Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes

Zhou, Qianyu; Yuan, Fang; Zhang, Xiaohui; Zhou, Ying‐Lin; Zhang, Xin‐Xiang

doi:10.1039/c8sc00307f

Cited by 28 publications

(9 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These approaches have also been combined with microfluidic devices [ 51 , 61 , 94 , 95 ] and complementary metal–oxide–semiconductor (CMOS) devices [ 96 , 97 ] for the continuous and real-time detection of biomolecules. Additionally, new insights into the design and screening of synthetic receptors have been obtained using powerful selection methods such as systematic evolution of ligands by exponential enrichment (SELEX) and its alternatives [ 98 , 99 , 100 ], in addition to click chemistry [ 101 , 102 ], all of which will likely result in more sensitive and specific sensing of diverse biomarkers. These breakthroughs and innovations will undoubtedly lead to the emergence and discovery of new technologies and materials for disease diagnosis and environmental monitoring in the near future.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors

Kim

Park

Jung

et al. 2021

Sensors

View full text Add to dashboard Cite

Localized surface plasmon resonance (LSPR)-based biosensors have recently garnered increasing attention due to their potential to allow label-free, portable, low-cost, and real-time monitoring of diverse analytes. Recent developments in this technology have focused on biochemical markers in clinical and environmental settings coupled with advances in nanostructure technology. Therefore, this review focuses on the recent advances in LSPR-based biosensor technology for the detection of diverse chemicals and biomolecules. Moreover, we also provide recent examples of sensing strategies based on diverse nanostructure platforms, in addition to their advantages and limitations. Finally, this review discusses potential strategies for the development of biosensors with enhanced sensing performance.

show abstract

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors

Kim

Park

Jung

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…When a detection process fails to offer a desirable property (e.g., high specificity), the coupling of two or more SNV-sensitive processes in the design of the assay may be the solution . Designing assays with principles similar to protein-based SNV discrimination assays but without the involvement of proteins should be another area of future research. − Such research efforts can lead researchers toward the development of advanced protein-free detection schemes of reduced analysis cost and increased compatibility to a wider range of conditions. − The employment of different nanomaterials in the sensing designs with a view to improving the detection performance, particularly the specificity, sensitivity, and analysis speed, is also an active area of biosensing research and worth further investigation in the field of targeted SNV detection. , …”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Targeted Detection of Single-Nucleotide Variations: Progress and Promise

Abi

Safavi

2019

ACS Sens.

View full text Add to dashboard Cite

Advances in nucleic acid sequencing and genotyping technologies have facilitated the discovery of an increasing number of single-nucleotide variations (SNVs) associated with disease onset, progression, and response to therapy. The reliable detection of such disease-specific SNVs can ensure timely and effective therapeutic action, enabling precision medicine. This has driven extensive efforts in recent years to develop novel methods for the fast and cost-effective analysis of targeted SNVs. In this Review, we highlight the most recent and significant advances made toward the development of such methodologies.

show abstract

“…Fluorescence-based techniques can also be used for multiplex detection due to the availability of different fluorophores, , but the spectral overlapping of different fluorescent groups and the limited number of fluorescent reporters make high-throughput detection difficult. Meanwhile, capillary electrophoresis (CE) has been widely used for the multiplex detection of nucleic acids. − The capability of CE for multiplex detection depends on its ability to separate products with different sizes. Multiplex ligation-dependent probe amplification (MLPA) is a reliable and efficient technique for the detection of multiple gene mutations. , Specific probes with unique lengths were designed for different targets so that the PCR products of the ligated probes could be separated by CE.…”

mentioning

confidence: 99%

Ultrasensitive Multiplex Detection of Single Nucleotide Polymorphisms Based on Short-Chain Hybridization Combined with Online Preconcentration of Capillary Electrophoresis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Reliable multiple single nucleotide polymorphisms (SNPs) detection at low abundance is of great significance for disease diagnosis and biomedical research. Herein, we have developed a novel and simple method for multiple SNPs detection combining solid-phase capture by specific hybridization with online preconcentration of capillary gel electrophoresis-laser-induced fluorescence (CGE-LIF). The method presents an excellent performance due to its favorable traits: the solid-phase short-chain hybridization ensures the high specificity of SNP detection; the effective separation ability of CGE can easily achieve multiplex detection; the simple online preconcentration significantly improves the detection sensitivity of fluorescent probe by nearly 100-fold. For a single SNP target, the assay achieves a limit of detection as low as 0.01–0.02% for three different NRAS mutations in the same codon. For multiple SNP targets, as low as 0.05% abundance can be easily realized. Our method is simple, efficient, ultrasensitive, and universal for multiple SNPs detection without complex enzymatic or chemical ligation reaction, which shows great potential in early clinical diagnosis.

show abstract

Simultaneous multiple single nucleotide polymorphism detection based on click chemistry combined with DNA-encoded probes

Abstract: A novel strategy utilizing a DNA template-directed CuAAC click reaction to mimic a ligation reaction based on DNA ligase was successfully established for multiple SNP detection with high sensitivity and specificity.

Cited by 28 publications

References 61 publications

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors

Targeted Detection of Single-Nucleotide Variations: Progress and Promise

Ultrasensitive Multiplex Detection of Single Nucleotide Polymorphisms Based on Short-Chain Hybridization Combined with Online Preconcentration of Capillary Electrophoresis

Contact Info

Product

Resources

About