Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

Jin, Miao; Song, Geng Shen; Du, Ying Feng; Cao, Liang; Xu, Hai; Zhang, Lan Tong

doi:10.1007/s10337-014-2840-4

Cited by 5 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was demonstrated that 23‐hydroxybetulinic acid induced apoptosis in HL‐60 cells via the downregulation of pro‐apoptotic gene bcl‐2 expression and telomerase activity as well as the upregulation of the apoptotic gene beclin‐1 expression (Ye & Ji, ). Anemoside B4, anemoside A3 and 23‐hydroxybetulinic acid have all been absolutely identified in the plasma and urine after the administration of P. chinensis saponins in rats (Jin et al, ; Xu et al, ), indicating that they can be absorbed into the circulatory system and then may play a role in the efficacy of P. chinensis . In addition, a developed and validated LC‐MS/MS method was applied for the determination of anemoside B4 over the concentration of 10–2000 ng/mL in various biological samples, and it showed that anemoside B4 elicited extensive tissue distribution after intravenous injection (He et al, ).…”

Section: Introductionmentioning

confidence: 99%

A sensitive HPLC–MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23‐hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

Xie

Lian

et al. 2017

Biomedical Chromatography

View full text Add to dashboard Cite

Pulsatilla chinensis saponins, the major active components in the herb, have drawn great attention as potential hepatitis B virus infection and hepatoma treatments. Here, a sensitive and accurate HPLC-MS/MS method was established for simultaneous determination of three saponins - anemoside B4, anemoside A3 and 23-hydroxybetulinic acid - in rat plasma and liver, and fully validated. The method was successfully applied to a pharmacokinetics and liver distribution study of P. chinensis saponins. Consequently, 23-hydroxybetulinic acid, with an extremely low content in the P. chinensis saponins, exhibited the highest exposure in the liver and in sites before and after hepatic disposition, namely, in the portal vein plasma and systemic plasma, followed by anemoside B4, which showed the highest content in the herb, whereas anemoside A3 displayed quite limited exposure. The hepatic first-pass effects were 71% for 23-hydroxybetulinic acid, 27% for anemoside B4 and 37% for anemoside A3, corresponding to their different extents of liver distribution. To our knowledge, this is the first investigation on the liver first-pass effect and distribution of P. chinensis saponins to date. These results also provide valuable information for the understanding of the pharmacological effect of P. chinensis saponins on liver diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

A sensitive HPLC–MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23‐hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

Xie

Lian

et al. 2017

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Recently, UHPLC–MS/MS has been widely accepted to be the predominant tool for qualitative and quantitative analysis of saponins in complex samples for it provides favorable specificity and sensitivity . In our previous study, we have established an HPLC–MS method for simultaneous determination of triterpenoid saponins in Pulsatilla turczaninovii and in rat urine after oral administration of P. chinensis extract. Compared to HPLC technique, UHPLC technique takes advantages of decreasing analysis time, extending sensitivity, and high solution.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 12 active components in the roots ofPulsatilla chinensisusing tissue-smashing extraction with liquid chromatography and mass spectrometry

Jin

Zhang

Xu³

et al. 2017

J. Sep. Sci.

Self Cite

View full text Add to dashboard Cite

Ultra high performance liquid chromatography coupled with mass spectrometry and combining a tissue-smashing extraction technique was developed for the simultaneous quantitative analysis of 12 compounds in the roots of Pulsatilla chinensis. Among them, compound 6 was characterized and accurately quantified in this herb for the first time. The parameters of extraction condition were simultaneously optimized with a Box-Behnken design and Derringer's function. The optimized conditions were as follows: sample quantity of 0.5 g, ethanol concentration of 70%, and extraction time of 200 s. Multiple-reaction monitoring scanning was employed for the quantification between positive and negative mode in a single run of 6 min. Full validation of the method was carried out, and the results indicated that the method was rapid, specific, and reliable. The developed method was successfully applied to quantify the 12 compounds in 33 batches of P. chinensis from different provinces. Moreover, the principal component analysis was performed to compare the P. chinensis collected from different provinces of China based on quantitative data and the results indicated that the content of compounds could be used to differentiate the origins of P. chinensis. These results demonstrated that this method is feasible and reliable for the quality control of P. chinensis.

show abstract

Polymer-Grafted Nanographite Support Obtained Using Iminodiacetic Acid/Allyl Glycidyl Ether: Characterization and Application in the Extraction and Determination of Enrofloxacin in Biological and Pharmaceutical Samples

Panahi

Alimadadi

2015

Chromatographia

View full text Add to dashboard Cite

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

Cited by 5 publications

References 14 publications

A sensitive HPLC–MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23‐hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

A sensitive HPLC–MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23‐hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

Simultaneous determination of 12 active components in the roots ofPulsatilla chinensisusing tissue-smashing extraction with liquid chromatography and mass spectrometry

Polymer-Grafted Nanographite Support Obtained Using Iminodiacetic Acid/Allyl Glycidyl Ether: Characterization and Application in the Extraction and Determination of Enrofloxacin in Biological and Pharmaceutical Samples

Contact Info

Product

Resources

About