Simultaneous dispersion–dissolution behavior of concentrated silver nanoparticle suspensions in the presence of model organic solutes

Chappell, Mark A.; Miller, Lesley F.; George, Aaron J.; Pettway, Brad A.; Price, Cynthia B.; Porter, Beth E.; Bednar, Anthony J.; Seiter, Jennifer M.; Kennedy, Alan J.; Steevens, Jeffery A.

doi:10.1016/j.chemosphere.2011.04.040

Cited by 52 publications

(50 citation statements)

References 43 publications

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“…The surface charge can influence nanoparticle interactions with living systems, and thus, their toxicity, and may change depending on the dispersant. 59 Taking the literature into account, we were interested in studying the cytotoxicity of B-AgNPs and F-AgNPs derived from bacterial culture supernatant and milky mushroom extract, respectively, as a reducing agent. Therefore, we were interested in investigating whether AgNPs derived from two different capping agents and whether different shapes of AgNPs could influence cell viability with remarkable effect.…”

Section: Cytotoxicity Of B-agnps and F-agnpsmentioning

confidence: 99%

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

275

151

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Background Recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. This is especially true in the area of nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. The first goal of this study was to produce silver nanoparticles (AgNPs) using two different biological resources as reducing agents, Bacillus tequilensis and Calocybe indica. The second goal was to investigate the apoptotic potential of the as-prepared AgNPs in breast cancer cells. The final goal was to investigate the role of p53 in the cellular response elicited by AgNPs. Methods The synthesis and characterization of AgNPs were assessed by various analytical techniques, including ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). The apoptotic efficiency of AgNPs was confirmed using a series of assays, including cell viability, leakage of lactate dehydrogenase (LDH), production of reactive oxygen species (ROS), DNA fragmentation, mitochondrial membrane potential, and Western blot. Results The absorption spectrum of the yellow AgNPs showed the presence of nanoparticles. XRD and FTIR spectroscopy results confirmed the crystal structure and biomolecules involved in the synthesis of AgNPs. The AgNPs derived from bacteria and fungi showed distinguishable shapes, with an average size of 20 nm. Cell viability assays suggested a dose-dependent toxic effect of AgNPs, which was confirmed by leakage of LDH, activation of ROS, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells in MDA-MB-231 breast cancer cells. Western blot analyses revealed that AgNPs induce cellular apoptosis via activation of p53, p-Erk1/2, and caspase-3 signaling, and downregulation of Bcl-2. Cells pretreated with pifithrin-alpha were protected from p53-mediated AgNPs-induced toxicity. Conclusion We have demonstrated a simple approach for the synthesis of AgNPs using the novel strains B. tequilensis and C. indica , as well as their mechanism of cell death in a p53-dependent manner in MDA-MB-231 human breast cancer cells. The present findings could provide insight for the future development of a suitable anticancer drug, which may lead to the development of novel nanotherapeutic molecules for the treatment of cancers.

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Section: Cytotoxicity Of B-agnps and F-agnpsmentioning

confidence: 99%

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

275

151

View full text Add to dashboard Cite

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“…Similarly, Gao et al (2012) reported that AgNPs (unspecified coating) were stabilized with HA (<10 mg/L), whereas higher HA concentrations (>10 mg/L) induced aggregation. Other studies that addressed the behavior of AgNPs in natural waters in the presence and absence of humic materials and other organic solutes showed condition-and coating-dependent agglomeration or stabilization of AgNPs (Chappell et al, 2011;Li and Lenhart, 2012;Piccapietra et al, 2012). Moreover, two recent studies reported that AgNPs can be regenerated through sunlightdriven reduction of Ag + by organic matter (Hou et al, 2013;Yu et al, 2014), whereas George et al (2014) reported that solar light increased AgNP dissolution and thus its toxicity to a fish cell line and zebrafish embryos further emphasizing the importance of environmental factors in determining the fate of AgNPs.…”

Section: Environmental Fate Of Agnpsmentioning

confidence: 98%

Predicting the environmental impact of nanosilver

Massarsky

Trudeau

Moon

2014

Environmental Toxicology and Pharmacology

126

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“…The most effective approach is nanoparticle surface functionalization to provide hydrophobic functional groups that can mix homogeneously with hydrophobic liquid media, such as organic solvents, and thus readily integrate with the dissolved polymer chains [25][26][27]. Recent work with hybrid materials highlights the importance of organic dispersants as the anchoring and binding species for the nanoparticles and polymers [26,[28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

Liu

Gervasio

2015

J Mater Sci

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Aqueous nanoparticle dispersions have been widely studied for their important industrial applications, including photocatalyst, coating, and casting. However, much less work has been conducted on nanoparticle dispersions in organic solvents, especially when co-dispersing with dissolved polymers. In this study, we explore TiO 2 / ZnO nanoparticle and poly(methyl methacrylate) (PMMA) co-dispersion in organic solvent anisole. To achieve uniformly dispersed suspensions, nanoparticle surface functionalization with nonanoic acid and 1-pentanol is conducted. Nonanoic acid has higher surface coverage on TiO 2 and the two dispersants show similar surface coverage on ZnO. Heat treatment enhances the dispersant adsorption on the nanoparticle surfaces. For both TiO 2 / PMMA and ZnO/PMMA co-dispersions, higher solids loading leads to higher suspension viscosity. Higher volume fraction of nanoparticles in the nanoparticle plus PMMA solid phase results in lower viscosity, mainly by reducing the entanglement of the dissolved PMMA chains.

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Simultaneous dispersion–dissolution behavior of concentrated silver nanoparticle suspensions in the presence of model organic solutes

Cited by 52 publications

References 43 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Predicting the environmental impact of nanosilver

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

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Simultaneous dispersion–dissolution behavior of concentrated silver nanoparticle suspensions in the presence of model organic solutes

Cited by 52 publications

References 43 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Predicting the environmental impact of nanosilver

Nanoparticle and poly(methyl methacrylate) co-dispersion in anisole

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Product

Resources

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Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy