Simultaneous online SPE–HPLC–MS/MS analysis of docetaxel, temsirolimus and sirolimus in whole blood and human plasma

Navarrete, Alicia; Martínez-Alcázar, M. P.; Durán, Ignacio; Calvo, Emiliano; Valenzuela, Belén; Barbas, Coral; Garcı́a, Antonia

doi:10.1016/j.jchromb.2013.01.017

Cited by 34 publications

(33 citation statements)

References 29 publications

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“…19 Currently, metabolic proling plays an important role in the evaluation of drug toxicity in the drug trial phase, such as the determination of optimal drug doses through drug-level measurements. 20 For example, as a potential anticancer agent, CAT was analyzed by endogenous metabolites for a better understanding of the efficacy and toxicity of potential anticancer agent. 21 This method showed its advantages in monitoring chemical changes of living cells, especially some low molecular weight metabolites.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells

et al. 2017

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A novel aminoquinazolin derivative (named 9d), synthesized in our lab shows potential antitumor activity against A549 lung cancer cell lines. However, previous studies on the pharmacological mechanism of 9d mostly focused on cell and gene levels; the metabolic mechanism remains unknown. In this paper, an ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) based metabolomics approach was established to study the probable antitumor mechanism. Q-TOF MS and MS/MS were used to identify significantly different metabolites. 22 significantly different metabolites were observed between the 9d-treated A549 lung cells group and control group. They were involved in glycerophospholipid metabolism, glutathione metabolism, phenylalanine metabolism, cysteine and methionine metabolism, and aminoacyl-tRNA biosynthesis. The results showed that glutathione level and cell-membrane components phosphatidylcholines were decreased in 9d-treated cells, while their oxidative products, oxidized glutathione and lysophosphatidylcholines, were significantly increased. Further biological investigation showed an apparent accumulation of reactive oxygen species and a decrease in mitochondrial membrane potential.The results indicated that the aminoquinazolin derivative induced oxidative stress-mediated apoptosis.Moreover, phenylalanine metabolism suggested the up-regulation of L-phenylalanine might act as an endogenous drug carrier of 9d to improve cellular antiproliferation ability. The inhibition of aminoacyltransfer RNA and flow cytometry results indicated that cell cycle progression was blocked in the G1 phase, which was in accordance with the results obtained from some marketed aminoquinazolin derivatives. The above results proposed that 9d could induce oxidative stress and cell cycle arrest, which finally led to cell apoptosis in A549 cells. The study supplies a rapidly and highly active strategy to investigate the antitumor mechanism of drugs, which benefits its further improvement and development.

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Section: Introductionmentioning

confidence: 99%

Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells

et al. 2017

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“…11 and Table 6, respectively. Most methods for taxanes analysis are based on RPLC coupled to MS 305,309,[426][427][428][429][430][431][432][433][434][435][436][437][438][439][440][441][442] and UV detection. 138,269,[443][444][445][446][447][448][449][450][451][452][453] LODs lower than 0.05 ng mL −1 were reached for the analysis of docetaxel in blood using a column-switching method involving a preconcentration step on a trapping column, prior to LC-MS analysis.…”

Section: Taxanesmentioning

confidence: 99%

“…138,269,[443][444][445][446][447][448][449][450][451][452][453] LODs lower than 0.05 ng mL −1 were reached for the analysis of docetaxel in blood using a column-switching method involving a preconcentration step on a trapping column, prior to LC-MS analysis. 435 Paclitaxel in plasma was quantified down to a concentration of 45 ng mL −1 using an LLE-LC-UV procedure.…”

Section: Taxanesmentioning

confidence: 99%

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Antineoplastic drugs and their analysis: a state of the art review

Guichard

Guillarme

Bonnabry

et al. 2017

Analyst

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The number of patients suffering from cancer is constantly increasing and, consequently, the number of different chemotherapy treatments administered is increasing. Given the high reactivity and toxicity of antineoplastic drugs, analytical methods are required in all pharmaceutical fields, from drug development to their elimination in wastewater; including formulation quality control, environment and human exposure and therapeutic drug monitoring. The aim of this paper is to provide an overview of the analytical methods available for the determination of antineoplastic drugs in different matrices such as pharmaceutical formulations, biological and environmental samples. The applicability and performance of the reported methods will be critically discussed, with focus on the most commonly used antineoplastic drugs. Only conventional compounds and small molecules for targeted therapy will be considered in the present review.

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“…Consequently, this technique could considerably enrich analyte and significantly improve the sensitivity using a limited volume of sample, which is important for the analysis of trace components in extremely valuable samples in remote areas, such as Antarctic and Arctic areas. Therefore, the on-line extraction technique has been rapidly developed and widely used in the analysis of a variety of contaminants in environmental and biological samples since it was invented [13][14][15]. However, to the best of our knowledge, the analysis of perfluoroalkyl substances using on-line solid-phase extraction technique has been rarely reported.…”

Section: Introductionmentioning

confidence: 99%

An on-line solid phase extraction–liquid chromatography tandem mass spectrometry method for the determination of perfluoroalkyl substances in the Antarctic ice core samples

Shi

Pan

Liang

et al. 2015

Chinese Chemical Letters

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Simultaneous online SPE–HPLC–MS/MS analysis of docetaxel, temsirolimus and sirolimus in whole blood and human plasma

Cited by 34 publications

References 29 publications

Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells

Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells

Antineoplastic drugs and their analysis: a state of the art review

An on-line solid phase extraction–liquid chromatography tandem mass spectrometry method for the determination of perfluoroalkyl substances in the Antarctic ice core samples

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