Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor in pseudophakic glaucomatous eyes

Inoue, Toshihiro; Kawaji, Takahiro; Inatani, Masaru; Kameda, Takuro; Yoshimura, Nagahisa; Tanihara, Hidenobu

doi:10.1016/j.jcrs.2012.04.028

Cited by 99 publications

(68 citation statements)

References 36 publications

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“…9,15,[20][21][22][23] To assess the influence of previous ocular surgeries on the cytokine levels in AqH, we conducted a correlation analysis between the two (Table 3). The number of previous ocular surgeries was strongly positively correlated with the following cytokine levels: IL-1a (r ¼ 0.47, P < 0.0001), IL-4 (r ¼ 0.46, P < 0.0001), IL-6 (r ¼ 0.50, P < 0.0001), IL-8 (r ¼ 0.50, P < 0.0001), IL-10 (r ¼ 0.48, P < 0.0001), IL-13 (r ¼ 0.38, P < 0.0001), IL-17A (r ¼ 0.32, P < 0.0001), MIP-1b (r ¼ 0.42, P < 0.0001), sICAM-1 (r ¼ 0.42, P < 0.0001), MCP-1 (r ¼ 0.26, P ¼ 0.001), P-selectin (r ¼ 0.26, P ¼ 0.001), and E-selectin (r ¼ 0.29, P ¼ 0.0002).…”

Section: Correlations Between Cytokine Levels In Aqh and The Number Omentioning

confidence: 99%

“…We confirmed the significant correlation between some cytokine levels in AqH and the number of previous surgeries, which is in line with previous studies. 20,22,23 However, although the number of the previous surgeries was small (0.5 6 0.5, ranging from 0 to 1) in eyes with a low ECD, the aqueous levels of IL-1a, IL-8, IL-17A, TNFa, GM-CSF, MIP-1a, IFN-c, and E-selectin were significantly elevated in eyes with a low ECD, compared with healthy controls, suggesting that ocular surgeries were not the solitary factor responsible for the elevation of cytokine levels. Further studies using multivariate analyses are necessary to assess clinical factors associated with elevated levels of cytokines and decreases in ECD.…”

Section: Figurementioning

confidence: 99%

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Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density

Yamaguchi

Higa

Suzuki

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Section: Correlations Between Cytokine Levels In Aqh and The Number Omentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density

Yamaguchi

Higa

Suzuki

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Anatomically, the aqueous humor (AqH) is present between the corneal endothelium and the iris, and it has been reported that inflammatory cytokines in the AqH increase during various pathological processes. [15][16][17][18] In an in vitro study, a combination of proinflammatory cytokines synergistically induced the apoptosis of corneal endothelial cells. 19 We recently showed that inflammatory cytokine levels were elevated in the AqH of eyes with bullous keratopathy and reduced ECDs, 20 and that iris damage was associated with an elevation in aqueous cytokine levels.…”

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confidence: 99%

Preoperative Aqueous Cytokine Levels are Associated With Endothelial Cell Loss After Descemet's Stripping Automated Endothelial Keratoplasty

Yazu

Yamaguchi

Aketa

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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Citation: Yazu H, Yamaguchi T, Aketa N, et al. Preoperative aqueous cytokine levels are associated with endothelial cell loss after Descemet's stripping automated endothelial keratoplasty. Invest Ophthalmol Vis Sci. 2018;59:612-620. https://doi.org/ 10.1167/iovs.17-23049 PURPOSE. To evaluate the association between endothelial cell density (ECD) after Descemet's stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH).METHODS. This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) or cataract surgery (33 eyes). AqH samples were collected at the beginning of each surgery. The levels of cytokines (IL-1a, IL-1b, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-a, IFN-c, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule [sICAM]-1) in the AqH were measured by multiplex beads immunoassay. The correlations between preoperative aqueous cytokine levels and the ECD at 12 months after DSAEK were analyzed.RESULTS. The ECD decreased from 2747 6 259 cells/mm 2 in the donor graft to 1235 6 607 cells/mm 2 at 12 months after DSAEK. In all subjects undergoing DSAEK, the postoperative ECD at 12 months was significantly correlated with the preoperative levels of MCP-1 (r ¼ À0.467, 95% confidence interval [CI]: À0.650 to À0.222, P ¼ 0.0003). In an analysis excluding Fuchs endothelial corneal dystrophy (11 eyes), the ECD at 12 months after DSAEK was significantly correlated with preoperative levels of IL-17A (r ¼ À0.635, 95% CI: À0.819 to À0.319, P ¼ 0.0004), MCP-1 (r ¼ À0.605, 95% CI: À0.779 to À0.345, P < 0.0001), IFN-c (r ¼ À0.633, 95% CI: À0.796 to À0.385, P < 0.0001), E-selectin (r ¼ À0.516, 95% CI: À0.756 to À0.276, P ¼ 0.0004), and sICAM-1 (r ¼ À0.537, 95% CI: À0.735 to À0.253, P ¼ 0.0005).CONCLUSIONS. Higher preoperative levels of IL-17A, MCP-1, IFN-c, E-selectin, and sICAM-1 in the AqH were associated with lower ECD after DSAEK for bullous keratopathy.

