Simultaneous Determination of Water‐Soluble Vitamins in Human Urine by Fluorescence in a Flow‐Injection Analysis

Wang, Lai‐Hao; Hung, Heng‐Chieh

doi:10.1080/10826070500451855

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…Other methods used to detect flavins include polarography [44, 45], flow injection analysis with fluorescence [46], square–wave voltammetry (SWV) using a mercury drop electrode [47], and spectrofluorimetry [48, 49]. Most of these methods require extracted liquid samples, and the analyzed data therefore represent the macroscale or bulk concentrations.…”

Section: Introductionmentioning

confidence: 99%

A voltammetric flavin microelectrode for use in biofilms

Nguyen

Renslow

Babauta

et al. 2012

Sensors and Actuators B: Chemical

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Biofilms used in bioelectrochemical systems are expected to transfer electrons using electron transfer mediators. One mediator type, flavins, which includes flavin mononucleotide, riboflavin, and flavin adenine dinucleotide, has been found to be endogenously produced by Shewanella oneidensis MR-1. However, the presence and concentration of flavins inside a S. oneidensis MR-1 biofilm have never been reported. The goal of this study was to develop a flavin microelectrode capable of measuring flavins inside a living biofilm and apply it to a biofilm which produces flavins. Because flavins are electrochemically active molecules, the flavin microelectrode was based on detection via square-wave voltammetry. The microelectrode consisted of a carbon working electrode with a 10–30 μm tip diameter, a built-in platinum counter electrode, and a Ag/AgCl reference electrode, all enclosed in a glass outer case. The microelectrode was calibrated between 0.1 μM and 10 μM flavins and showed a linear correlation between flavin concentration and peak currents located at −424 mVAg/AgCl on a square-wave voltammogram. We also developed a model to explain the electrochemical mechanism of flavin detection, and to determine the effective surface area of the microelectrode, the standard reduction potential, and the transfer coefficient. We found that the effective surface area of the microelectrode was close to 100 times the projected surface area. The model predicted a standard reduction potential for RF/RFH2 of −419 mVAg/AgCl at 20 °C and a transfer coefficient of 0.45. Lastly, we measured flavin concentration inside a S. oneidensis MR-1 biofilm grown on a glass surface using oxygen as the electron acceptor. The flavin concentration reached 0.7 μM, increasing near the bottom of the biofilm, where no oxygen was present. This shows the possibility that flavins are produced in the anaerobic zone to act as intermediate electron acceptors in the deeper parts of the biofilm, where there is no oxygen.

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Section: Introductionmentioning

confidence: 99%

A voltammetric flavin microelectrode for use in biofilms

Nguyen

Renslow

Babauta

et al. 2012

Sensors and Actuators B: Chemical

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“…As previously reported [11,12], RF in aqueous medium presents two excitation maxima at 8 approximately 368 and 440 nm, and a fluorescence emission maximum at 524 nm. The pH changes in the range 4-8 did not produce any significant modifications in the fluorescence signal [33].…”

Section: Preliminary Studiesmentioning

confidence: 64%

“…When RF intake is low, excretion is proportional to the intake [3]. The most widely used analytical methods for the quantification of RF in human urine samples are liquid chromatography (LC)-fluorescence detection (FD) [8], LC-tandem mass spectrometry (MS/MS) [9,10], fluorescence with flow-injection analysis [11], capillary electrophoresis (CE) [12,13], voltammetry [14] and chemiluminescence [15]. These methods require rigorous extraction steps, the use of significant amounts of organic solvents and long analysis time.…”

Section: Introductionmentioning

confidence: 99%

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation-emission fluorescence matrix (EEFM) data in standard addition mode and second-order chemometric analysis. The method is simple, fast and eco-friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial leastsquares/residual bilinearization (U-PLS/RBL) and multidimensional partial leastsquares/residual bilinearization (N-PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.

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“…In contrast, fluorometry is simple, sensitive and selective. Some fluorometric methods have already been reported in the literature for the determination of vitamin B 1 , most of which were mainly based on the fluorescent substance thiochrome, obtained by oxidizing vitamin B 1 with different oxidants (59,60,(69)(70)(71)(72)(73).…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of vitamin B₁using horseradish peroxidase as catalyst in the presence of hydrogen peroxide

et al. 2008

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In this work a new spectrofluorimetric method for the determination of vitamin B(1), based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2), has been developed. Non-fluorescent vitamin B(1) was easily converted through catalytic oxidation in alkaline medium into a fluorescent compound, even without exposure to light. The linear range for vitamin B(1) observed was 0.026-16.83 microg/mL (RSD = 1.75%). The correlation coefficient for the calibration curve and limit of detection were found to be 0.9964 and 0.015 microg/mL, respectively. The developed method is practical, simple, sensitive and relatively free from interference by coexisting substances and has been successfully applied for the determination of vitamin B(1) in pharmaceutical preparations.

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Simultaneous Determination of Water‐Soluble Vitamins in Human Urine by Fluorescence in a Flow‐Injection Analysis

Cited by 8 publications

References 19 publications

A voltammetric flavin microelectrode for use in biofilms

A voltammetric flavin microelectrode for use in biofilms

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Spectrofluorimetric determination of vitamin B₁using horseradish peroxidase as catalyst in the presence of hydrogen peroxide

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Simultaneous Determination of Water‐Soluble Vitamins in Human Urine by Fluorescence in a Flow‐Injection Analysis

Cited by 8 publications

References 19 publications

A voltammetric flavin microelectrode for use in biofilms

A voltammetric flavin microelectrode for use in biofilms

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Spectrofluorimetric determination of vitamin B1 using horseradish peroxidase as catalyst in the presence of hydrogen peroxide

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Spectrofluorimetric determination of vitamin B₁using horseradish peroxidase as catalyst in the presence of hydrogen peroxide