Simultaneous enzymatic hydrolysis and anaerobic biodegradation of lipid-rich wastewater from poultry industry

Abstract

Simultaneous enzymatic hydrolysis and anaerobic biodegradation of lipid-rich wastewater from poultry industry with porcine pancreatic lipase at different concentrations (from 1.0 to 3.0 g L-1) were performed. The efficiency of the enzymatic pretreatment was measured by the Chemical Oxygen Demand (COD) removal and formation of methane. All samples pretreated with lipase showed a positive effect on the COD removal and formation of methane. After 30 days of anaerobic biodegradation the methane production varied from 569 ± 95 to 1,101 ± 10 mL for crude wastewater and pretreated at 3.0 g L-1 enzyme, respectively. COD removal of wastewater supplemented at different enzyme concentrations was found to be threefold higher than crude wastewater. The use of lipases seems to be a promising alternative for treating lipid-rich wastewaters such as those from the poultry industry.

Fosfolipase imobilizada na hidrólise da fração lipídica de efluente de frigorífico de suínos: comparação com a enzima livre

“…Segundo Rigo et al (2008), valores de DQO para efluente de frigoríficos podem chegar a 4.500 mg O 2 .L -1 , porém Dors et al (2013) e Valladão, Freire, Cammarota (2007), ao caracterizarem efluente de frigorífico de aves, obtiveram valores de 2.005 mg O 2 .L -1 e 1.181 mg O 2 .L -1 , demostrando que esses valores podem variar em uma ampla faixa. Os teores de óleos e graxas também variam, sendo citados na literatura os valores de 8.462 mg.L -1 para efluente e frigorífico de suínos e bovinos pré-flotado (RIGO et al, 2008) Segundo Won et al (2005), o aprisionamento é uma das técnicas de imobilização definidas como uma restrição física da enzima dentro de um espaço confinado, ou de uma rede que permite a transferência de massa.…”

Treatment of Wastewater With High Fat Content Employing an Enzyme Pool and Biosurfactant: Technical and Economic Feasibility

“…However, no significant differences were found between reactors fed with pre-hydrolyzed wastewater and reactors fed with unhydrolyzed wastewater in the treatment of a slaughterhouse wastewater containing 550 mg/L of fat. Dors et al (2013) studied the addition of commercial pancreatic lipases on biodegradability of wastewater from a poultry processing industry. Enzymatic treatments were performed by varying the enzyme concentrations from 1 to 3 g/L.…”

Rhodococcus UKMP-5M, an endogenous lipase producing actinomycete from Peninsular Malaysia

“…Commonly, partial recovery of lipid residues will be done through air floatation where floated lipid is discarded by sanitary landfill dumping prior to normal water treatment (Mongkolthanaruk & Dharmsthiti 2002). Anaerobic water treatment on the other hand, imposed number of limitations including clogging on digesters, mass transfer defects for soluble substrates, floatation of biomass and severe lipid toxicity against both methanogenic and acetogenic microbes (Cirne et al 2007;Dors et al 2013). Meanwhile, other applicable alternative treatment includes thermochemical, alkaline and enzymatic pre-treatment.…”

“…Considering the necessity of improvement in biological treatment, enzymatic technology especially with regard to lipase is gaining increasing attention (Whiteley & Lee 2006;Dors et al 2013). Aerobic biodegradation processes by active lipolytic strains are regarded as slower and less viable on highly polluted fat contaminants.…”

“…It was understood that aerobic biological treatment also releases a substantial amount of energy which can be utilized by bacteria for maximal synthesis and growth of new cells (Eckenfelder et al 1988;Chan et al 2008). Enzymatic pre-treatment have been labelled as efficient due to stringent environmental regulations, clean and eco-friendly applications which effectually decreases chemical oxygen demand, colour, and suspended lipid solids (Dors et al 2013). Few studies on lipase are effective for food waste treatment, such as study by Prasad & Manjunath (2011) who reported high removal of lipid content and reduced level of biochemical oxygen demand by several lipase producing bacteria on dairy effluents and slaughterhouses wastewater aerobically.…”