Simultaneous determination of the vitamins A, E, their esters and coenzyme Q10 in multivitamin dietary supplements using an RP-C30 phase

Breithaupt, Dietmar E.; Kraut, Stefanie

doi:10.1007/s00217-005-0195-7

Cited by 22 publications

(18 citation statements)

References 14 publications

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“…Tocopherols were further identified by comparing retention times with those of authentic standards. The retention times of δ-, (β+γ)-and α-tocopherols were about 7.85 min, 8.65 min and 9.72 min., respectively and were determined from their authentic standards which were injected both individually and as a mixture .The analysis time is lower than found in the literature (Breithaupt and Kraut, 2006;Delgado-Zanarreno et al, 2006;Kuhn et al, 2008). Similar results were found by other authors with different protocols for sample preparation and HPLC separation of tocopherols (Chen et al, 2011;Gliszczynska-Swiglo and Sikorska, 2004).…”

Section: Methods Assessment and Validationsupporting

confidence: 76%

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Bele

Matea

Răducu

et al. 2013

Not Bot Hort Agrobot Cluj

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A quick and direct method based on reversed phase high performance liquid chromatography with fluorescence detector for measuring tocopherols (α , β + γ and δ) has been developed. Oils are diluted in methanol: hexane: tetrahydrofuran (neither previous extraction of tocopherols nor saponification procedure are required) and after being vortexed and centrifuged, an aliquot of the overlay was injected directly into an Alltima C 18 column. Acetonitrile and methanol (50: 50) mixture was used as a mobile phase with a flow rate of 1 mL min -1 . Quantification of tocopherols was performed by fluorescence detector at 290 nm excitation wavelength and 325 nm emission wavelength. Tocopherols were separated at 25°C in less than 10 min after injection. The method has good limit of detection (9 ng g -1 for α-tocopherol and 8 ng g -1 for β-, γ-and δ-tocopherols) and reproducibility (CV< 2.9 %). This method can be used to assess the influence of genetic modification of oil seeds on the distribution of tocopherols or the effect of tocopherols on the oxidative stability of edible plant oils.

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Section: Methods Assessment and Validationsupporting

confidence: 76%

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Bele

Matea

Răducu

et al. 2013

Not Bot Hort Agrobot Cluj

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“…The obtained run time is considerably shorter in comparison to other similar published HPLC-UV methods. [4][5][6]9,12 …”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

“…Due to the large number of commercially available vitamin preparations and their widespread use, the quality control of these preparations is extremely important, especially because of the risk of toxicity from excessive intake of vitamins A and D. 3 HPLC methods, coupled with different detection techniques: UV, [4][5][6][7][8][9][10][11][12] FLD, 6 ECD, 13 and MS 5,14 offer the best approach to accurate content determination of the main FSVs in foods, pharmaceuticals and nutritional supplements. The published HPLC-UV methods for quality control of preparations containing FSVs are quite limited in terms of separation and simultaneous determination of FSVs, 4,5,7,8,11 often have run time longer than 15 min [4][5][6]9,12 and generally require complicated and time-consuming sample preparation. 11,12,15,16 Further on, to our knowledge none of the published HPLC methods offer simultaneous determination of FSVs and also β-carotene and coenzyme Q10, which are often found along in medicines and nutritional supplements.…”

Section: Introductionmentioning

confidence: 99%

Novel HPLC-UV Method for Simultaneous Determination of Fat-soluble Vitamins and Coenzyme Q10 in Medicines and Supplements

Rakuša

Srečnik²,

Roškar

2017

ACSi

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A precise, accurate and rapid HPLC-UV method for simultaneous determination of fat-soluble vitamins (vitamin D3, E-acetate, K1, β-carotene, A-palmitate) and coenzyme Q10 was developed and validated according to ICH guidelines. Optimal chromatographic separation of the analytes in minimal analysis time (8 min) was achieved on a Luna C18 150 × 4.6 mm column using a mixture of acetonitrile, tetrahydrofuran and water (50:45:5, v/v/v). The described reversed phase HPLC method is the first published for quantification of these five fat-soluble vitamins and coenzyme Q10 within a single chromatographic run. The method was further applied for quantification of the analytes in selected liquid and solid dosage forms, registered as nutritional supplements and prescription medicines, which confirmed its suitability for routine analysis.

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“…Suplementos vitamínicos comerciais analisados quanto aos teores de vitaminas A e E, utilizando técnica de cromatografia líquida com detecção por arranjo de diodos e espectrometria de massas, mostraram resultados preocupantes, pois os valores verificados diferiram dos declarados no rótulo entre 46% e 245% 48 . No Brasil, não existem ainda análises confrontando os teores encontrados com os declarados nos rótulos de suplementos vitamínicos.…”

Section: Riscos à Saúdeunclassified

Suplementos vitamínicos e/ou minerais: regulamentação, consumo e implicações à saúde

2015

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Em decorrência das mudanças no padrão alimentar da população, a suplementação da dieta com micronutrientes é prática comum. A preocupação com a saúde e a facilidade de comercialização dos suplementos vitamínicos e/ou minerais, aliadas ao forte apelo publicitário, têm estimulado a população ao consumo indiscriminado desses produtos, o que pode acarretar riscos à saúde. Este trabalho teve como objetivo avaliar a legislação relativa ao cenário do consumo e segurança do uso de suplementos vitamínicos e/ou minerais no Brasil. Verificou-se que as legislações brasileiras a esse respeito são complexas, dificultando o entendimento das normas e a aplicação destas. Estudos sobre o consumo de suplementos pela população brasileira são limitados, e o consumo inadequado por falta de conhecimento é um potencial risco à saúde da população. Concluiu-se que há necessidade de implementação de políticas públicas que promovam o esclarecimento da população, dos profissionais da área de saúde e do comércio sobre o assunto.

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Simultaneous determination of the vitamins A, E, their esters and coenzyme Q10 in multivitamin dietary supplements using an RP-C30 phase

Cited by 22 publications

References 14 publications

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Novel HPLC-UV Method for Simultaneous Determination of Fat-soluble Vitamins and Coenzyme Q10 in Medicines and Supplements

Suplementos vitamínicos e/ou minerais: regulamentação, consumo e implicações à saúde

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