Simultaneous Determination of Seven Plant Growth Regulators in Melons and Fruits by Modified QuEChERS Coupled with Capillary Electrophoresis

Chen, Yaling; Wu, Xin; Li, Yongxin; Yang, Yi; Yang, Dan-ni; Yin, Shuo; Lin, Lianzhu; Sun, Chengjun

doi:10.1007/s12161-018-1266-2

Cited by 13 publications

(3 citation statements)

References 32 publications

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“…Gibberellic acid GA3 is the most common gibberellin (Kwon & Paek, 2016), and it participates during seed germination, stem elongation and the beginning of flowering (Camara et al, 2018). To our knowledge, several studies focus on the individual and simultaneous determination of phytohormones in different matrices have been established using high performance liquid chromatography (HPLC) (Nakurte et al, 2012;Nhujak et al, 2005;Rivera et al, 2020;Spagnuolo et al, 2022;Wu & Hu, 2009), liquid chromatography with tandem mass spectrometry (LC-MS-MS) (Yang et al, 2022), capillary electrophoresis coupled to mass spectrometry (CE-MS) (Tan et al, 2014), as well as capillary electrophoresis coupled to a diode array detector (CE-DAD) (Chen et al, 2018;Huang et al, 2014;Nhujak et al, 2005;Quijano-Avilés et al, 2018;Yang et al, 2016). Furthermore, most of the procedures require sample preparation steps such as solid-liquid extraction, solid-phase extraction (SPE), supercritical fluid extraction (SFE), liquid-phase microextraction (LPME), ultrasound-assisted extraction (UAE), dynamic sonication-assisted solvent extraction (DSASE), among other techniques.…”

Section: Introductionmentioning

confidence: 99%

Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection

Chóez-Guaranda,

Rendon,

Peralta

et al. 2024

Sci. agropecu.

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Phytohormones, also known as plant hormones, are naturally occurring chemical compounds that regulate various physiological processes in plants. In this work, a capillary electrophoretic coupled to a diode array detector (CE-DAD) method was developed and validated for the simultaneous quantification of abscisic acid (ABA), 3-indolacetic acid (IAA), and gibberellic acid (GA3) in commercial plant biostimulants. Sample preparation was carried out by liquid-liquid extraction using ethyl acetate. CE separation was performed in a fused-silica capillary and background electrolyte (BGE) consisting of borate buffer (50 mM, pH 9.3) applying a high voltage of 20 kV, a pressure of 50 mbar, and injection time of 35 s. The ABA, IAA, and GA3 were detected at 254, 220 and 195 nm respectively. The CE-DAD method validation results showed acceptable specificity, linearity, accuracy, and precision in the concentration range of 10-100 µg/mL for all compounds according to the (International Conference Harmonisation) ICH guidelines. The proposed method was satisfactory applied to the analysis of cited plant hormones in biostimulants and suggest that sample preparation is a reliable step for extraction of phytohormones containing carboxyl groups. Therefore, the developed and validated method could be implemented as a low-cost and fast analytical tool for quality control purposes.

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Section: Introductionmentioning

confidence: 99%

Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection

Chóez-Guaranda,

Rendon,

Peralta

et al. 2024

Sci. agropecu.

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“…Plant physiological processes are usually the result of network regulation of a variety of phytohormones [ 2 ]; thus, it is necessary to develop an effective method for simultaneous detection of phytohormones. At present, the established methods for phytohormone detection mainly include chromatography [ 3 , 4 ], liquid chromatography tandem mass spectrometry [ 5 ], capillary electrophoresis [ 6 ], fluorescence method [ 7 ], and electrochemical method [ 8 , 9 , 10 ], among others. Among the many detection methods, the electrochemical method is favored because of its convenient operation, inexpensive equipment, rapid response, and high sensitivity [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Electrochemical Sensing of Indole-3-Acetic Acid and Salicylic Acid on Poly(L-Proline) Nanoparticles–Carbon Dots–Multiwalled Carbon Nanotubes Composite-Modified Electrode

Kuang

Fan

et al. 2022

Sensors

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Sensitive simultaneous electrochemical sensing of phytohormones indole-3-acetic acid and salicylic acid based on a novel poly(L-Proline) nanoparticles–carbon dots composite consisting of multiwalled carbon nanotubes was reported in this study. The poly(L-Proline) nanoparticles–carbon dots composite was facilely prepared by the hydrothermal method, and L-Proline was used as a monomer and carbon source for the preparation of poly(L-Proline) nanoparticles and carbon dots, respectively. Then, the poly(L-Proline) nanoparticles–carbon dots–multiwalled carbon nanotubes composite was prepared by ultrasonic mixing of poly(L-Proline) nanoparticles–carbon dots composite dispersion and multiwalled carbon nanotubes. Scanning electron microscope, transmission electron microscope, Fourier transform infrared spectroscopy, ultraviolet visible spectroscopy, energy dispersive spectroscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and linear sweep voltammetry were used to characterize the properties of the composite. poly(L-Proline) nanoparticles were found to significantly enhance the conductivity and sensing performance of the composite. Under optimal conditions, the composite-modified electrode exhibited a wide linear range from 0.05 to 25 μM for indole-3-acetic acid and from 0.2 to 60 μM for salicylic acid with detection limits of 0.007 μM and 0.1 μM (S/N = 3), respectively. In addition, the proposed sensor was also applied to simultaneously test indole-3-acetic acid and salicylic acid in real leaf samples with satisfactory recovery.

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“…Therefore, it is necessary to establish a method for the determination of IAA and SA with high selectivity and sensitivity. The current detection methods include liquid chromatography-mass spectrometry [5,6], molecular imprinting method [7,8], capillary electrophoresis [9], fluorescence spectroscopy [10] and electrochemical method [11]. Among them, electrochemical method is favored for its advantages of simple operation, high sensitivity, and fast analysis compared with other methods.…”

Section: Introductionmentioning

confidence: 99%

One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid

Kuang

et al. 2022

Sensors

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A sensitive simultaneous electroanalysis of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA) based on a novel copper nanoparticles-chitosan film-carbon nanoparticles-multiwalled carbon nanotubes (CuNPs-CSF-CNPs-MWCNTs) composite was reported. CNPs were prepared by hydrothermal reaction of chitosan. Then the CuNPs-CSF-CNPs-MWCNTs composite was facilely prepared by one-step co-electrodeposition of CuNPs and CNPs fixed chitosan residues on modified electrode. Scanning electron microscope (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV) were used to characterize the properties of the composite. Under optimal conditions, the composite modified electrode had a good linear relationship with IAA in the range of 0.01–50 μM, and a good linear relationship with SA in the range of 4–30 μM. The detection limits were 0.0086 μM and 0.7 μM (S/N = 3), respectively. In addition, the sensor could also be used for the simultaneous detection of IAA and SA in real leaf samples with satisfactory recovery.

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Simultaneous Determination of Seven Plant Growth Regulators in Melons and Fruits by Modified QuEChERS Coupled with Capillary Electrophoresis

Cited by 13 publications

References 32 publications

Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection

Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection

Simultaneous Electrochemical Sensing of Indole-3-Acetic Acid and Salicylic Acid on Poly(L-Proline) Nanoparticles–Carbon Dots–Multiwalled Carbon Nanotubes Composite-Modified Electrode

One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid

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