“…It has the advantages of simple sample preparation, non-invasive, high automation, *weili@hust.edu.cn; phone 15337239902 high accuracy, fast speed, non-contact, etc. It has been widely used in fields such as biology and medicine, such as characterizing the size of bubbles in liquids 7 , measuring the characteristics of individual macromolecular solutes 8 , with a measurement range of 1nm~5μm. Applying DLS to particle size analysis2 of TO can supplement the shortcomings of light obscuration, extend the lower limit of particle size measurement to the submicron level, and quickly and accurately obtain particle size distribution.…”