

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“…The representative concentrations of ceftiofur (0.1 to 10 µg/mL) were plotted against corresponding peak areas and resultant calibration curve was used to evaluate the linearity of HPLC method. 13 The correlation co-efficient, slope and intercept of standard curve were calculated.…”

Section: Linearitymentioning

confidence: 99%

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

Adil

Omer

Javeed

et al. 2019

ACSi

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We optimized and validated an isocratic high-performance liquid chromatography (HPLC) assay for quantification of ceftiofur hydrochloride in bubaline plasma. Ceftiofur, its metabolic products and protein-bound residues were cleaved, derivatized into desfuroylceftiofur acetamide and injected into HPLC system. The mobile phase comprising of sodium dihydrogen phosphate (0.025 M, pH 7) and acetonitrile (34:66, v/v), was driven at a flow rate of 1 mL/min, and separation was achieved using C18 column. Isocratic elution was performed with an injection volume of 45 µL and analyte was scanned at 310 nm. The linearity range, limit of detection and limit of quantification were 0.1-10 µg/mL, 0.03 µg/mL and 0.11 µg/mL respectively. Moreover, the accuracy, precision and recovery remained within the acceptable limits. The assay was effectively applied for determining the concentration of ceftiofur in plasma samples collected from ceftiofur-treated buffalo calves.

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Section: Linearitymentioning

confidence: 99%

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

Adil

Omer

Javeed

et al. 2019

ACSi

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“…Ipratropium bromide is a bronchodilator that works via blockade of muscarinic cholinergic receptors and is clinically used in combination with other inhalation solutions or suspensions for enhanced treatment efficacy [3]. It is used for various bronchial disorders, in rhinitis, and as an antiarrhythmic [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of stability-indicating RP-HPLC method for the simultaneous estimation of xylometazoline hydrochloride and ipratropium bromide from nasal spray dosage form

Musmade¹,

Sanatan²,

Lokhande³

et al. 2021

Futur J Pharm Sci

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Background A simple, robust, precise, and an accurate HPLC method was established for simultaneous estimation of xylometazoline hydrochloride and ipratropium bromide from a nasal spray dosage form. The effective separation was obtained by injecting 10 μl of sample and standard solutions on to an Inertsil ODS column, 250 × 4.6, mm, 5 μ at 45 °C using phosphate buffer with 1-pentane sulphonic acid sodium salt at pH 4.7 as a mobile phase A and acetonitrile as the mobile phase B. The gradient was optimized with a flow rate of 1 mL/min and a wavelength of 210.0 nm. Result The complete analytical method validation was successfully carried out as per ICH guidelines. The retrieval study was carried out at 50% to 150% level of working concentration, and results were in the range of 99 to 101% for both the analytes. The linearity was proven from 4 to 150% of working concentration with linear regression curve (R2=0.999) for both the analytes. The developed method was robust for different parameters like column temperature, flow rate, mobile phase pH, composition, and gradient. Conclusion The developed HPLC method can be successfully used for the estimation of xylometazoline hydrochloride and ipratropium bromide from nasal spray dosage form as a release test in QC department of manufacturing units.

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“…This combination is used for a treatment of bronchial asthma and asthmatic bronchitis conditions [1,2]. The reversed phase high performance liquid chromatographic (HPLC) method has been widely used for the assay of one or two active ingredients simultaneously in dosages form containing TH, GF and or DH, or other active ingredients [3][4][5][6][7][8]. The HPLC methods have also been used for the simultaneous analysis of these three active ingredients in a mixture with some other active substances or excipients using acetonitrile-phosphate buffer pH 3.2 as mobile phase [9,10].…”

Section: Introductionmentioning

confidence: 99%

Development of a high performance liquid chromatography method for simultaneous analysis of theophylline, guaifenesin and diphenhydramine in an elixir

Hayun¹,

Puspasari²

2017

Trop. J. Pharm Res

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Purpose: To develop and validate a new low-cost high performance liquid chromatography (HPLC) method for simultaneous analysis of theophylline (TH), guaifenesin (GF) and diphenhydramine hydrochloride (DH) in elixir dosage form.Methods: Chromatographic conditions were an isocratic elution with C18-Kromasil® column (250 x 4.6 mm, 5 μm), methanol-water (1:1, v/v, pH 3,0) as mobile phase, flow rate 1.0 ml/min and UV detector at λ 218 nm. The method was validated for selectivity, linearity, LOD-LOQ, precision, and accuracy.Results: Retention time of TH, GF and DH was 3.3, 5.3 and 9.1 min, respectively. The method showed good selectivity, calibration curves were linear over the concentration range of 1.000 – 10.002 μg/mL, 0.801 – 8.008 μg/mL, and 0.251 – 2.514 μg/mL (r2 > 0.999). LOD was 0.1093, 0.16520, and 0.0706 μg/mL, while LOQ was 0.3645, 0.5506, and 0.2354 μg/mL for TH, GF and DH, respectively. Recovery accuracy was 99.77 - 101.10, 100.50 - 101.95 and 99.20 - 100.13 % for TH, GF and DH, respectively; precision (RSD) was < 2.0.Conclusion: The proposed method is highly selective, sensitive, precise, and accurate, and would suitable for the simultaneous analysis of TH, GF, and DH in elixir dosage form. Since methanol is cheaper than acetonitrile, the application of the method may reduce the cost of analysis.Keywords: Simultaneous analysis, Theophylline, Guaifenesin, Diphenhydramine hydrochloride, Elixir

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Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

Cited by 9 publications

References 44 publications

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

Isocratic High Performance Liquid Chromatography Assay for Quantification of Ceftiofur Hydrochloride in Bubaline Plasma

Development and validation of stability-indicating RP-HPLC method for the simultaneous estimation of xylometazoline hydrochloride and ipratropium bromide from nasal spray dosage form

Development of a high performance liquid chromatography method for simultaneous analysis of theophylline, guaifenesin and diphenhydramine in an elixir

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