Simultaneous measurement of dissolved oxygen concentration and velocity field in microfluidics using oxygen-sensitive particles

Kim, Hyun Dong; Yi, Seung Jae; Kim, Kyung Chun

doi:10.1007/s10404-012-1130-4

Cited by 14 publications

(9 citation statements)

References 18 publications

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“…Such dyes can be incorporated into the media and flowed through the devices, [64][65][66] patterned as spots or thin films that are integrated into the device at the time of fabrication, 29,[67][68][69] or as particles or beads that are introduced into the device after assembly. [70][71][72] In this study, we used a solution of RTDP in media in order to measure oxygen temporally and spatially throughout the LAMPS volume. Although RTDP concentrations higher than 0.2 mM were reported to be phototoxic, lower concentrations were found to be cell compatible.…”

Section: Measuring Oxygen Tension In Lampsmentioning

confidence: 99%

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

Lee-Montiel

George

Gough

et al. 2017

Exp Biol Med (Maywood)

118

168

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This article describes our next generation human Liver Acinus MicroPhysiology System (LAMPS). The key demonstration of this study was that Zone 1 and Zone 3 microenvironments can be established by controlling the oxygen tension in individual devices over the range of ca. 3 to 13%. The oxygen tension was computationally modeled using input on the microfluidic device dimensions, numbers of cells, oxygen consumption rates of hepatocytes, the diffusion coefficients of oxygen in different materials and the flow rate of media in the MicroPhysiology System (MPS). In addition, the oxygen tension was measured using a ratiometric imaging method with the oxygen sensitive dye, Tris(2,2'-bipyridyl) dichlororuthenium(II) hexahydrate (RTDP) and the oxygen insensitive dye, Alexa 488. The Zone 1 biased functions of oxidative phosphorylation, albumin and urea secretion and Zone 3 biased functions of glycolysis, α1AT secretion, Cyp2E1 expression and acetaminophen toxicity were demonstrated in the respective Zone 1 and Zone 3 MicroPhysiology System. Further improvements in the Liver Acinus MicroPhysiology System included improved performance of selected nonparenchymal cells, the inclusion of a porcine liver extracellular matrix to model the Space of Disse, as well as an improved media to support both hepatocytes and non-parenchymal cells. In its current form, the Liver Acinus MicroPhysiology System is most amenable to low to medium throughput, acute through chronic studies, including liver disease models, prioritizing compounds for preclinical studies, optimizing chemistry in structure activity relationship (SAR) projects, as well as in rising dose studies for initial dose ranging. Impact statement Oxygen zonation is a critical aspect of liver functions. A human microphysiology system is needed to investigate the impact of zonation on a wide range of liver functions that can be experimentally manipulated. Because oxygen zonation has such diverse physiological effects in the liver, we developed and present a method for computationally modeling and measuring oxygen that can easily be implemented in all MPS models. We have applied this method in a liver MPS in which we are then able to control oxygenation in separate devices and demonstrate that zonation-dependent hepatocyte functions in the MPS recapitulate what is known about in vivo liver physiology. We believe that this advance allows a deep experimental investigation on the role of zonation in liver metabolism and disease. In addition, modeling and measuring oxygen tension will be required as investigators migrate from PDMS to plastic and glass devices.

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Section: Measuring Oxygen Tension In Lampsmentioning

confidence: 99%

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

Lee-Montiel

George

Gough

et al. 2017

Exp Biol Med (Maywood)

118

168

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“…The containers contain three inlets for the input of oxygen and nitrogen gases, as well as an insert for the oxygen meter (Hanna Instruments 98193) which was used to monitor dissolved oxygen (DO). One gas outlet was fitted with the rubber lid for free flow of gases into the chamber …”

Section: Methodsmentioning

confidence: 99%

“…One gas outlet was fitted with the rubber lid for free flow of gases into the chamber. 37 For calibration of oxygen in a hydrogel sphere, the alginate solution was mixed with 10−20 μL of FNOPs and stirred for 5 min using a magnetic stirrer and then the hydrogels of size ∼750 μm were generated by using the technique as specified in section 2.1.2.2. The generated hydrogels were collected and pipetted evenly onto the well gasket filled with the alginate.…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 99%

Fluorescence-Based Nano-Oxygen Particles for Spatiometric Monitoring of Cell Physiological Conditions

Koduri

Goudar

Shao

et al. 2018

ACS Appl. Mater. Interfaces

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Closed-loop artificial pancreas systems have recently been proposed as a solution for treating stage I diabetes by reproducing the function of the pancreas. However, there are many unresolved issues associated with their development, including monitoring and controlling oxygen, immune responses, and the optimization of glucose, all of which need to be monitored and controlled to produce an efficient and viable artificial organ that can become integrated in the patient and maintain homeostasis. This research focused on monitoring the oxygen concentration, specifically achieving this kinetically as the oxygen gradient in an artificial pancreas made of alginate spheres containing islet cells. Functional nanoparticles (NPs) for measuring the oxygen gradient in different hydrogel cellular environments using fluorescence-based (F) microscopy were developed and tested. By the ester bond, a linker Pluronic F127 was conjugated with a carboxylic acid-modified polystyrene NP (510 nm). A hydrophilic/hydrophobic interaction between the commercially available oxygen-sensitive fluorophore and F127 results in fluorescence-based nano-oxygen particles (FNOPs). The in-house synthesized FNOP was calibrated inside electrosprayed alginate-filled hydrogels and demonstrated a good broad dynamic range (2.73-22.23) mg/L as well as a resolution of -0.01 mg/L with an accuracy of ±4%. The calibrated FNOP was utilized for continuous measuring of the oxygen concentration gradient for cell lines RIN-m5F/HeLa for more than 5 days in alginate hydrogel spheres in vitro.

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“…H. D. Kim et al [61] used a conventional microparticle image velocimetry method for performing simultaneous measurements of dissolved oxygen concentration (DOC) and velocity fields. DOC was determined by applying oxygen-sensitive particles, which were produced by the dispersion polymerization method.…”

Section: Combination Of Particle-based Oxygen Sensing With Flow Velocimetrymentioning

confidence: 99%

Sensor Micro and Nanoparticles for Microfluidic Application

Mazetyte‐Stasinskiene

Köhler

2020

Applied Sciences

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Micro and nanoparticles are not only understood as components of materials but as small functional units too. Particles can be designed for the primary transduction of physical and chemical signals and, therefore, become a valuable component in sensing systems. Due to their small size, they are particularly interesting for sensing in microfluidic systems, in microarray arrangements and in miniaturized biotechnological systems and microreactors, in general. Here, an overview of the recent development in the preparation of micro and nanoparticles for sensing purposes in microfluidics and application of particles in various microfluidic devices is presented. The concept of sensor particles is particularly useful for combining a direct contact between cells, biomolecules and media with a contactless optical readout. In addition to the construction and synthesis of micro and nanoparticles with transducer functions, examples of chemical and biological applications are reported.

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Simultaneous measurement of dissolved oxygen concentration and velocity field in microfluidics using oxygen-sensitive particles

Cited by 14 publications

References 18 publications

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

Fluorescence-Based Nano-Oxygen Particles for Spatiometric Monitoring of Cell Physiological Conditions

Sensor Micro and Nanoparticles for Microfluidic Application

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