Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection

Mao-jin, Zhou; Chen, Xiaoyan; Zhong, Dafang

doi:10.1016/j.jchromb.2007.04.025

Cited by 22 publications

(13 citation statements)

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“…Using this method, the limit of detection for trans-resveratrol in this study was as low as 0.1 ng mL -1 , which is lower than the sensitivity reported by Juan et al [10] (250 ng mL -1 ), Chen et al [11] (66 ng mL -1 ), Wang et al [12] (35.5 ng mL -1 ), Boocock et al [13] (5 ng mL -1 ), Meng et al [14] (12.5 ng mL -1 ), He et al [15] (20 ng mL -1 ), Zhou et al [16] (40 ng mL -1 ), Huang et al [17] (58.5 ng mL -1 ), Juan et al [18] (2.3 ng mL -1 ) and Bernardes et al [19] (100 ng mL -1 ). In addition, this method requires only a very small amount of sample (100 lL), and the retention time of the method is 3.2 min compared with times of 8-25 min reported for other methods.…”

mentioning

confidence: 58%

“…Several extraction methods have been described, such as solid-phase extraction (SPE) [10,12,18], methanol [12,13] or acetonitrile [11,15,16] as protein precipitants, and liquid-liquid extraction with ethyl acetate [14,17]. In this study, ethyl acetate was used as the extraction solvent to obtain high sensitivity and avoid matrix interference.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

“…Currently, the primary focus of separation and quantification of trans-resveratrol in biological samples is based on the high-performance liquid chromatography with an ultraviolet detector (LC-UV) [10][11][12][13][14][15][16][17][18] and spectrofluorimetry [19]. The disadvantages of these methods are obvious: low selectivity, low sensitivity and long chromatographic running time.…”

mentioning

confidence: 99%

“…The disadvantages of these methods are obvious: low selectivity, low sensitivity and long chromatographic running time. Although the pharmacokinetics of trans-resveratrol have been described in humans [9,13] and rats [11,12,[15][16][17][18], few methods are available to evaluate the disposition of this drug at low blood levels. To elucidate the pharmacokinetics and pharmacodynamics of trans-resveratrol at low dosages, development of a sensitive and selective assay for the determination of trans-resveratrol in plasma is necessary.…”

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confidence: 99%

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Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS–MS

Hang

et al. 2011

Chromatographia

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A rapid, sensitive and highly selective liquid chromatography/tandem mass spectrometry (LC-MS-MS) using an atmospheric pressure chemical ionization (APCI) method was developed to determine the concentration of trans-resveratrol in rat plasma. Samples were extracted using ethyl acetate after daidzein was added as an internal standard (IS). The main advantages of this method were the high sensitivity (a lower limit of quantification, LLOQ, of 0.1 ng mL -1 ) and the short analysis time (total run time 3.2 min per sample). Separation was performed on a ZORBAX ODS column with a mobile phase that consisted of acetonitrile/water (48:52, v/v). trans-Resveratrol and IS were detected using a triple-quadrupole mass spectrometer in the selective reaction monitoring (SRM) mode with the parent-to-product quantifier transitions [M-H] -m/z 226.8 ? 142.9 and [M-H] -m/z 252.9 ? 252.9 for transresveratrol and IS, respectively. The method was confirmed to be accurate and precise with a linearity range of 0.1-500 ng mL -1 with r [ 0.99. Recoveries for transresveratrol and IS were within 90-101%. The accuracy and precision for the assay were determined by calculating the intra-and inter-batch variation for quality control (QC) samples at four concentration levels with a relative standard deviation (RSD) of \15%. This method was successfully applied to determine trans-resveratrol in rat plasma and proved suitable for pharmacokinetic study after intragastric administration of trans-resveratrol in sustained release granules and normal granules, respectively.

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Section: Methods Development and Optimizationmentioning

confidence: 99%

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confidence: 99%

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Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS–MS

Hang

et al. 2011

Chromatographia

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“…or water-miscible organic solvents [3][4][5][6][7][8][9]. Although all of these procedures can be used in discarding proteins prior to analysis…”

Section: Introductionmentioning

confidence: 99%

Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Alzweiri

Watson

Robertson

et al. 2008

Talanta

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This version is available at https://strathprints.strath.ac.uk/7602/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the AbstractThe Lowry method and a capillary electrophoresis method were used to analyse protein residues in the supernatant after solvent deproteination of plasma. Acetonitrile and acetone were much more effective than methanol and ethanol at reducing the levels of proteins in plasma. The ability of different solvents to decrease levels of phospholipids in plasma samples was assessed using electrospray ionisation mass spectrometry (MS). Phospholipid signals can obscure differences between samples in general metabolite profiling (i.e. non-target compound) studies. Acetonitrile was much more effective than methanol in reducing the MS signal due to phospholipids in plasma which is a consequence of the poor solubility of phospholipids in acetonitrile. The capability of the solvents at reducing salts in urine samples was also studied by using an amperometric method. Using this approach little difference was detected between methanol, ethanol, acetonitrile and acetone in their ability to desalt urine samples.

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Bioavailability and Metabolism of Resveratrol

Andrés‐Lacueva

Urpí-Sardà

Zamora-Ros

et al. 2009

Plant Phenolics and Human Health

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Simultaneous determination of trans-resveratrol-3-O-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection

Cited by 22 publications

References 19 publications

Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS–MS

Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS–MS

Comparison of different water-miscible solvents for the preparation of plasma and urine samples in metabolic profiling studies

Bioavailability and Metabolism of Resveratrol

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