Simultaneous identification by multiplex PCR of major <i>Prototheca</i>
 spp. isolated from bovine and buffalo intramammary infection and bulk tank

Capra, Emanuele; Cremonesi, Paola; Cortimiglia, Claudia; Bignoli, Giulia; Ricchi, M.; Moroni, P.; Pesce, A.; Luini, M.; Castiglioni, Bianca

doi:10.1111/lam.12326

Cited by 22 publications

(22 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Culture dependent approaches demonstrated that most frequently isolated bacteria during mastitis are coagulase negative, causing 78% of intramammary infections cases of mastitis [ 31 , 32 ], Prototeca spp. and Streptococcus pluranimalium being found occasionally [ 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

The microbiota of water buffalo milk during mastitis

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to define the microbiota of water buffalo milk during sub-clinical and clinical mastitis, as compared to healthy status, by using high-throughput sequencing of the 16S rRNA gene. A total of 137 quarter samples were included in the experimental design: 27 samples derived from healthy, culture negative quarters, with a Somatic Cell Count (SCC) of less than 200,000 cells/ml; 27 samples from quarters with clinical mastitis; 83 samples were collected from quarters with subclinical mastitis, with a SCC number greater of 200,000 cells/ml and/or culture positive for udder pathogens, without clinical signs of mastitis. Bacterial DNA was purified and the 16S rRNA genes were individually amplified and sequenced. Significant differences were found in milk samples from healthy quarters and those with sub-clinical and clinical mastitis. The microbiota diversity of milk from healthy quarters was richer as compared to samples with sub-clinical mastitis, whose microbiota diversity was in turn richer as compared to those from clinical mastitis. The core microbiota of water buffalo milk, defined as the asset of microorganisms shared by all healthy milk samples, includes 15 genera, namely Micrococcus, Propionibacterium, 5-7N15, Solibacillus, Staphylococcus, Aerococcus, Facklamia, Trichococcus, Turicibacter, 02d06, SMB53, Clostridium, Acinetobacter, Psychrobacter and Pseudomonas. Only two genera (Acinetobacter and Pseudomonas) were present in all the samples from sub-clinical mastitis, and no genus was shared across all in clinical mastitis milk samples. The presence of mastitis was found to be related to the change in the relative abundance of genera, such as Psychrobacter, whose relative abundance decreased from 16.26% in the milk samples from healthy quarters to 3.2% in clinical mastitis. Other genera, such as SMB53 and Solibacillus, were decreased as well. Discriminant analysis presents the evidence that the microbial community of healthy and clinical mastitis could be discriminated on the background of their microbiota profiles.

show abstract

Section: Introductionmentioning

confidence: 99%

The microbiota of water buffalo milk during mastitis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In summary, pathogenesis of P. bovis, a globally ubiquitous and environmental (grass, water, trees) algae (Rösler et al, 2003) capable of infecting various vertebrate hosts (Seok et al, 2013;Capra et al, 2014;Carfora et al, 2017) remains poorly understood. Therapeutic control for human and animal protothecosis is on demand.…”

Section: Discussionmentioning

confidence: 99%

Murine and Human Cathelicidins Contribute Differently to Hallmarks of Mastitis Induced by Pathogenic Prototheca bovis Algae

Shahid

Cavalcante

Knight

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Prototheca bovis (formerly P. zopfii genotype-II) is an opportunistic, achlorophyllous alga that causes mastitis in cows and skin disease in cats and dogs, as well as cutaneous lesions in both immunocompetent and immunosuppressed humans. Antifungal medications are commonly ineffective. This study aimed to investigate innate immune responses contributed by cathelicidins to P. bovis in the mammary gland using a mastitis model in mice deficient in the sole murine cathelicidin (Camp). We determined P. bovis caused acute mastitis in mice and induced Camp gene transcription. Whereas, Camp −/− and Camp +/+ littermates had similar local algae burden, Camp +/+ mice produced more pro-inflammatory cytokines, TNF-α, and Cxcl-1. Likewise, Camp +/+ bone marrow-derived macrophages were more responsive to P. bovis, producing more TNF-α and Cxcl-1. Human cathelicidin (LL-37) exhibited a different effect against P. bovis; it had direct algicidal activity against P. bovis and lowered TNF-α, Cxcl-1, and IL-1β production in both cultured murine macrophages and mammary epithelial cells exposed to the pathogenic algae. In conclusion, cathelicidins were involved in protothecosis pathogenesis, with unique roles among the diverse peptide family. Whereas, endogenous cathelicidin (Camp) was key in mammary gland innate defense against P. bovis, human LL-37 had algicidal and immunomodulatory functions.

show abstract

“…In the last few decades, comprehensive studies focusing on the genotyping of Prototheca species have enabled advancements in the epidemiology of protothecosis among domestic animals, particularly in relation to the geographical distribution of these microalgae (Roesler et al 2006;Ahrholdt et al 2012;Morandi et al 2016). Similar investigations have also reported P. zopfii genotype 2 as the main pathogenic type involved in bovine mastitis globally (M€ oller et al 2007;Jagielski et al 2011;Ahrholdt et al 2012;Capra et al 2014;Morandi et al 2016).…”

Section: Genotypic Characterization Of P Zopfiimentioning

confidence: 93%

In vitro algicidal effect of guanidine on Prototheca zopfii genotype 2 strains isolated from clinical and subclinical bovine mastitis

Alves

Capra

Morandi

et al. 2017

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

Prototheca zopfii genotype 2 is an opportunistic pathogen of bovine mastitis. To date, no effective therapies against protothecal mastitis have been developed. The in vitro algicidal effect of guanidine on 75 P. zopfii genotype 2 strains isolated from cows revealed that all of the isolates were susceptible to the compound at low concentrations, which indicates that guanidine may be used as an antiseptic/disinfectant for dairy milking equipment, in pre- and postdipping solutions, and as a chemical dry therapy or an intramammary therapy. This study describes the in vitro algicidal effect of guanidine on P. zopfii for the first time.

show abstract

Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank

Abstract: This work reports PCR assays based on novel Prototheca spp. mitochondrial and chloroplastic target sequences. The multiplex PCR protocol described in this study is useful for rapid simultaneous detection of P. zopfii, P. wickerhamii and P. blaschkeae.

Cited by 22 publications

References 27 publications

The microbiota of water buffalo milk during mastitis

The microbiota of water buffalo milk during mastitis

Murine and Human Cathelicidins Contribute Differently to Hallmarks of Mastitis Induced by Pathogenic Prototheca bovis Algae

In vitro algicidal effect of guanidine on Prototheca zopfii genotype 2 strains isolated from clinical and subclinical bovine mastitis

Contact Info

Product

Resources

About