Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography

Mateus, Lidia; Cherkaoui, Samir; Christen, Philippe; Oksman-Caldentey, Kirsi-Marja

doi:10.1016/s0031-9422(00)00134-5

Cited by 51 publications

(20 citation statements)

References 30 publications

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“…The extraction procedure of Flos daturae used here was a modification of the technique reported by Kursinszki [15] and Mateus et al [22]. About 0.5 g powdered herbal medicine was weighted accurately and transferred to 20 mL tube.…”

Section: Sample Preparationmentioning

confidence: 99%

“…A validated capillary zone electrophoresis method coupled with ultraviolet detection has been described for the determinations of atropine and scopolamine in ophthalmic solutions [18] and the main tropane alkaloids in Hyoscyamus muticus [24 -26], Datura metel [27] or Flos daturae [28] plants. Hyoscyamine and scopolamine in plant extracts can also be detected using micellar electrokinetic chromatographic method with ultraviolet detection [22,23]. This work proposed a novel method for simultaneous determination of atropine, scopolamine and anisodamine in Flos daturae plants with CE coupled with electrochemiluminescence (ECL) detection using b-cyclodextrin as a buffer additive to improve their separation.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Electrochemiluminescence Detection of Anisodamine, Atropine, and Scopolamine in Flos daturae by Capillary Electrophoresis Using β‐Cyclodextrin as Additive

Chun

2007

Electroanalysis

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This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturae based on capillary electrophoresis coupled with electrochemiluminescence detection. b-Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 mM for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Electrochemiluminescence Detection of Anisodamine, Atropine, and Scopolamine in Flos daturae by Capillary Electrophoresis Using β‐Cyclodextrin as Additive

Chun

2007

Electroanalysis

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“…The hairy roots in Capsicum species was initiated through A. rhizogenes strains has also been reported (Setamam et al, 2014). From the research findings of (Vanhala et al, 1995;Mateus et al, 2000) where they reported that Agrobacterium infection was shown to increase root growth and higher accumulation of alkaloids and tropane content in Hyoscyamus muticus transgenic root cultures. Gentiana macrophyllahairy root cultures were evaluated after infection with four A. rhizogenes strains,where different responses were observed in each hairy root linefor their root growth and also for accumulation of secoiridoid glucoside, gentiopicroside (Tiwari et al, 2007).…”

Section: Discussionmentioning

confidence: 93%

Response of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache Rugosa

Park

Baskar

Yeo

et al. 2017

OnLine Journal of Biological Sciences

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In this study a total of seven different Agrobacterium rhizogenes strains were evaluated for their ability to transform the plant Agastache rugosa and to produce the secondary metabolite rosmarinic acid. All the strains of A. rhizogenes i.e., 13333, 15834, A 4, LBA9402, R1000, R1200 and R1601 strains tested here in this study, were able to induce hairy root formation in leaf tissue explants. The strain A 4 had the highest rate of infection (94.1±8.3%) and the strain R1000 had the lowest rate (88.6±6.9%). The highest frequency of hairy roots per explant (13.6±1.4) was found for strain R1601 and the tallest root length (20.4±1.7 mm) was found for strain 13333. We also evaluated dry weight and level of rosmarinic acid in the hairy roots and found that the highest growth (310.1±14.6 mg/flask) was occurred after infection with strain R1200, while the highest production of rosmarinic acid (68.2±3.8 mg/g dry weight) was noted using strain 13333. Our study showed that A. rhizogenes strain 13333 was the most effective of the 7 tested strains for production of transformed root cultures as well as rosmarinic acid in the hairy roots.

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“…Hyoscyamus a small herbaceous genus having 26 species all over the world (Yousaf et al 2008) and is represented by 6 species in Turkey (Baytop 1978;Güner et al 2000;Güner 2012). Hyoscyamus species have medicinal importance because of their hyoscyamine and scopolamine content (Mateus et al 1998(Mateus et al , 2000. Therefore, it is widely used as sedative and painless in folk medicine (Baytop 1999).…”

Section: Introductionmentioning

confidence: 99%

Comparative anatomical studies on some species of Hyoscyamus L. (Solanaceae) growing in Turkey

Satil

Aslant

et al. 2015

Bangladesh J. Bot.

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A comparative study based on leaf and stem anatomical structure was made using light microscopy (LM) techniques on five species of Hyoscyamus L. (Solanaceae) in Turkey. Some characters are found important to distinguish the species within the genera. The investigated species can be divided as mesophyll type: bifacial (H. niger L., H. albus L. ) and equifacial (H. aureus L., H. pusillus L., H. reticulatus L.). Druse crystals are recorded only in mesophyll of H. albus. Stomata present on both surfaces, are anisocytic (usually) and anomocytic types. H. reticulatus can be distinguished from other species considering types of trichomes in the stem. Vascular bundles are bicollateral types.

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Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography

Cited by 51 publications

References 30 publications

Simultaneous Electrochemiluminescence Detection of Anisodamine, Atropine, and Scopolamine in Flos daturae by Capillary Electrophoresis Using β‐Cyclodextrin as Additive

Simultaneous Electrochemiluminescence Detection of Anisodamine, Atropine, and Scopolamine in Flos daturae by Capillary Electrophoresis Using β‐Cyclodextrin as Additive

Response of Different <i>Agrobacterium Rhizogenes</i> Strains for <i>in vitro</i> Hairy Root Induction and Accumulation of Rosmarinic Acid Production in <i>Agastache Rugosa</i>

Comparative anatomical studies on some species of Hyoscyamus L. (Solanaceae) growing in Turkey

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