Simultaneous Estimation of Amlodipine and Atenolol in Human Plasma: A Sensitive LC–MS/MS Method Validation and its Application to A Clinical PK Study

Abstract

The intra- and inter-day accuracy and precision values for AMD and ATL met the acceptance as per regulatory guidelines. The validated assay was applied to a fixed-dose combination of AMD and ATL (Adopin-AT(®)) PK study in humans.

An Improved Rapid HPLC Method for the Separation of Five Anti-Hypertensive Agents Using C18 Column: Application to Hydrochlorothiazide Determination in Bulk and Tablet Dosage Form

“…Literature survey reveals that for the determination of above said five anti-hypertensive agents in biological fluids like plasma, blood, urine and pharmaceutical dosage forms by spectrophotometry [1][2][3][4] , spectrofluorimetry [5] , TLC [6][7] , HPTLC [8] , High Performance Liquid Chromatography (RP-HPLC) with UV detection [9][10][11][12][13][14][15][16][17][18] , fluorimetric detection [19] , ion-pair HPLC [20] , RP-UPLC [21] and Liquid Chromatography-Mass Spectrometry [22][23] , Capillary electrophoresis [24] .In fact there is a need for the development of a novel, simple, rapid, efficient RP-HPLC analytical method with reproducibility for determination of HCT in bulk and pharmaceutical dosage forms. A group of five anti-hypertensive drugs investigated in the present study are listed in Figure 1.…”

Detection of vasodilator drugs through microwave spectroscopy of Au NP colorimetric probes using a microwave metallic photonic crystal-inspired resonant probe

“…3 Minoxidil is used to control blood pressure by relaxing and dilating the blood vessels, and it is always used in combination with beta-blockers and diuretics in order to prevent its side effects. 4 Several analytical techniques such as High Pressure Liquid Chromatography (HPLC), 5,6 Liquid Chromatography-Mass Spectrometry (LC-MS), 7,8 uorescence, 9,10 electrochemical, 11,12 and UV-visible spectroscopy 13,14 are reported for the analysis of two vasodilator drugs (amlodipine besylate and minoxidil) in pure pharmaceutical formulations and tablets. Unfortunately, these methods require more time for analysis, expensive instrumentation, and moreover, their applicability is limited only to the laboratory.…”

Simultaneous determination of rosuvastatin and amlodipine in human plasma using tandem mass spectrometry: Application to disposition kinetics

“…The major disadvantages of these methods include, less sensitivity [11] , more sample volume (>0.25 mL) [11] , [13] , [14] , [19] , longer chromatographic run time (>4 min) [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , complex with derivatization and expensive automated extraction procedure [13] , [18] , and narrow linearity range not suitable for bioequivalence/pharmacokinetic application in humans at higher dose (0.1–30 ng/mL) [13] , [14] . Similarly, numerous LC/MS/MS methods are described in the literature to determine amlodipine in different biological fluids [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] . Among the applied methods, either the chromatographic run time was long (>4 min) [19] , [20] , [22] , [24] , [25] , [30] , [31] , the plasma volume was high (>0.25 mL) [19] , [21] , [22] , [23] , [24] , [25] , [30] or the method was insensitive for bioequivalence/pharmacokinetic application [20] , [23] , [25] , [27] , [30] , [31] .…”

“…Some methods [15] , [21] , [26] , [27] , [28] , [29] which can be applied for quantitation of one drug in biological fluids selectively and sensitively, cannot be applied satisfactorily for simultaneous determination of rosuvastatin and amlodipine. To investigate the safety and tolerability of rosuvastatin and amlodipine fixed dose combination (FDCs) and/or for comparative bioavailability and bioequivalence studies of rosuvastatin associated with amlodipine, it is necessary to perform the quantitation of rosuvastatin and amlodipine simultaneously.…”