Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Hozumi, Shizuko; Ikezawa, Kana; Shoji, Atushi; Hirano‐Iwata, Ayumi; Bliss, T. V. P.; Sugawara, Masao

doi:10.1016/j.bios.2010.11.052

Cited by 9 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tip of the capillary sensor can be positioned in the middle between stimulation and recording electrodes for fEPSP measurements. The example of simultaneous measurements at CA1 of a hippocampal slice demonstrates that although no significant changes in a glutamate current is detected by application of 0.052 Hz (test stimuli), a transient change in the current is observed by application of 2 Hz stimulation, indicating enhanced release of L-glutamate in CA1 region (Hozumi et al, 2011). The Lglutamate level in region CA1 at 2 Hz stimulation obtained by in situ calibration ranged from 0.8 to 2.2 M (1.4 M as an average) from 5 independent measurements.…”

Section: Electric Stimulationmentioning

confidence: 97%

“…In addition, such interference disappears in the presence of a small amount of L-glutamate . The response to glutamine is significantly modified by using recombinant GluOx (Hozumi et al, 2011). The recombinant GluOx-based sensor suffers interference from glutamine only at high concentration above 300 M, and the response to glutamine is very weak (1.83 pAM an average between 300 and 500 M) in comparison with the response to Lglutamate (472 pA/M).…”

Section: Selectivitymentioning

confidence: 99%

“…Masao Sugawara and Atushi Shoji (2011). A Glass Capillary-Based Microsensor for L-Glutamate in in Vitro Uses, Microsensors, Prof. Igor Minin (Ed.…”

Section: How To Referencementioning

confidence: 99%

See 2 more Smart Citations

A Glass Capillary-Based Microsensor for L-Glutamate in in Vitro Uses

Sugawara¹,

Shoji²

2011

Microsensors

Self Cite

View full text Add to dashboard Cite

Section: Electric Stimulationmentioning

confidence: 97%

Section: Selectivitymentioning

confidence: 99%

See 1 more Smart Citation

A Glass Capillary-Based Microsensor for L-Glutamate in in Vitro Uses

Sugawara¹,

Shoji²

2011

Microsensors

Self Cite

View full text Add to dashboard Cite

“…It is noted that the effect of endogenous L-glutamate on calibrating the implanted sensor is negligible because the basal concentration of endogenous L-glutamate is known to be nM level. 16,17 Thus, the calibration of a patch sensor in an ACSF is valid even for an implanted sensor. For both cases, the integrated currents vs. concentration plots were sigmoid up to 5.0 μM with a half-maximum response (EC50) of ~2.3 μM (regarding the response to 5 μM L-glutamate as the maximum).…”

Section: Calibration Of An Implanted Patch Sensormentioning

confidence: 99%

Implantable Patch Sensor for l-Glutamate in Hippocampal Slices

et al. 2013

Self Cite

View full text Add to dashboard Cite

We describe an implantable patch sensor for monitoring L-glutamate in hippocampal slices in submerged and interface preparations. This patch sensor is prepared by excising the cell membrane in the CA3 region of a hippocampal slice in a submerged preparation, and then implanted in the target neuronal region (CA1) of mouse hippocampal slices. The lifetime of the sensor was 3 -8 min for the interface slice and 40 -50 min for the submerged slice. The calibration of an implanted sensor can be achieved by adding an L-glutamate solution to a bath (ACSF) solution. The monitoring of L-glutamate release in the CA1 region of mouse hippocampal slices under chemical stimulation with γ-aminobutyric acid (GABA) and potassium chloride (KCl) was demonstrated.

show abstract

“…Enzyme-based sensors have also been extensively used for in vivo and in vitro monitoring of extracellular L-glutamate [7,26,35,34,39,38,32,17,33]. However, few studies have reported on the simultaneous recording of L-glutamate currents at an enzyme sensor and electrophysiological signals [13,18,16].…”

Section: Introductionmentioning

confidence: 98%

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Ikegami

Hozumi

Shoji

et al. 2014

Sensing and Bio-Sensing Research

Self Cite

View full text Add to dashboard Cite

Keywords:L-glutamate sensor High-frequency stimulation Field excitatory postsynaptic potential Mouse hippocampal slice Long-term potentiation a b s t r a c t Real-time monitoring of L-glutamate released by high-frequency stimulation in region CA1 of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting L-glutamate currents from the recorded ones was described and applied for determining the level of extracellular L-glutamate released by 100 Hz stimulation. Recording of an L-glutamate current with a current sampling interval of 1 Hz was found to be useful for acquiring a Faradaic current that reflects L-glutamate level released by the highfrequency stimulation of 7 trains, each 20 stimuli at 100 Hz and inter-train interval of 3 s. The L-glutamate level was obtained as 15 ± 6 lM (n = 8) for the persistent enhancement of fEPSPs, i.e., the induction of longterm potentiation (LTP), and 3 ± 1 lM (n = 5) for the case of no LTP induction. Based on these observations, the level of the extracellular L-glutamate was shown to play a crucial role in the induction of LTP.

show abstract

Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Cited by 9 publications

References 21 publications

A Glass Capillary-Based Microsensor for L-Glutamate in in Vitro Uses

A Glass Capillary-Based Microsensor for L-Glutamate in in Vitro Uses

Implantable Patch Sensor for l-Glutamate in Hippocampal Slices

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Contact Info

Product

Resources

About