Simultaneous determination of seven flavonoids in Epimedium by liquid chromatography–tandem mass spectrometry method

Wu, Cai Sheng; Guo, Baoyuan; Sheng, Yu Xin; Zhang, Jin Lan

doi:10.1016/j.cclet.2008.01.018

Cited by 16 publications

(11 citation statements)

References 8 publications

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“…There are many different Epimedium species reported [3,4]. E. brevicornu, E. koreanum, E. pubescens, E. sagittatum, and E. wushanese are the five major species [7].…”

Section: Application To Measure the Two Breviflavones In Ethanolic Exmentioning

confidence: 99%

“…Flavonoids have been proven to be the bioactive components in Epimedium herbs, and more than 60 flavonoids have been isolated from Epimedium herbal extracts [4,5]. Epimedium herbs contain major components, e.g., epimedium A, B, C, and icariin, etc., and many minor components such as apigenin, luteolin, kaempferol, quercetin and so on [3,4]. Two new types of minor flavonoids, breviflavone A and B, have been recently isolated and identified from Epimedium brevicornu in our previous research [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…However, these methods suffered from low resolution or low sensitivity for minor flavonoids. Recently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been applied for qualitative and quantitative analyses of flavonoids in Epimedium herbal extracts [4,13] and in human [14] and rat plasma samples [15] after administration of Epimedium herbal products. Those reported LC-MS/MS methods provided high specificity, high resolution and sensitivity for determination of both major and minor flavonoids in Epimedium herbal samples.…”

Section: Introductionmentioning

confidence: 99%

“…For the quality control of botanicals and herbal extracts, the consistency and stability of bioactive constituents of different batches of herbs are critical [3,6]. It is reported that Epimedium herbs have five major species (E. brevicornu, E. koreanum, E. pubescens, E. sagittatum, and E. wushanese) [7] and at least other thirteen minor species [3,4]. Our previous research showed that the breviflavone B is the most potent estrogenic component in those Epimedium species [2,3].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Determination of breviflavone A and B in Epimedium herbs with liquid chromatography–tandem mass spectrometry

Hong

Wang

Yong

et al. 2009

Journal of Pharmaceutical and Biomedical Analysis

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“…There are many different Epimedium species reported [3,4]. E. brevicornu, E. koreanum, E. pubescens, E. sagittatum, and E. wushanese are the five major species [7].…”

Section: Application To Measure the Two Breviflavones In Ethanolic Exmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of breviflavone A and B in Epimedium herbs with liquid chromatography–tandem mass spectrometry

Hong

Wang

Yong

et al. 2009

Journal of Pharmaceutical and Biomedical Analysis

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“…LC-MS was also used for the quantification of flavonoids(Islam et al, 2008;Wu et al, 2008b;Hong et al, 2009), especially in pharmacokinetic studies…”

mentioning

confidence: 99%

Chemical Constituents, Quality Control, and Bioactivity of Epimedii Folium (Yinyanghuo)

Chen

Tang

et al. 2015

Am. J. Chin. Med.

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Epimedii Folium (Yinyanghuo in Chinese) is one of the most commonly used traditional Chinese medicines. Its main active components are flavonoids, which exhibit multiple biological activities, such as promotion of bone formation and sexual function, protection of the nervous system, and prevention of cardiovascular diseases. Flavonoids also show anti-inflammatory and anticancer effects. Various effective methods, including genetic and chemical approaches, have been developed for the quality control of Yinyanghuo. In this review, the studies conducted in the last decade about the chemical constituents, quality control, and bioactivity of Yinyanghuo are summarized and discussed.

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Geographical classification of Epimedium based on HPLC fingerprint analysis combined with multi‐ingredients quantitative analysis

Zhou

et al. 2016

Biomedical Chromatography

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A reliable and comprehensive method for identifying the origin and assessing the quality of Epimedium has been developed. The method is based on analysis of HPLC fingerprints, combined with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and multi-ingredient quantitative analysis. Nineteen batches of Epimedium, collected from different areas in the western regions of China, were used to establish the fingerprints and 18 peaks were selected for the analysis. Similarity analysis, HCA and PCA all classified the 19 areas into three groups. Simultaneous quantification of the five major bioactive ingredients in the Epimedium samples was also carried out to confirm the consistency of the quality tests. These methods were successfully used to identify the geographical origin of the Epimedium samples and to evaluate their quality.

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Simultaneous determination of seven flavonoids in Epimedium by liquid chromatography–tandem mass spectrometry method

Cited by 16 publications

References 8 publications

Determination of breviflavone A and B in Epimedium herbs with liquid chromatography–tandem mass spectrometry

Determination of breviflavone A and B in Epimedium herbs with liquid chromatography–tandem mass spectrometry

Chemical Constituents, Quality Control, and Bioactivity of Epimedii Folium (Yinyanghuo)

Geographical classification of Epimedium based on HPLC fingerprint analysis combined with multi‐ingredients quantitative analysis

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