Simultaneous HPLC-DAD analysis of five flavonoids in diabetic rat plasma and its application in the study of pharmacokinetics

Abstract

The retraction of the above article has been agreed due to the overlap of the method validation data reproduced in this article with that

Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application ofGinkgo bilobaextracts on the pharmacokinetic

“…Due to the significant difference in polarity and concentration for the selected nine flavonoids, simultaneous determination of them in plasma becomes difficult in a single HPLC run. Although there are literatures [17,[19][20][21][22] concerning the determination of rutin, quercitrin, quercetin, kaempferol, and isorhamnetin simultaneously in G. biloba extract solid oral dosage or plasma using HPLC with relatively fine sensitivity, the methods may not be appropriate for the study of GBE in vivo for low drug concentration and interference of endogenous substance in plasma. Our laboratory has published simultaneous determination of five flavonoids in GBE in rat plasma [17]; however, the chromatographic condition is not suitable for the simultaneous separation, and determination of selected nine flavonoids of GBE in beagle dog plasma and the plasma was two-step prepared with acetone-ether (1:14) and methanol, respectively.…”

“…Referring to our previous research [17], kromasil C 18 column and methanol-0.2% formic acid were selected as stationary and mobile phase, respectively. In present study, optimization of mobile phase was focused.…”

“…However, method has not previously been described for the simultaneous determination of rutin, myricetin, quercitrin, quercetin, luteolin, genistein, kaempferol, apigenin, and isorhamnetin in vivo in literature. Our laboratory has reported the simultaneous determination of five flavonoids (rutin, quercetrin, quercetin, kaempferol, and isorhamnetin) in rat plasma after intravenous administration of GBE [17], but the method cannot be used in the simultaneous determination of selected nine flavonoids in GBE in plasma, and plasma sample preparation is cumbersome.…”

Zwitterionic Cocrystals of Flavonoids and Proline: Solid-State Characterization, Pharmaceutical Properties, and Pharmacokinetic Performance

“…To accurately quantify the concentration of Kae in the plasma, a modified method based on previously reported work was employed using high performance liquid chromatography. , In brief, frozen plasma samples were thawed at room temperature before sample preparation. 100 μL of rat plasma was mixed with 15 μL of genistein solution (internal standard, 20.33 μg/mL in metanol) and 50 μL of 20% phosphoric acid solution.. After vortexing for 2 min, the plasma samples were extracted with 200 μL acetonitrile by vortex-mixing for 5 min and centrifuged for 10 min (10 °C, 10 000 g ).…”

Intravenous rutin in rat exacerbates isoprenaline-induced cardiotoxicity likely due to intracellular oxidative stress

“…A.1) suggests that the positive effects of oral rutin on the ISO-induced oxidative stress were caused by its metabolites, including quercetin conjugates and small phenolic acids, and/or some adaptation mechanism(s). [53][54][55] Therefore, if rutin had positive effects, these benefits probably were not caused by rutin per se.…”