Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection

Potěšil, David; Petrlová, Jitka; Adam, Vojtěch; Vacek, Jan; Klejdus, Bořivoj; Zehnálek, Josef; Trnková, Libuše; Havel, Ladislav; Kizek, René

doi:10.1016/j.chroma.2005.06.019

Cited by 175 publications

(96 citation statements)

References 61 publications

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“…GSH concentration was calculated from a calibration curve (0.5-100 µM). The signal was quantified as a sum of current responses from all working electrodes (54,55). In the case of real sample measurements, the shift of the retention time was of about ±2%.…”

Section: Methodsmentioning

confidence: 99%

Caveolin-1 as a potential high-risk prostate cancer biomarker

Gumulec

Sochor²,

Hlavna³

et al. 2011

Oncol Rep

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Abstract. Current diagnostic techniques of prostate cancer cannot efficiently distinguish the latent and low-risk forms from the high-risk significant forms of prostate cancer. Caveolin-1 (Cav-1), except other functions, plays an important role in cell transformation and the process of tumorigenesis. Furthermore, Cav-1 is involved in metastatic processes. It has also been shown that Cav-1 expression is induced under stress conditions, such as oxidative stress. The present study focused on the determination of prognostic markers of aggressive (high-grade) forms of prostate cancer. We determined serum Cav-1 and serum markers of antioxidant activity-glutathione (GSH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), ferric-reducing antioxidant power (FRAP), N,N-dimethyl-1,4-diaminobenzene (DMPD), free radicals method (FRK) and blue chromium peroxide (Cro) in 97 serum samples (82 prostate cancer patients and 15 controls). We found insignificant differences in Cav-1 between the sera of patients and controls (5.69 in the cancer group vs. 5.42 ng/ml in the control group). However, we found a significant (p<0.004) 2.8-fold elevation of Cav-1 in high tumour stages (TNM T4) compared to lower stages and a significant positive association with histological grading (r= 0.29, p= 0.028). We also found that in patients with high serum Cav-1 the antioxidant capacity of the body is reduced. These findings indicate that Cav-1 may be an interesting tool for the prediction of disease burden.

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Section: Methodsmentioning

confidence: 99%

Caveolin-1 as a potential high-risk prostate cancer biomarker

Gumulec

Sochor²,

Hlavna³

et al. 2011

Oncol Rep

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“…For many years, GSH has been measured by several analytical methods. In particular, high performance liquid chromatography (HPLC) with various detection techniques including ultraviolet (UV) absorbance and fluorescence detection, mass spectrometry and/or electrochemical detection (ED) are commonly used for determination of GSH and GSSG concentrations (46)(47)(48)(49). Each method has its advantages and limitations and may serve a particular need in analysis (50).…”

Section: Introductionmentioning

confidence: 99%

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

et al. 2012

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Abstract.Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, and it is involved in numerous malignancies. Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) may be used as a marker of oxidative stress. The main aim of this study was to determine GSH:GSSG ratio in the blood serum of paediatric cancer patients to use this ratio as a potential marker of oxidative stress. The whole procedure was optimised and the recoveries for both substances were greater than 80% under the optimised conditions. We analysed a group of paediatric patients (n=116) with various types of cancer, including neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract tumour, lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma and retinoblastoma. We simultaneously determined the levels of reduced and oxidised glutathione, and thus, its ratio in the blood serum of the patients. The highest ratio was observed in retinoblastoma patients and the lowest in anaplastic ependymoma. We were able to distinguish between the diagnoses based on the results of the obtained GSH:GSSG ratio.

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“…PCs have a basic formula (γ-Glu-Cys)n-Gly (n = 2 to 11) and form M-PC complexes with the heavy metals (M), in which the metal is bound via the cysteine unit SH group [1,6,7]. A very wide range of analytical techniques, including both chromatographic coupled with different detectors and stationary ones, such as electrochemical methods, have been utilized for determination of thiols in samples of interest [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Šupálková

Petřek

Baloun

et al. 2007

Sensors

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Abstract:The main aim of this work was to use multi-instrumental analytical apparatus to investigate the effects of treatment with cadmium(II) and/or lead(II) ions (50, 250 and 500 µM) for twelve days on early somatic spruce embryos (ESEs). Primarily we used image analysis for estimation of growth and a fluorimetric sensor for enzymatic detection of viability of the treated ESEs. It follows from the obtained results that Cd caused higher toxicity to ESEs than Pb. Besides this fundamental finding, we observed that ESEs grew and developed better in the presence of 500 µM of the metal ions than in the presence of 250 µM. Based on the results obtained using nuclear magnetic resonance this phenomenon was related to an increase of the area of ESE clusters by intensive uptake of water from the cultivation medium, due to dilution of the heavy metal concentration inside the cluster. In addition we studied the glutathione content in treated ESEs by the adsorptive transfer stripping technique coupled with the differential pulse voltammetry Brdicka reaction. GSH contents increased up to 148 ng/mg (clone 2/32) and 158 ng/mg (clone PE 14) after twelve Sensors 2007, 7 744 day long treatment with Cd-EDTA ions. The GSH content was about 150 and 160 % higher in comparison with the ESEs treated with Pb-EDTA ions, respectively. The difference between GSH contents determined in ESEs treated with Pb-EDTA and Cd-EDTA ions correlates with the higher toxicity of cadmium(II) ions.

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Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection

Cited by 175 publications

References 61 publications

Caveolin-1 as a potential high-risk prostate cancer biomarker

Caveolin-1 as a potential high-risk prostate cancer biomarker

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

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