Simultaneous measurement of imatinib, nilotinib and dasatinib in dried blood spot by ultra high performance liquid chromatography tandem mass spectrometry

Determination of nilotinib in spiked plasma, urine, and capsules by high-performance liquid chromatography with fluorimetric detection

“…The retention time in the proposed method was considerably shorter than the published HPLC-UV methods [4,6,9,10]. Considering the values for the linearity and LOD values, the proposed method is more sensitive than published methods for assay of NTB alone or in combination with different drugs [4,8,12]. The developed method proved adequate for analysis of NTB in the biological fluids and in its pharmaceutical preparation.…”

“…Parise et al described an HPLC-mass spectrometry (MS) method for NTB in plasma and serum [7]. Furthermore, simultaneous assay of NTB with other antichronic myeloid leukemia drugs using solid phase extraction and ultra-performance liquid chromatographytandem mass (UPLC-MS/MS) [8], HPLC-UV [9][10], HPLC-MS/MS [11][12][13][14][15], and HPLC-MS [16,17] have been reported.…”

Review on Analytical Method Development and Validation by HPLC/Lc-MS of Selected Anti-Cancer Drugs

“…Andrea Davies, et al , [29] : Have been reported simultaneous estimation of several tyrosine Antonio D'Avolioa, et al, [30] :This reported method is simultaneous estimation of tyrosine kinase inhibitors like imatinib,dasatinib and nilotinib in human peripheral mononuclear cell by using HPLC-MS method.Chromtographic separation of drugs and internal standard Eva Kral et al, [31] :This reported method describes the simultaneous estimation of imatinib, nilotinib and dasatinib in dried blood spot by HPLC-MS.The chromatographic separaton of drugs achieved by C18 colmn and eluted with gradient mode through the mobile phase A was 0.1% formic acid in milliQ water and mobile phase B was 100% acetonitrile.The MS was operated in positive ESI mode and the drying temperature ,flow rate were 275 c and 5L/min.Mass spectrometer was operated in MRM mode.This method was validated in terms of linearity,selectivity, specificity,accuracy,precision and stability. …”

Recent developments in the chromatographic bioanalysis of approved kinase inhibitor drugs in oncology

“…During recent years dried blood spot (DBS) methods gain interest [31][32][33][34][35][36]. DBS, especially in combination with LC-MS/MS has many diverse applications, including screens for inherited metabolic disorders in newborn, workplace drug testing, therapeutic drug monitoring, toxicokinetics, and PKs in drug development [32].…”

“…With DBS, the type of material could introduce major variation in extraction efficiency. Not all reports about TKI bioanalysis mentioned the investigation of multiple paper-substrates [31,33], except for Rahavendran et al, reporting there was no effect between two different substrates [32], and Nijenhuis et al reported ionization enhancement for FTA DMPK-A, but not 903 protein saver cards. The FTA DMPK-A cards consist of cellulose, SDS and trometamol (TRIS).…”