On 26 March 2008, simultaneous measurements of a large substorm were made using the Poker Flat Incoherent Scatter Radar, Time History of Events and Macroscale Interactions during Substorm (THEMIS) spacecraft, and all sky cameras. After the onset, electron precipitation reached energies ≳100 keV leading to intense D region ionization. Identifying the source of energetic precipitation has been a challenge because of lack of quantitative and magnetically conjugate measurements of loss cone electrons. In this study, we use the maximum entropy inversion technique to invert altitude profiles of ionization measured by the radar to estimate the loss cone energy spectra of primary electrons. By comparing them with magnetically conjugate measurements from THEMIS‐D spacecraft in the nightside plasma sheet, we constrain the source location and acceleration mechanism of precipitating electrons of different energy ranges. Our analysis suggests that the observed electrons ≳100 keV are a result of pitch angle scattering of electrons originating from or tailward of the inner plasma sheet at ~9RE, possibly through interaction with electromagnetic ion cyclotron waves. The electrons of energy 10–100 keV are produced by pitch angle scattering due to a potential drop of ≲10 kV in the auroral acceleration region (AAR) as well as wave–particle interactions in and tailward of the AAR. This work demonstrates the utility of magnetically conjugate ground‐ and space‐based measurements in constraining the source of energetic electron precipitation. Unlike in situ spacecraft measurements, ground‐based incoherent scatter radars combined with an appropriate inversion technique can be used to provide remote and continuous‐time estimates of loss cone electrons in the plasma sheet.