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“…6 In our previous studies using aqueous humor samples obtained from the patients with glaucoma, elevated levels of MCP-1 were found in pseudophakic eyes after cataract surgery. 8 In addition, a previous study evaluating aqueous MCP-1 levels in the same patients before and after phacoemulsification also demonstrated increased MCP-1 levels postoperatively in cataract eyes (nonglaucomatous eyes; Kawai M, et al IOVS 2012;53:ARVO EAbstract 2503). Interestingly, the postsurgical percentage change from the baseline aqueous MCP-1 level was negatively correlated with the baseline of IOP in the study.…”

Section: Discussionmentioning

confidence: 89%

“…On the other hand, our previous study showed that aqueous humor obtained from eyes with open-angle glaucoma contained increased monocyte chemoattractant protein-1 (MCP-1)/CC chemokine ligand 2 and IL-8 levels after phacoemulsification. 8 In another study, we also observed elevated MCP-1 and IL-8 levels in the aqueous humor obtained from non-glaucomatous eyes after phacoemulsification compared with that before surgery (Kawai M, et al IOVS 2012;53:ARVO E-Abstract 2503). Regarding the effects of IL-8 on the conventional outflow route, IL-8 decreases the resistance of the Schlemm's canal epithelial (SCE) cell sheet.…”

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confidence: 81%

The Effect of Monocyte Chemoattractant Protein-1/CC Chemokine Ligand 2 on Aqueous Humor Outflow Facility

Tsuboi

Inoue

Kawai

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the effect of monocyte chemoattractant protein-1 (MCP-1)/CC chemokine ligand 2 on aqueous humor outflow facility.METHODS. Aqueous humor outflow facility was measured in enucleated porcine eyes in a constant pressure perfusion system with or without MCP-1 (1600 ng/mL). Expression of CCR2, an MCP-1 receptor, in Schlemm's canal endothelial (SCE) cells was examined by reverse transcription-polymerase chain reaction (RT-PCR) assay. The effect of MCP-1 (0-1600 ng/ mL) on SCE cell viability was evaluated using a WST-8 assay.The effect of MCP-1 (0-800 ng/mL) on SCE-cell monolayer permeability was evaluated with or without a CCR2 antagonist (10 nM) by measuring transendothelial electrical resistance (TEER). The intracellular localization of the gap junction protein ZO-1 was analyzed by immunofluorescence staining of SCE cells. RESULTS.The aqueous humor outflow facility increased significantly from basal levels at 80 minutes after perfusion with MCP-1 compared with control eyes (21.2% 6 6.6% [MCP-1] vs. 5.7 6 2.5% [control]; P ¼ 0.048). CCR2 was detected by RT-PCR. Cell viability was not affected by MCP-1 treatment. TEER of SCE-cell monolayer at 3 hours after treatment with 800 ng/mL MCP-1 decreased by 21.6 6 1.7% compared with controls (P ¼ 0.014), and the TEER-decreasing effects of MCP-1 were attenuated by a CCR2 antagonist. Immunocytochemical staining revealed a modest disruption of ZO-1 in MCP-1-treated SCE cells. CONCLUSIONS.The present results revealed that MCP-1 increased aqueous humor outflow facility and decreased TEER via CCR2. These findings suggest that MCP-1 modulates aqueous humor outflow through the conventional pathway. (Invest Ophthalmol Vis Sci. 2012;53:6702-6707) DOI:10.1167/iovs.12-10376 C ataract surgery affects postoperative intraocular pressure (IOP). Although phacoemulsification has become the standard choice for surgical treatment of cataracts using minimally invasive procedures, the effects on IOP reduction after classical large incision cataract surgery are clinically similar.1 The reduction in IOP after cataract surgery is positively related to preoperative IOP, and inversely related to preoperative anterior chamber depth.2,3 Interestingly, IOP after phacoemulsification is decreased more in glaucomatous eyes compared with non-glaucomatous eyes, and remains reduced for a long period. 4 The exact mechanisms related to the IOP-reducing effects of phacoemulsification, however, are not yet thoroughly understood.Interleukin (IL)-1a increases outflow facility. 5,6 In addition, in cultured trabecular meshwork (TM) cells, phacoemulsification ultrasound upregulates interleukin IL-1a and endothelial leukocyte adhesion molecule-1, 7 suggesting potential effects of IL-1a on IOP reduction after phacoemulsification. On the other hand, our previous study showed that aqueous humor obtained from eyes with open-angle glaucoma contained increased monocyte chemoattractant protein-1 (MCP-1)/CC chemokine ligand 2 and IL-8 levels after phacoemulsification. 8 In another study, we also observed el...

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Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor in pseudophakic glaucomatous eyes

Abstract: No author has a financial or proprietary interest in any material or method mentioned.

Cited by 99 publications

References 36 publications

Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density

Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density

Preoperative Aqueous Cytokine Levels are Associated With Endothelial Cell Loss After Descemet's Stripping Automated Endothelial Keratoplasty

The Effect of Monocyte Chemoattractant Protein-1/CC Chemokine Ligand 2 on Aqueous Humor Outflow Facility

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