Simultaneous Liquid Chromatographic Determination of Thiabendazole, o-Phenylphenol, and Diphenyl Residues in Citrus Fruits, Without Prior Cleanup

Abstract

A simple, rapid, efficient method has been developed for determining thiabendazole, o-phenylphenol, and diphenyl in citrus fruits by using high performance liquid chromatography, with fluorescence or ultraviolet detection. The compounds are extracted with ethyl acetate and separated from soluble fruit components on a LiChrosorb RP-8 column. Recovery of these compounds added to citrus fruits at 5 or 50 ppm levels was >93%; the limit of detection for the compounds is 1 ppm.

Spectrofluorometric determination of pesticide residue mixtures by isodifferential derivative spectroscopy

“…The results are obtained as mean values of triplicate analysis. As previously reported by Kitada et al (11), ethyl acetate gives the best efficiency for extracting TBZ, MBC, and related compounds and it was used in the present work.…”

“…Several papers have been published regarding analysis of thiabendazole (TBZ), MBC, or analogues. Most of the procedures involve rigorous cleanup procedures of sample extracts, and the final measurement is usually by spectrofluorometry (4,5), spectrophotometry (6,7), and high-performance liquid chromatography (HPLC) (8)(9)(10)(11).…”