Simultaneous Estimation of Diphenoxylate HCL and Atropine Sulphate in Solid Dosage Forms by High Performance Liquid Chromatography

Niaz, Hadia; Hassan, Syed Rizwan; Iqbal, Muhammad Khalid; Ahmed, Hammad; Jamshaid, Tahir; Rehman, Muhammad Khalil Ur

doi:10.1007/s11094-021-02525-7

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Various methods using different detection systems have been used to determine diphenoxylate, including capillary electrophoresis [23], mass spectrometry [22], high-performance liquid chromatography [24][25][26][27], spectrophotometry [28], and immunoassay [29]. To our knowledge, this is the first CL method proposed for diphenoxylate determination.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

Koohsarian,

Mokhtari,

Hooshmand

2024

Luminescence

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In this study, a chemiluminescence (CL) method was developed to determine diphenoxylate in tablets and human plasma. This is the first CL method proposed to determine diphenoxylate. Creating three‐dimensional data caused the parallel factor analysis algorithm (PARAFAC) to be used for the first time in CL methods. The method is based on the fact that diphenoxylate enhances the weak CL produced in the reaction of Ru(phen)32+ and acidic Ce(IV), and the concentration of Ce(IV) solution has a different effect on the CL response of diphenoxylate and the blank plasma. The calibration curve was linear from 4.0 × 10−8 to 1.6 × 10−6 mol L−1 (R2 = 0.9954), and the detection limit was 1.3 × 10−8 mol L−1 (S/N = 3). The sampling rate was about 30 samples per hour, and the % RSD for 10 repeated measurements of 4 × 10−7 mol L−1 diphenoxylate was 5.4%. The interference effects of some ions, amino acids, and common additives were also investigated. The CL method was successfully used to determine diphenoxylate in tablets, and the results were statistically confirmed by the reference method. The proposed CL method and the PARAFAC algorithm were successfully used to determine the concentration of diphenoxylate in human blood plasma samples.

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Section: Introductionmentioning

confidence: 99%

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

Koohsarian,

Mokhtari,

Hooshmand

2024

Luminescence

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“…Both medical and pharmacological uses rely on the determination of atropine. The basic methods to determine atropine are chromatographic separation methods [56][57][58]. These processes are considered as high-cost tools and necessitate the use of an extraction solvent.…”

Section: Introductionmentioning

confidence: 99%

Atropine-Phosphotungestate Polymeric-Based Metal Oxide Nanoparticles for Potentiometric Detection in Pharmaceutical Dosage Forms

et al. 2022

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The new research presents highly conductive polymeric membranes with a large surface area to volume ratio of metal oxide nanoparticles that were used to determine atropine sulfate (AT) in commercial dosage forms. In sensing and biosensing applications, the nanomaterials zinc oxide (ZnONPs) and magnesium oxide (MgONPs) were employed as boosting potential electroactive materials. The electroactive atropine phosphotungstate (AT-PT) was created by combining atropine sulfate and phosphotungstic acid (PTA) and mixing it with polymeric polyvinyl chloride (PVC) with the plasticizer o-nitrophenyl octyl ether (o-NPOE). The modified sensors AT-PT-ZnONPs or AT-PT-MgONPs showed excellent selectivity and sensitivity for the measurements of atropine with a linear concentration range of 6.0 × 10−8 − 1.0 × 10−3 and 8.0 × 10−8 − 1.0 × 10−3 mol L−1 with regression equations of E(mV) = (56 ± 0.5) log [AT] − 294 and E(mV) = (54 ± 0.5) log [AT] − 422 for AT-PT-NPs or AT-PT-MgONPs sensors, respectively. The AT-PT coated wire sensor, on the other hand, showed a Nernstian response at 4.0 × 10−6 − 1.0 × 10−3 mol L−1 and a regression equation E(mV) = (52.1 ± 0.2) log [AT] + 198. The methodology-recommended guidelines were used to validate the suggested modified potentiometric systems against various criteria.

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Simultaneous Estimation of Diphenoxylate HCL and Atropine Sulphate in Solid Dosage Forms by High Performance Liquid Chromatography

Cited by 2 publications

References 13 publications

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

Atropine-Phosphotungestate Polymeric-Based Metal Oxide Nanoparticles for Potentiometric Detection in Pharmaceutical Dosage Forms

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