Simultaneous measurement of total hemoglobin and its derivatives in blood using CO-oximeters: Analytical principles; Their application in selecting analytical wavelengths and reference methods; A comparison of the results of the choices made

Brunelle, Jacques A.; Degtiarov, Arkady M.; Moran, Robert F.; Race, Leonard A.

doi:10.3109/00365519609088624

Cited by 29 publications

(14 citation statements)

References 19 publications

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“…The instrument was subsequently marketed as the IL-182 CO-Oximeter, which monitored absorption at 548, 568 and 578 nm. Brunelle et al 52 discussed the analytical principles behind CO-oximeter design at length. The main factors that governed accuracy of performance were the availability of reference materials of known purity, such that the extinction coe¤-cients for each haemoglobin derivative could be established with con¢dence, and the selection of appropriate wavelengths.…”

Section: Automated Differential Spectrophotometry: Co-oximetersmentioning

confidence: 99%

Analysis of carbon monoxide

Widdop

2002

Ann Clin Biochem

100

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The degree of exposure to carbon monoxide is most often assessed by measuring the blood carboxyhaemoglobin saturation. This measurement is relevant to investigations of acute accidental or deliberate poisoning and of chronic exposure in a domestic or work place environment. Simple spectrophotometric methods based on differential protein precipitation or dithionite reduction are prone to interference from other haemoglobin pigments and are imprecise for low-level estimations. Automated spectrophotometric devices (CO-oximeters) that estimate simultaneously total haemoglobin, percentage oxyhaemoglobin and percentage carboxyhaemoglobin have acceptable accuracy for carboxyhaemoglobin saturation levels of 45% and are recommended for most clinical purposes. For the investigation of low-level exposure and the detection of increased haemolysis in neonates, more sensitive methods involving the release of carbon monoxide and its measurement by gas chromatography are required. Gas chromatographic methods are also appropriate when examining post-mortem blood samples where putrefaction or heat stress has resulted in a signi cant change in haemoglobin composition.

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Section: Automated Differential Spectrophotometry: Co-oximetersmentioning

confidence: 99%

Analysis of carbon monoxide

Widdop

2002

Ann Clin Biochem

100

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“…CO-oximters are specialized instruments for determining COHb, O 2 Hb and MetHb simultaneously by measuring the absorption of the sample at several different wavelengths (such as at 535, 585, 594, 626 nm) and then conduct multi-variable analysis in order to obtain relative concentrations (Mahoney et al 1993;Vreman and Stevenson 1994;Brunelle et al 1996;Yukawa et al 1997). CO-oximters are specialized instruments for determining COHb, O 2 Hb and MetHb simultaneously by measuring the absorption of the sample at several different wavelengths (such as at 535, 585, 594, 626 nm) and then conduct multi-variable analysis in order to obtain relative concentrations (Mahoney et al 1993;Vreman and Stevenson 1994;Brunelle et al 1996;Yukawa et al 1997).…”

Section: Spectrophotometric Methods Based On Cohb Absorptionmentioning

confidence: 99%

Gasotransmitters: Physiology and Pathophysiology

Hermann¹,

Ситдикова²,

Weiger³

2012

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“…Methaemoglobin also absorbs considerable light in the wavelength range (Fig. 4), although the profile and therefore colour is different (Brunelle et al. 1996).…”

Section: Introductionmentioning

confidence: 99%

Retinal Oximetry

Hardarson

2013

Acta Ophthalmologica

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Thesis for a doctoral degree at the University of Iceland. All right reserved. No part of this publication may be reproduced in any formEinar has played a major role in conceptualising and planning all of the studies described in this thesis. He has supervised all data analysis and has taken part in revising all of the papers, as well as writing the first draft of paper VII (Retinal oximetry in central retinal artery occlusion).My sincere thanks to my fellow oximetrists, Sindri Traustason and Olof Birna Olafsdottir. Sindri analysed the data from the study on dorzolamide and wrote the first draft of the paper in collaboration with Thor Eysteinsson and myself. Sindri also participated in all subsequent revisions. Olof has collaborated on other oximetry projects (not in this thesis). Thanks to both of them for all the collaboration and discussions on oximetry through the years.My sincere thanks to Maria Soffia Gottfredsdottir for sharing her expertise in glaucoma. Maria was instrumental in recruiting participants for both studies on glaucoma and did most of the clinical examinations as well as al of the glaucoma surgery. She participated in writing both papers on glaucoma.My sincere thanks to Thora Eliasbet Jonsdottir and Samy Basit for collaboration on the light and dark paper. Thora collaborated in gathering the data for the second part of the study and did the first analysis of the data. Samy helped designing both parts of the study and participated in writing the paper. My sincere thanks to all the staff at the Ophthalmology department of Landspı´tali for all the help, support and companionship through the years.Special thanks to the engineers, who made the measurements possible. The retinal oximeter, used here, is based on the work of James Beach and collaborators. James has continued to work with the group through the years. The software used for all analysis of oximetry images for this thesis is the work of Gisli Hreinn Halldorsson and Robert Arnar Karlsson, who have been exceptional collaborators through the years. The technical side of the project was originally run within the Department of Engineering but later in the company Oxymap. My thanks to the staff at Oxymap for collaboration on oximetry in general through the years.My thanks also to those who have collaborated on short-term oximetry projects, which are not in this thesis:

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Simultaneous measurement of total hemoglobin and its derivatives in blood using CO-oximeters: Analytical principles; Their application in selecting analytical wavelengths and reference methods; A comparison of the results of the choices made

Cited by 29 publications

References 19 publications

Analysis of carbon monoxide

Analysis of carbon monoxide

Gasotransmitters: Physiology and Pathophysiology

Retinal Oximetry

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