Simultaneous determination of spirodiclofen, spiromesifen, and spirotetramat and their relevant metabolites in edible fungi using ultra-performance liquid chromatography/tandem mass spectrometry

Abstract

A fast, sensitive, and reliable analytical method was developed and validated for simultaneous identification and quantification of spirodiclofen, spiromesifen, and spirotetramat and their relevant metabolites in edible fungi by ultra-performance liquid chromatography/tandem mass spectrometry (UHPLC–MS/MS). First, sample extraction was done with acetonitrile containing 1% formic acid followed by phase separation with the addition of MgSO4:NaOAc. Then, the supernatant was purified by primary secondary amine (PSA), octadecylsilane (C18), and graphitized carbon black (GCB). The linearities of the calibrations for all analytes were excellent (R2 ≥ 0.9953). Acceptable recoveries (74.5–106.4%) for all analytes were obtained with good intra- and inter- relative standard deviations of less than 14.5%. The limit of quantification (LOQs) for all analytes was 10 μg kg−1. For accurate quantification, matrix-matched calibration curve was applied to normalize the matrix effect. The results indicated that the method was suitable for detecting the three acaricides and their relevant metabolites in edible fungi.

Development of a Quantitative Screening Method for 100 Pesticides in Plant-Derived Foods Using Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometry

“…In addition to PSA, C18, and GCB used in this study, some other adsorbents used for removing specific compounds also showed good purification effects. For example, the new materials EMR-lipid and LM806-FC-722 were applied to remove the fatty acids, lipids, and pigments from edible fungi . The results demonstrated that recovery and RSD were both satisfied when 20 mg of PSA + 30 mg of C18 and LM806-FC-722 were used in Pleurotus ostreatus.…”

“…For example, the new materials EMR-lipid and LM806-FC-722 were applied to remove the fatty acids, lipids, and pigments from edible fungi. 33 The results demonstrated that recovery and RSD were both satisfied when 20 mg of PSA + 30 mg of C18 and LM806-FC-722 were used in Pleurotus ostreatus. Meanwhile, 20 mg of PSA + 30 mg of C18 and EMR-lipid as sorbents were used in Flammulina velutiper, and the recoveries were satisfactory.…”

Experimental Study on the Degradation of Acaricides on the Surface of Kumquat Cuimi by Nonthermal Air Plasma

“…Spiromesifen is the most recently developed class of acaricides. These acaricides act as an acetyl-coenzyme A carboxylase (ACCase) inhibitor by interfering with lipid biosynthesis [5]. Etoxazole is a 2,4-diphenylthiazole derivative compound that can inhibit the embryonic formation of mite eggs and the molting process from larvae to adults.…”