Simultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE

Guerfali, Fatma Z.; Laouini, Dhafer; Guizani-Tabbane, Lamia; Ottonès, Florence; Ben-Aissa, Khadija; Benkahla, Alia; Manchon, Laurent; Piquemal, David; Smandi, Sondos; Mghirbi, Ons; Commes, Thérèse; Marti, Jacques; Dellagi, Koussay

doi:10.1186/1471-2164-9-238

Cited by 57 publications

(64 citation statements)

References 50 publications

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“…Dual-transcriptome studies of microbes and their hosts have been used to analyze both pathogenic (20) and, more rarely, beneficial associations (21-23). We report here determinations of synchronous gene-expression patterns for both partners of a symbiosis over the course of a day-night cycle.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Wier¹,

Nyholm

Mandel³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

152

221

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Mechanisms for controlling symbiont populations are critical for maintaining the associations that exist between a host and its microbial partners. We describe here the transcriptional, metabolic, and ultrastructural characteristics of a diel rhythm that occurs in the symbiosis between the squid Euprymna scolopes and the luminous bacterium Vibrio fischeri. The rhythm is driven by the host's expulsion from its light-emitting organ of most of the symbiont population each day at dawn. The transcriptomes of both the host epithelium that supports the symbionts and the symbiont population itself were characterized and compared at four times over this daily cycle. The greatest fluctuation in gene expression of both partners occurred as the day began. Most notable was an up-regulation in the host of >50 cytoskeleton-related genes just before dawn and their subsequent down-regulation within 6 h. Examination of the epithelium by TEM revealed a corresponding restructuring, characterized by effacement and blebbing of its apical surface. After the dawn expulsion, the epithelium reestablished its polarity, and the residual symbionts began growing, repopulating the light organ. Analysis of the symbiont transcriptome suggested that the bacteria respond to the effacement by up-regulating genes associated with anaerobic respiration of glycerol; supporting this finding, lipid analysis of the symbionts' membranes indicated a direct incorporation of host-derived fatty acids. After 12 h, the metabolic signature of the symbiont population shifted to one characteristic of chitin fermentation, which continued until the following dawn. Thus, the persistent maintenance of the squid-vibrio symbiosis is tied to a dynamic diel rhythm that involves both partners.Euprymna scolopes | microarray | mutualism | Vibrio fischeri | cytoskeleton

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Section: Discussionmentioning

confidence: 99%

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Wier¹,

Nyholm

Mandel³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

152

221

View full text Add to dashboard Cite

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“…Extensive manipulations of host cell effector (innate and adaptive) functions by pathogens must be reflected at the levels of transcripts as well as proteins. Enormous efforts made in the field of host gene expression profiling using different (murine and/or human) cell types and different species of Leishmania provide key insights into an extensive modulation of gene function and contribute to a better understanding of the dynamics of gene expres-sion postinfection (11)(12)(13)(14). This type of transcriptome-based analysis has major limitations, as it does not represent the true effectors of cellular functions-the proteins.…”

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confidence: 99%

Proteomic-Based Approach To Gain Insight into Reprogramming of THP-1 Cells Exposed to Leishmania donovani over an Early Temporal Window

et al. 2015

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Leishmania has developed an intricate relationship with its host, primarily cells of the monocyte/macrophage lineage, where it exploits and subverts the host immune system by either inducing immunosuppression or promoting proparasitic host factors to ensure its survival and growth in an otherwise harsh milieu (3). Hijacking of innate immune functions of macrophages by Leishmania appears to be a multifarious event, as macrophages have inherently evolved to defend the host against invading pathogens by a myriad of effectors rather than providing a favorable environment to the pathogen. The chief molecular mechanisms by which Leishmania is known to inhibit the activation of macrophages toward its own benefit include suppression of deadly antimicrobial free radicals such as nitric oxide (NO), faulty antigen presentation, selective induction and suppression of host cell apoptosis, inhibition of cytokine production and hence cytokine-inducible macrophage function, and activation of T cells (4-8). Leishmania has evolved sophisticated mechanisms to alter the physiological program and activation of adaptive immune responses of host cells by exploiting host cell signaling mechanisms such as the downregulation of Ca 2ϩ -dependent classical protein kinase C (PKC) activity and extracellular signal-regulated kinase (ERK) phosphorylation and activity (9, 10). Using mainly host tyrosine phosphatases, Leishmania is known to deactivate mitogen-activated protein kinases (MAPKs) in infected macrophages (5). Extensive manipulations of host cell effector (innate and adaptive) functions by pathogens must be reflected at the levels of transcripts as well as proteins. Enormous efforts made in the field of host gene expression profiling using different (murine and/or human) cell types and different species of Leishmania provide key insights into an extensive modulation of gene function and contribute to a better understanding of the dynamics of gene expres-

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“…As in the articles about Trypanosoma experiments, no article about Leishmania , Toxoplasma and Plasmodium experiments met all criteria that should be reported on our checklist, although one publication on Leishmania [49] scored 100% for the parasite and host domains (Table S5 and S6). There was no significant difference in the percentage of reported information between Trypanosoma , Leishmania , Toxoplasma and Plasmodium experiments (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

The Quality of Methods Reporting in Parasitology Experiments

et al. 2014

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There is a growing concern both inside and outside the scientific community over the lack of reproducibility of experiments. The depth and detail of reported methods are critical to the reproducibility of findings, but also for making it possible to compare and integrate data from different studies. In this study, we evaluated in detail the methods reporting in a comprehensive set of trypanosomiasis experiments that should enable valid reproduction, integration and comparison of research findings. We evaluated a subset of other parasitic (Leishmania, Toxoplasma, Plasmodium, Trichuris and Schistosoma) and non-parasitic (Mycobacterium) experimental infections in order to compare the quality of method reporting more generally. A systematic review using PubMed (2000–2012) of all publications describing gene expression in cells and animals infected with Trypanosoma spp was undertaken based on PRISMA guidelines; 23 papers were identified and included. We defined a checklist of essential parameters that should be reported and have scored the number of those parameters that are reported for each publication. Bibliometric parameters (impact factor, citations and h-index) were used to look for association between Journal and Author status and the quality of method reporting. Trichuriasis experiments achieved the highest scores and included the only paper to score 100% in all criteria. The mean of scores achieved by Trypanosoma articles through the checklist was 65.5% (range 32–90%). Bibliometric parameters were not correlated with the quality of method reporting (Spearman's rank correlation coefficient <−0.5; p>0.05). Our results indicate that the quality of methods reporting in experimental parasitology is a cause for concern and it has not improved over time, despite there being evidence that most of the assessed parameters do influence the results. We propose that our set of parameters be used as guidelines to improve the quality of the reporting of experimental infection models as a pre-requisite for integrating and comparing sets of data.

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Simultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE

Cited by 57 publications

References 50 publications

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Proteomic-Based Approach To Gain Insight into Reprogramming of THP-1 Cells Exposed to Leishmania donovani over an Early Temporal Window

The Quality of Methods Reporting in Parasitology Experiments

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