Simultaneous isolation and purification of cucurbitacin D and I from Ecballium elaterium (l.) A. Rich fruit juice

Tosun, Emir; Baysar, Ahmet

doi:10.20450/mjcce.2019.1648

Cited by 5 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compound 8 was isolated as a solid melting at 150–151 °C [lit. 151.2 °C] . Its TLC showed a spot at R f 036 with petroleum ether/EtOAc (6:4) was used as a mobile phase.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

In Silico Molecular Docking Analysis, Cytotoxicity, and Antibacterial Activities of Constituents of Fruits of Cucumis dipsaceus

Assefa,

Tesso,

Ramachandran

et al. 2023

ACS Omega

View full text Add to dashboard Cite

Cucumis dipsaceus (Cucurbitaceae) is a plant traditionally used against diarrhea, teeth-ach, wounds, stomach ache, meningitis, and cancer. The extracts of C. dipsaceus after silica gel column chromatography gave nine compounds identified using spectroscopic methods such as hexacosane (1), octadecane (2), 17-(-5-ethyl-2,6-dihydroxy-6-methylhept-3-en-2-yl)-9-(hydroxymethyl)-13-methylcyclopenta[α]phenanthren-3-ol (3), erythrodiol (4), (9,12)-propyl icosa-9,12-dienoate (5), α-spinasterol (6), 16-dehydroxycucurbitacin (7), cucurbitacin D (8), and 23,24-dihydroisocucurbitacin D (9). Compounds 3 and 4 are new to the genus Cucumis. α-Spinasterol showed better inhibition zone diameter = 13.67 ± 0.57, 15.00 ± 0.10, and 13.33 ± 0.57 mm against Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes compared with the other tested samples. α-Spinasterol (−8.0 kcal/mol) and 3 (−7.6 kcal/mol) displayed high binding affinity against DNA Gyrase compared to ciprofloxacin (−7.3 kcal/mol). α-Spinasterol and 16-dehydroxycucurbitacin showed better binding affinity against protein kinase. The cytotoxicity results revealed that the EtOAc extract showed the highest potency with IC50 = 16.05 μg/mL. 16-Dehydroxycucurbitacin showed a higher binding affinity (−7.7 kcal/mol) against human topoisomerase IIβ than etoposide. The cytotoxicity and antibacterial activities and in silico molecular docking analysis displayed by the constituents corroborate the traditional use of the plant against bacteria and cancer.

show abstract

“…Compound 8 was isolated as a solid melting at 150–151 °C [lit. 151.2 °C] . Its TLC showed a spot at R f 036 with petroleum ether/EtOAc (6:4) was used as a mobile phase.…”

Section: Resultsmentioning

confidence: 99%

“…151.2 °C]. 36 Its TLC showed a spot at R f 036 with petroleum ether/EtOAc (6:4) was used as a mobile phase. Based on 1 H, 13 C NMR, and DEPT-135 spectral data and comparison with literature value, compound 8 is in good agreement with cucurbitacin D (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

In Silico Molecular Docking Analysis, Cytotoxicity, and Antibacterial Activities of Constituents of Fruits of Cucumis dipsaceus

Assefa,

Tesso,

Ramachandran

et al. 2023

ACS Omega

View full text Add to dashboard Cite

show abstract

“…CuI was prepared as described by Tosun and Baysar Experimental studies were generally carried out (Tosun & Baysar, 2019) in 3 stages. The Ecballium elaterium plant, which grows in Osmaniye, Turkey, was first collected during the season.…”

Section: Methodsmentioning

confidence: 99%

“…CuI was prepared as described by Tosun and Baysar Experimental studies were generally carried out (Tosun & Baysar, 2019) in 3 stages.…”

Section: Preparation and Isolation Of Extract Of Plantsmentioning

confidence: 99%

“…Although E. elaterium contains many Cucurbitacin subspecies, generally, it is cucurbitacin B, D, I, and E that are isolated from the juice of this plant (Hu et al, 2017). Plant collection, extraction, and purification processes for obtaining cucurbitacins were conducted following the method previously described by Tosun and Baysar (Tosun & Baysar, 2019). The fruit juice of the E. elaterium plant was first extracted with hexane; wax-like substances were removed from the fruit juice and then extracted with chloroform to allow the organic phase to pass into the solvent.…”

