Simultaneous determination of simvastatin and its hydroxy acid form in human plasma by high-performance liquid chromatography with UV detection

Carlucci, Giuseppe; Mazzeo, Pietro; Biordi, Leda; Bologna, Mauro

doi:10.1016/0731-7085(92)80098-8

Cited by 75 publications

(39 citation statements)

References 3 publications

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“…Simvastatin is orally administered as inactive prodrug in the lactone form which is converted in the liver into its active metabolite β-hydroxy acid [3]. Recently, numerous methods for analysis of simvastatin and its metabolites determination in human plasma by HPLC methods with difference detectors (ultraviolet, fluorescence and mass spectrometry) have been published [4][5][6][7][8][9]. In addition, some methods of gas chromatography with mass spectrometry for determination of simvastatin and its metabolites have also been published [10][11].…”

Section: Introductionmentioning

confidence: 99%

Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)

Alakhali¹

2013

JCDR

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Introduction: The Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS) for determination simvastatin in human plasma has been developed after extraction by by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard. Material and Methods:The mobile phase consisting of mixture of acetonitrile and water (75/25%, v/v) 500μL/min by separated the solutes on a C18 column. discussion:The lower limit of quantitation of 0.25 ng/mL was achieved when the calibration curve was linear from 0.25-50 ng/mL. The entire run time for analysis was only 6 min. The quantitation in the selective reaction monitoring (SRM) in positive ion mode, the daughter ions m/z 325 for simvastatin and m/z 285 for lovastatin were used. The Parent ions in positive ion mode were m/z 441.3 for simvastatin and m/z 405.1 for lovastatin. The intra-day coefficients of variation were less than 14% while the inter-day coefficients of variation were less than 10%. conclusion:The LC-MS-MS detection is sensitive due to its capability to eliminate interferences from endogenous components.

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Section: Introductionmentioning

confidence: 99%

Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)

Alakhali¹

2013

JCDR

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“…Results obtained by the proposed procedures for the determination of SM and SIT in pure form and in pharmaceutical dosage form are statistically compared to those obtained by the reported methods [6,12]. The results showed no significant differences between them (Table 4).…”

Section: Discussionmentioning

confidence: 77%

“…The results obtained by those methods statistically compared by each other and by those obtained upon applying the reported methods [6,12] and no significant difference has been observed regarding to both accuracy and precision, Table 4.…”

Section: Pcr and Pls Modelsmentioning

confidence: 99%

“…Many techniques like UV-Vis spectrophotometry [5,6] and HPLC [7][8][9][10][11][12] have been reported for the determination of SM alone, in presence of its metabolites or in combination with other drugs. On the other hand, SIT was determined either alone or in presence of other drugs using different techniques like UV-Vis spectrophotometry [13], HPLC [14][15][16], and fluorescence spectroscopy [17].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of Simvastatin and Sitagliptin in tablets by new univariate spectrophotometric and multivariate factor based methods

2013

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KEYWORDSFive simple, sensitive and precise spectrophotometric and chemometric methods were used for simultaneous determination of Simvastatin (SM) and Sitagliptin (SIT) in their pure powdered forms and in the tablets. The proposed methods are the extended ratio subtraction method (EXRSM), ratio difference method (RDSM), mean centering of ratio spectra method (MCR) and chemometric methods, namely principal component regression (PCR) and partial least squares (PLS). In EXRSM; SM was determined at 237.5 nm, while SIT was determined at 267 nm, in RDSM; the difference in amplitudes at 237.5 and 245.5 nm was used for SM and 263.5 and 248.0 nm for SIT, while in MCR; SM and SIT were determined at 239.0 and 273.0 nm, respectively. PCR and PLS are factor based multivariate methods which utilize the whole spectra of SM and SIT. The developed methods were successfully applied for the determination of the studied drugs in their bulk powder, laboratory prepared mixtures and in tablets. All validation parameters of the developed methods were determined. The obtained results were statistically compared with each other along with a reported method.

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“…There are number of assay methods reported in the literature for simvastatin [18][19][20][21][22][23] and losartan [24][25][26][27] . Spectrophotometric techniques provide practical and significant economic advantages over other methods; therefore, they are a frequent choice for analyses.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Evidences for Simvastatin and Losartan Potassium Interaction and Its in Vivo Implications

Arayne

Sultana

Haroon

et al. 2009

J. Chil. Chem. Soc.

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In the present paper, we describe the in vitro availability of simvastatin, a potent HMG-CoA reductase inhibitor, in presence of losartan potassium, which is a non-peptide angiotensin II receptor antagonist. These studies were carried out at 37, 48 and 60°C in different pH environments simulating human body compartments. The reactions were studied by UV spectroscopy and the availability of both drugs in presence of each other was determined by deriving a simultaneous equation for two component system through modification of Beer`s law. The interactions were further characterized by IR and H 1 NMR spectroscopic techniques. It was observed that availability of simvastatin in presence of losartan at 37 ºC in simulated gastric juice, at pH 7.4 and pH 9.0 was blocked by losartan through the formation of a charge-transfer complex while the availability of losartan in turn was immensely increased. In pH 4.0 these effects were reversed, availability of simvastatin increased and the availability of losartan was depressed. Similar results were observed at higher temperatures.

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Simultaneous determination of simvastatin and its hydroxy acid form in human plasma by high-performance liquid chromatography with UV detection

Cited by 75 publications

References 3 publications

Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)

Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)

Simultaneous determination of Simvastatin and Sitagliptin in tablets by new univariate spectrophotometric and multivariate factor based methods

In Vitro Evidences for Simvastatin and Losartan Potassium Interaction and Its in Vivo Implications

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