Simultaneous determination of γ-aminobutyric acid and glutamic acid in the brain of 3-mercaptopropionic acid-treated rats using liquid chromatography–atmospheric pressure chemical ionization mass spectrometry

Ma, Deshun; Zhang, Jianying; Sugahara, Kazunori; Ageta, Tomiko; Nakayama, Kazunori; Kodama, Hiroyuki

doi:10.1016/s0378-4347(99)00025-0

Cited by 21 publications

(14 citation statements)

References 18 publications

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“…However, this result does not question the identification of Glu in the microdialysates, but rather confirms the tight metabolic link between GABA and Glu [32]. In this respect, MPA-induced changes in Glu have previously been reported [33]. Finally, the relative areas of the peak with the same migration time as L-Asp standard exhibited changes similar to those of Glu, since it was only affected by Glu/Asp uptake blocker (data not shown).…”

Section: In Vivo Pharmacological Validationsupporting

confidence: 81%

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

et al. 2003

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gamma-Aminobutyric acid (GABA), glutamate (Glu), and L-aspartate (L-Asp) are three major amino acid neurotransmitters in the central nervous system. In this work, a method for the separation of these three neurotransmitters in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. Molecules were tagged on their primary amine function with the fluorogene agent naphthalene-2,3-dicarboxaldehyde (NDA), and, after separation by micellar electrokinetic chromatography, were detected by laser-induced fluorescence using a 442 nm helium-cadmium laser. The separation conditions for the analysis of derivatized neurotransmitters in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical basis. The separation of GABA, Glu, and L-Asp takes less than 10 min by using a 75 mmol/L borate buffer, pH 9.2, containing 70 mmol/L SDS and 10 mmol/L hydroxypropyl-beta-cyclodextrin and + 25 kV voltage. The detection limits were 3, 15 nmol/L and, 5 nmol/L for GABA, Glu, and L-Asp, respectively. Moreover, submicroliter samples can be analyzed. This method allows a simple, rapid and accurate measurement of the three amino acid neurotransmitters for the in vivo brain monitoring using microdialysis sampling.

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Section: In Vivo Pharmacological Validationsupporting

confidence: 81%

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

et al. 2003

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“…However, this result does not question the identification of Glu in the microdialysates, but rather confirms the tight metabolic link between GABA and Glu [24]. In this respect, mercaptopropionic acid-induced changes in Glu have previously been reported [18,25]. The linearity and repeatability were determined for Glu, l-Asp, GABA and vigabatrin, contained in standard solutions as well as in brain microdialysates ( Table 1).…”

Section: Methods Validationssupporting

confidence: 66%

Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection

Benturquia

Parrot

Sauvinet

et al. 2004

Journal of Chromatography B

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“…This incubation time was regarded as sufficient on the basis of a previously reported turnover time (10-14min) of GABA in mice (Gomes and Trolin, 1982). The inhibition of GABA synthesis by the inhibition of GAD with 3-MPA (Ma et al, 1999) was confirmed by whole-mount IHC. The larvae collected from the bottom 500μl of each tube using Pasteur pipettes (bottom fraction) represented 'poor swimmers'.…”

Section: Swimming Assay Of Larvae Treated With 3-mpa or Bicucullinementioning

confidence: 94%

Development of the γ-amino butyric acid (GABA)-ergic signaling system and its role in larval swimming in sea urchin

Katow

Abe

Katow

et al. 2013

Journal of Experimental Biology

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SUMMARYThe present study aimed to elucidate the development and γ-amino butyric acid (GABA)-ergic regulation of larval swimming in the sea urchin Hemicentrotus pulcherrimus by cloning glutamate decarboxylase (Hp-gad), GABA A receptor (Hp-gabrA) and GABA A receptor-associated protein (Hp-gabarap), and by performing immunohistochemistry. The regulation of larval swimming was increasingly dependent on the GABAergic system, which was active from the 2days post-fertilization (d.p.f.) pluteus stage onwards. GABA-immunoreactive cells were detected as a subpopulation of secondary mesenchyme cells during gastrulation and eventually constituted the ciliary band and a subpopulation of blastocoelar cells during the pluteus stage. Hp-gad transcription was detected by RT-PCR during the period when Hp-Gad-positive cells were seen as a subpopulation of blastocoelar cells and on the apical side of the ciliary band from the 2d.p.f. pluteus stage. Consistent with these observations, inhibition of GAD with 3-mercaptopropioninc acid inhibited GABA immunoreactivity and larval swimming dose dependently. Hp-gabrA amplimers were detected weakly in unfertilized eggs and 4d.p.f. plutei but strongly from fertilized eggs to 2d.p.f. plutei, and Hp-GabrA, together with GABA, was localized at the ciliary band in association with dopamine receptor D1 from the two-arm pluteus stage. Hpgabarap transcription and protein expression were detected from the swimming blastula stage. Inhibition of the GABA A receptor by bicuculline inhibited larval swimming dose dependently. Inhibition of larval swimming by either 3-mercaptopropionic acid or bicuculline was more severe in older larvae (17 and 34d.p.f. plutei) than in younger ones (1d.p.f. prism larvae).

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Simultaneous determination of γ-aminobutyric acid and glutamic acid in the brain of 3-mercaptopropionic acid-treated rats using liquid chromatography–atmospheric pressure chemical ionization mass spectrometry

Cited by 21 publications

References 18 publications

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

In vivo simultaneous monitoring of γ‐aminobutyric acid, glutamate, and L‐aspartate using brain microdialysis and capillary electrophoresis with laser‐induced fluorescence detection: Analytical developments and in vitro/in vivo validations

Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection

Development of the γ-amino butyric acid (GABA)-ergic signaling system and its role in larval swimming in sea urchin

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