Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology

Abstract

The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.

Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

“…is result indicated that the developed RSM model is practicable and can be used to describe the relationship between extraction factors and TFC in the ultrasound-assisted extraction of flavonoids from C. hindsii leaves. In addition, the TFC obtained in this study was found to be comparable to that in tomato pulp (21.52 mg QE/g) [33] and much higher than that in Dysphania ambrosioides (0.06 mg QE/g) [34] and other medicinal plants [35,36]. e study suggests that C. hindsii leaves are a promising source of flavonoids and the ultrasound-assisted extraction is an efficient process for extracting flavonoids from C. hindsii leaves to maximize TFC in a short extraction time and low temperature.…”

“…Regardless of strong activity, synthetic antioxidants may cause harmful side effects and hence the exploration for natural antioxidants has been immensely reinforced in recent years. In the previous studies, plant flavonoids have been shown to own robust antioxidant effects [35,36]. Because oxidative stress is a noticeably complicated process, different assays were used in examining the antioxidant effect of C. hindsii leaf extract.…”

Efficient Extraction and Structural Characterization of Hemicellulose from Sugarcane Bagasse Pith

“…p-values and F-values are used to verify the significance between coefficients. Specifically, Prob > F values less than 0.01 or 0.05 indicate that coefficients of the model are significant, and the values more than 0.05 indicate that coefficients of the model are not significant [29,30]. Therefore, F Value of 63.77 and Prob > F of less than 0.01 show that the model is significant, and there is only a 0.01% chance of such a large "Model F-value" due to noise in the model.…”

Response Surface Optimization of an Extraction Method for the Simultaneous Detection of Sulfamethoxazole and 17β-Estradiol in Soil

“…However, when the ultrasonic time was long, the obtained crude extract could be adsorbed back to the soil with the influence of soil-water partition coefficients, resulting in decreased recoveries [43,44]. This declining trend may also be because the prolonged ultrasonic time can degrade the extraction targets or accompany the dissolution of other impurities [45]. The highest recoveries (76.50% and 83.68%) appeared at 30 min.…”

“…The volume of the extraction buffer (10,20,30,40, and 50 mL), the pH of the extraction solution (3, 4, 5, 6, and 7), the ultrasonic temperature (15,25,35,45, and 55 • C), ultrasonic time (10, 20, 30, 40, 50 min), and the frequency of extraction (1, 2, 3, 4 and 5) were explored in this part. The effect of the single factor was tested with all other factors kept constant, and the experimental results were repeated three times.…”