Simultaneous liquid chromatography–fluorescence analysis of type A and type B trichothecenes as fluorescent derivatives via reaction with coumarin-3-carbonyl chloride

Dall’Asta, Chiara; Galaverna, Gianni; Biancardi, Alberto; Gasparini, Mara; Sforza, Stefano; Dossena, Arnaldo; Marchelli, Rosangela

doi:10.1016/j.chroma.2004.07.002

Cited by 37 publications

(20 citation statements)

References 22 publications

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“…Various chromatographic methods commonly used for quantitative determination of trichothecenes in cereal-based products include gas chromatography with electron capture, flame ionisation or mass spectrometry (MS) detectors (Nielsen and Thrane 2001;Schothorst and Jekel 2001;Ibáñez-Vea et al 2011;Valle-Algarra et al 2011) and highperformance liquid chromatography (HPLC) with ultraviolet, diode array, fluorescence or MS detectors (Jiménez et al 2000;Dall'Asta et al 2004;Biancardi et al 2005;Santini et al 2009;Monaci et al 2011). Liquid chromatography (LC)-MS spectrometers with triple quadrupole mass analysers have been most frequently used in labs dealing with trichothecene analyses.…”

Section: Introductionmentioning

confidence: 99%

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

et al. 2013

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The aim of this work was to evaluate the usefulness of the ion trap mass spectrometry coupled to high-performance liquid chromatography for simultaneous determination of selected trichothecenes (nivalenol, deoxynivalenol, fusarenon-X, neosolaniol, 3-acetyl-deoxynivalenol, diacetoxyscirpenol, HT-2 and T-2 toxins) in grain products. These compounds were extracted from the grain products and then cleaned up with the developed, simple and robust procedure using some mixture of neutral alumina, charcoal and diatomaceous earth. Method recovery was 88-125 % depending on combination of the analysed mycotoxins, sample matrix and the fortification level. Method precision expressed by relative standard deviation ranged from 2.6 to 27.4 %. The concentrations of the selected trichothecenes have been determined in 94 samples of cerealbased products. Maize-based next to wheat-based products were the most contaminated with deoxynivalenol, neosolaniol, 3-acetyl-deoxynivalenol, diacetoxyscirpenol and HT-2 toxin. In 83 % of wheat-based products, deoxynivalenol was determined at the average level of 249 μg kg −1 . The highest concentration of deoxynivalenol-2,026 μg kg −1 (476±471 μg kg −1 on the average)-was found in the maize-based product. Other mycotoxins were found much less frequently: 3-acetyl-deoxynivalenol in only one sample at the concentration of 59 μg kg −1 , neosolaniol, HT-2 toxin and diacetoxyscirpenol in a few samples on average concentrations close to respective limits of quantification.

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Section: Introductionmentioning

confidence: 99%

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

et al. 2013

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“…Coumarin that is commonly isolated from the tonka beans has a sweet odour and is widely used in soaps and cosmetics (Lake, 1999). It can be found in several foods, in particular in cereals (e.g., wheat, maize, barley, and oat) and several derived products, such as corn flakes, flour, infant foods, malt, and beer (Dall'Asta et al, 2004). Coumarin glycosides have been shown to have blood-thinning, anti-fungicidal, and anti-tumour activities.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of diethylene glycol, diethylene glycol monoethyl ether, coumarin and caffeine in food items by gas chromatography

et al. 2011

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“…Techniques to detect type A trichothecene structures have included chromatographic technologies such as gas chromatography (GC) (Valle-Algarra, Mateo, Mateo, Gimeno-Adelantado, & Jimenez, 2011), gas chromatography-mass spectrometry (GC-MS) (Eskola & Rizzo, 2001), gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) (Jekel & Van Egmond, 2014;Nielsen & Thrane, 2000;Rodríguez-Carrasco, Berrada, Font, & Manes, 2012), liquid chromatography-mass spectrometry (LC-MS) (Dall'Astaa et al, 2004;Fuchs, Rabus, Handl, & Binder, 2003;Nakagawa, Sakamoto, Sago, Kushiro, & Nagashima, 2013;Razzazi-Fazeli, Rabus, Cecon, & Bohm, 2002) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Tamura, Nakagawa, Uyama, & Mochizuki, 2014;Yang et al, 2013). Although these detection technologies characteristically have a high-flux and low detection limit, not all type A trichothecenes and their metabolites are recognized especially if the concentration of the parent compound is extremely low and the metabolites are unknown.…”

Section: Introductionmentioning

confidence: 99%

In vitrosynthesis of a type A trichothecenes complete antigen from T-2 toxin

Wang

et al. 2016

Food and Agricultural Immunology

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We report the artificial synthesis from T-2 toxin of a type A trichothecenes complete antigen. First, 3-AcT -2 was made from T-2 following acetylation. Then 3-Ac-NEOS as a T-2 skeleton structure was synthesized by enzymolysis and identified by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance. Next, a hapten (3-Ac-NEOS-HS) was formed by modifying the C8 position of 3-Ac-NEOS using succinic anhydride method. 3-Ac-NEOS-HS-BSA/-OVA were prepared by conjugating hapten with bovine serum albumin (BSA) or ovalbumin (OVA) by carbodiimide method and identified by UV spectroscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Results showed that conjugation ratio of 3-Ac-NEOS-HS to BSA was 8.76: 1 and OVA 7.24: 1, indicating 3-Ac-NEOS-HS-BSA as a complete antigen was better. Next we used it to immunize rabbits and obtained a 1:64,000 antibody titre. In conclusion, a type A trichothecenes complete antigen was successfully synthesized, which was the foundation for antibody preparation and enzyme-linked immunosorbent assay kit development for all type A trichothecenes (parent + modified/ masked type A trichothecenes).

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Simultaneous liquid chromatography–fluorescence analysis of type A and type B trichothecenes as fluorescent derivatives via reaction with coumarin-3-carbonyl chloride

Cited by 37 publications

References 22 publications

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

Simultaneous determination of diethylene glycol, diethylene glycol monoethyl ether, coumarin and caffeine in food items by gas chromatography

In vitrosynthesis of a type A trichothecenes complete antigen from T-2 toxin

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