“…However, it is recommended to use the matrix surrogate calibration curve for the analysis of metabolites in biological samples. 86 Compared to many available studies, LLOQ values for T, 34,39,47,81,82,85 DHEA, 35,39,41,45,47,81,82,85 PROG, 35,39,41,44,47 PREG, 39,47 ANDRO, 39,41,81,82,85 ALLO, 88,89 and DHT 39 were better or at least within 1 order of magnitude. Lower quantification limits for some substances in other methods may be due to the use of different ionization techniques.…”