Section: Isolation and Purification Of Cuimentioning

confidence: 99%

See 1 more Smart Citation

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Üremiş

Uremis

Çiğremiş

et al. 2022

Journal of Food Biochemistry

Self Cite

View full text Add to dashboard Cite

Hepatocellular carcinoma is a common cancer type, especially among men. Although cucurbitacin I (CuI), widely found in plants belonging to the Ecballium elaterium (E. L) plant family, has been shown to have antitumorigenic properties in many cancer types, its anticancer effect, molecular mechanism, and apoptotic effect mediated by signal pathways on hepatocellular carcinoma have not been fully clarified. In the present study, we investigated the anticancer effect of CuI treated at different doses on the HepG2 cell line and the underlying mechanism in vitro. High-purity CuI was obtained from the E. elaterium plant with the aid of HPLC. The effects of this substance on the viability of cells were studied by the MTT assay. The effects of CuI on cell cycle progression and apoptosis were studied with flow cytometry. DNA breaks were analyzed by the Comet assay method.The proteins and genes involved in the JAK/STAT3, MAPK/ERK, and AKT/mTOR signaling pathways were investigated using Western blot and qRT-PCR, respectively. The results of this study demonstrated that CuI significantly reduced HepG2 cell growth in vitro, induced antiproliferation, and G2/M phase of the cell cycle was interrupted. Practical applicationsCuI administration was shown to downregulate the levels of proteins in the PI3K/ AKT/mTOR, MAPK, and JAK2/STAT3 cascades in HepG2 cells. CuI also reduced the expression of MAPK, STAT3, mTOR, JAK2, and Akt genes in different concentrations.DNA breaks are formed as a result of this effect. CuI, by reducing cell proliferation and promoting apoptosis, was found to have potential as a chemotherapeutic agent of hepatocellular carcinoma.

show abstract

Cucurbitacin D Inhibits the Proliferation of HepG2 Cells and Induces Apoptosis by Modulating JAK/STAT3, PI3K/Akt/mTOR and MAPK Signaling Pathways

Uremis

Üremiş

Tosun

et al. 2022

CCDT

View full text Add to dashboard Cite

Background: Cucurbitacin D (CuD) is a natural compound that can be isolated in various plant families, mainly from Ecballium Elaterium (L.) A. Rich. (E. Elaterium). It is a triterpenoid with a broad spectrum of biological activity, including anti-cancer properties. Hepatocellular carcinoma, the aggressive type of liver cancer, is an important public health problem worldwide. Objective: In the present study, we investigated the anticancer effect of CuD treated at different doses on the HepG2 cell line and the underlying mechanism in vitro. Method: CuD was isolated from the fruit juice of E. Elaterium plant and quantitative analysis was performed using high-performance liquid chromatography. The cell viability effect of purified CuD was determined by the MTT test also cell apoptosis and cell cycle arrest effects were determined by flow cytometry. DNA damage was evaluated with the comet test. Proteins and genes involved in PI3K/AKT/mTOR, MAPK, and JAK2/STAT3 signaling pathways were evaluated by western blot and qRT-PCR. Result: CuD showed both antiproliferative and cytotoxic effects against the HepG2 cell line in a dose and time-dependent manner. It was observed that CuD induced apoptosis and blocked the cell cycle in HepG2 cells. It was observed that the expression of genes and some proteins that play a key role in PI3K/AKT/mTOR, MAPK, and JAK2/STAT3 cascades were dose-dependently down-regulated and activated the apoptotic pathway. Conclusion: All these results; show promise that CuD may have a therapeutic effect in liver cancer with HCC.

show abstract

Simultaneous isolation and purification of cucurbitacin D and I from Ecballium elaterium (l.) A. Rich fruit juice

Cited by 5 publications

References 27 publications

In Silico Molecular Docking Analysis, Cytotoxicity, and Antibacterial Activities of Constituents of Fruits of Cucumis dipsaceus

In Silico Molecular Docking Analysis, Cytotoxicity, and Antibacterial Activities of Constituents of Fruits of Cucumis dipsaceus

Cucurbitacin I exhibits anticancer efficacy through induction of apoptosis and modulation of JAK / STAT3 , MAPK / ERK , and AKT / mTOR signaling pathways in HepG2 cell line

Cucurbitacin D Inhibits the Proliferation of HepG2 Cells and Induces Apoptosis by Modulating JAK/STAT3, PI3K/Akt/mTOR and MAPK Signaling Pathways

Contact Info

Product

Resources

About